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Synergistic Development of Biochips and Cell Preservation Methodologies: a Tale of Converging Technologies

  • Artificial Tissues (A Atala and JG Hunsberger, Section Editors)
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Abstract

Purpose of the Review

Over the past several decades, cryopreservation has been widely used to preserve cells during long-term storage, but advances in stem cell therapies, regenerative medicine, and miniaturized cell-based diagnostics and sensors are providing new targets of opportunity for advancing preservation methodologies. The advent of microfluidic-based devices is an interesting case in which the technology has been used to improve preservation processing, but as the devices have evolved to also include cells, tissues, and simulated organs as part of the architecture, the biochip itself is a desirable target for preservation. In this review, we will focus on the synergistic co-development of preservation methods and biochip technologies while identifying where the challenges and opportunities lie in developing methods to place on-chip biologics on the shelf, ready for use.

Recent Findings

Emerging studies are demonstrating that the cost of some biochips have been reduced to the extent that they will have high utility in point-of-care settings, especially in low resource environments where diagnostic capabilities are limited. Ice-free low temperature vitrification and anhydrous vitrification technologies will likely emerge as the preferred strategy for long-term preservation of bio-chips.

Summary

The development of preservation methodologies for partially or fully assembled biochips would enable the widespread distribution of these technologies and enhance their application.

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Acknowledgments

This work was supported in part by grant no. 5RO1GM101796 from the National Institutes of Health (NIH) to GDE.

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  1. Department of Mechanical Engineering and Engineering Sciences|, University of North Carolina at Charlotte, 9201 University City Blvd., Charlotte, NC, 28223, USA

    Shangping Wang & Gloria D. Elliott

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Wang, S., Elliott, G.D. Synergistic Development of Biochips and Cell Preservation Methodologies: a Tale of Converging Technologies. Curr Stem Cell Rep 3, 45–53 (2017). https://doi.org/10.1007/s40778-017-0074-8

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