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Process parameter optimization of pulsed laser deposition of hydroxyapatite coated polymer implant for better surface roughness and cell viability

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Abstract

In this work, the roughness of the implant was predicted by statistical and experimental methods. A customised bone implant was fabricated using the additive manufacturing process. Hydroxyapatite was deposited on the implant using Pulsed Laser Deposition. Taguchi philosophy was used to determine the optimal deposition condition. The characterization result suggests that the particles were deposited in the form of droplets with various particle sizes. Based on an analytical study, it was found that the Oxygen Partial Pressure was the most influential parameter, accounting for about 47.64% of the contribution, followed by Laser Energy (26.76%), Substrate Temperature (18.35%) and Coating Duration (6.98%), which were the least influential parameters on the surface roughness produced by HA coating. A cell line study was carried out on different surface roughness values obtained, and based on cell growth, an optimal surface roughness was predicted. The favourable surface roughness predicted by the Taguchi method was around Ra = 403 nm, whereas based on cell line studies, it was predicted around Ra = 384 nm. From both the experimental and simulation results, it is suggested that the surface roughness range of 384 to 403 nm offers better cell viability.

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References

  1. Schwartz SR (2020) Short implants: an answer to a challenging dilemma? Dental Clinics 64(2):279–290

    Google Scholar 

  2. Ogle OE (2015) Implant surface material, design, and osseointegration. Dent Clin N Am 59:505–520

    Article  Google Scholar 

  3. Bauer TW, Zhang Y (2016) Implants and implant reaction. Diagn Histopathol 22:384–396

    Article  Google Scholar 

  4. Mohamed-Nur A, Tran SD et al (2017) Biomaterial surface proteomic signature determines interaction with epithelial cells. Acta Biomater 54:150–163

    Article  Google Scholar 

  5. Goodman SB, Yao Z, Keeney M, Yang F (2013) The future of biologic coatings for orthopaedic implants. Biomaterials 34:3174–3183

    Article  Google Scholar 

  6. Singh G, Sharma S et al (2022) Impact of post-heat-treatment on the surface-roughness, residual stresses, and micromorphology characteristics of plasma-sprayed pure hydroxyapatite and 7%-Aloxite reinforced hydroxyapatite coatings deposited on titanium alloy-based biomedical implants. J Market Res 18:1358–1380

    Google Scholar 

  7. Tan H (2016) In vivo surface roughness evolution of a stressed metallic implant. J Mech Phys Solids 95:430–440

    Article  MathSciNet  Google Scholar 

  8. Klaas de Groot (2018) Bioceramics calcium phosphate. CRC Press

    Book  Google Scholar 

  9. Dave TV, Gaur G, Chowdary N, Joshi D (2018) Customized 3D printing: a novel approach to migrated orbital implant. Saudi J Ophthalmol 32:330–333

    Article  Google Scholar 

  10. Montgomery DC (2017) Design and analysis of experiments. John wiley & sons

    Google Scholar 

  11. Ross PJ, Ross PJ (1988) Taguchi techniques for quality engineering: loss function, orthogonal experiments, parameter and tolerance design (No. TS156 R12). McGraw-Hill, New York

    Google Scholar 

  12. Al G, Özdemir U, Aksoy Ö (2013) Cytotoxic effects of reactive blue 33 on allium cepa determined using Taguchi’s L8 orthogonal array. Ecotoxicol Environ Saf 98:36–40

    Article  Google Scholar 

  13. Chua CK, Leong KF (2014) 3D Printing and additive manufacturing: Principles and applications (with companion media pack)-of rapid prototyping. World Scientific Publishing Company

    Book  Google Scholar 

  14. Hariharan K, Arumaikkannu G (2016) Structural, mechanical and in vitro studies on pulsed laser deposition of hydroxyapatite on additive manufactured polyamide substrate. International journal of Bioprinting 2(2):85–96

    Google Scholar 

  15. Awasthi S, Pandey SK, Arunan E, Srivastava C (2021) A review on hydroxyapatite coatings for the biomedical applications: experimental and theoretical perspectives. Journal of Materials Chemistry B 9(2):228–249

    Article  Google Scholar 

  16. Hulshof FF, Zhao et al (2017) NanoTopoChip: high-throughput nanotopographical cell instruction. Acta Biomater 62:188–198

    Article  Google Scholar 

  17. Boyan BD, Lotz EM, Schwartz Z (2017) Roughness and hydrophilicity as osteogenic biomimetic surface properties. Tissue Eng Part A 23(23–24):1479–1489

    Article  Google Scholar 

  18. Zareh B, Gorji AH, Bakhshi M, Nourouzi S (2013) Study on the effect of forming parameters in sheet hydrodynamic deep drawing using FEM-based Taguchi method. Int J Adv Des Manuf Technol 67:87–99

    Google Scholar 

  19. Ferrari M, Fornasiero MC, Isetta AM (1990) MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J Immunol Methods 131:165–172

    Article  Google Scholar 

  20. Deligianni DD, Katsala N, Ladas S, Sotiropoulou D, Amedee J, Missirlis YF (2001) Effect of surface roughness of the titanium alloy Ti–6Al–4V on human bone marrow cell response and on protein adsorption. Biomaterials 22:1241–1251

    Article  Google Scholar 

  21. Huang L, Zhu C, Muntele CI, Zhang T, Liaw PK, He W (2015) Surface engineering of a Zr-based bulk metallic glass with low energy Ar-or Ca-ion implantation. Mater Sci Eng C 47:248–255

    Article  Google Scholar 

  22. Okuji S, Sekiya M, Nakabayashi M, Endo H, Sakudo N, Nagai K (2000) Surface modification of polymeric substrates by plasma-based ion implantation. Nucl Instrum Methods Phys Res B 242:353–356

    Article  Google Scholar 

  23. Li L, Crosby K, Sawicki M, Shaw LL, Wang Y (2012) Effects of surface roughness of hydroxyapatite on cell attachment and proliferation. J Biotechnol Biomater 2:150

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Mechanical Engineering, Dr. Mahalingam College of Engineering and Technology, Pollachi, Tamil Nadu, 642 003, India

    K. Hariharan

  2. Department of Mechanical Engineering, St. Joseph’s College of Engineering, Old Mahabalipuram Road, Chennai, Tamil Nadu, 600 119, India

    N. Sathishkumar

  3. Department of Manufacturing Engineering, College of Engineering Guindy, Anna University, Chennai, Tamil Nadu, 600 025, India

    G. Arumaikkannu

Authors
  1. K. Hariharan
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  2. N. Sathishkumar
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  3. G. Arumaikkannu
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Contributions

K. Hariharan – Conceptualization, Methodology, Investigation, Writing—Original Draft. N.Sathishkumar – Visualization, Data curation, Validation. G.Arumaikkannu—Resources, Formal analysis, Supervision, Project administration.

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Correspondence to K. Hariharan.

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Hariharan, K., Sathishkumar, N. & Arumaikkannu, G. Process parameter optimization of pulsed laser deposition of hydroxyapatite coated polymer implant for better surface roughness and cell viability. Prog Addit Manuf (2024). https://doi.org/10.1007/s40964-024-00629-6

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  • DOI: https://doi.org/10.1007/s40964-024-00629-6

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