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Relationship between drought tolerance with activities of antioxidant enzymes in sugarcane

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Abstract

The objective of this study was to compare capacity of antioxidant enzymes between Khon Kaen 3 (KK3), a drought-tolerant, and Suphanburi 72 (SP72), a drought-sensitive sugarcane variety. Young sugarcane plants were grown under controlled conditions. Water stress was imposed at 4 leaf stage by withholding water supply for 4, 6 and 8 days as compared to control, receiving adequate water supply. First fully expanded leaves were harvested and proteins were extracted for assaying the activities of ascorbate peroxidase (APX), peroxidase (POX) and catalase (CAT). APX was a major antioxidant system in leaves of both the sugarcane varieties as it accounted for 65 % in KK3 and 69 % in SP72 in scavenging H2O2. POX accounted for 27 and 23 % in KK3 and SP72, respectively in the removal of H2O2. Negligible scavenging activities of CAT were observed in both sugarcane varieties. APX activities in KK3 leaves were induced and maintained during progressive water stress and were 15 % higher than that in SP72. POX activities in KK3 were 30 % greater than that in SP72. In conclusion, drought tolerance in KK3 was, at least partially, due to the greater activities of APX and POX under water deficit stress as compared to those of SP72.

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Acknowledgments

The authors are grateful for funding provided by Royal Golden Jubilee Ph.D. Program (RGJ), Thailand Research Fund (Grant No. PHD/0117/2551). N. Ngamhui was a RGJ scholarship recipient. We are thankful to Mrs. Taksina Sansayawichai, Ms. Amarawan Tippayarak (KhonKaen Field Crops Research Center) and Mr. Manit Suknimit (Suphan Buri Agricultural Research and Development Center, Office of Agriculture Research and Development Region 5) for their kindness providing all knowledge about sugarcane and sugarcane stalks that were used in this research.

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Authors and Affiliations

  1. Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand

    N. Ngamhui & C. Akkasaeng

  2. Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    N. Tantisuwichwong

  3. Genome Institute, National Center for Genetic Engineering and Biotechnology, Pathumthani, 12120, Thailand

    S. Roytrakul

  4. Hawaii Agriculture Research Center, 94-340 Kunia Road, Waipahu, HI, 96797, USA

    Y. J. Zhu

  5. Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East–West Road, Honolulu, HI, 96822, USA

    Q. X. Li

Authors
  1. N. Ngamhui
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  2. N. Tantisuwichwong
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  3. S. Roytrakul
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  4. Y. J. Zhu
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  5. Q. X. Li
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  6. C. Akkasaeng
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Correspondence to C. Akkasaeng.

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Ngamhui, N., Tantisuwichwong, N., Roytrakul, S. et al. Relationship between drought tolerance with activities of antioxidant enzymes in sugarcane. Ind J Plant Physiol. 20, 145–150 (2015). https://doi.org/10.1007/s40502-015-0155-6

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  • DOI: https://doi.org/10.1007/s40502-015-0155-6

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