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HPLC-UV characterization, anticholinesterase, and free radical-scavenging activities of Rosa moschata Herrm. leaves and fruits methanolic extracts

  • Biochemistry & Physiology - Original Article
  • Published:
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Abstract

In the current research, we have investigated the antioxidant and cholinesterase inhibitory potentials of Rosa moschata Herrm. leaves and fruits methanolic extract (RmL.Ext and RmF.Ext) for the first time. To determine the rough estimate of phytochemical composition of extracts, the total phenolic and flavonoid contents were determined using standard methods. Moreover, the phenolic compounds present in the extracts were identified by HPLC-UV analysis. The cholinesterase activities were determined following the Ellman’s assay. The antioxidant activities of extracts were investigated using DPPH and ABTS assays. Significant anticholinesterase potentials were exhibited by RmL.Ext with IC50 of 40 and 110 µg/ml against AChE and BChE respectively. In addition, RmL.Ext also scavenged the DPPH (IC50 = 360 µg/ml) and ABTS (IC50 = 105 µg/ml) free radicals. Comparatively, the leaves extract was more potent than fruits extract. Malic acid, chlorogenic acid, quercetin, rutin, pyrogallol, mandelic acid, hydroxybenzoic acid, and morin were identified as the possible potent antioxidants present in extract. Based on the current results of the study, it may be inferred that RmL.Ext and RmF.Ext of R. moschata are rich in bioactive compounds responsible for the free radicals scavenging and cholinesterases inhibition and this plant may be a valuable candidate for the treatment of neurodegenerative disorders, like Alzheimer’s disease. However, further investigation in terms of isolation of bioactive compounds and their screening of toxicological effects in animal models are needed to validate the observed results.

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Abbreviations

RmL.Ext:

Rosa moschata leaves extract

RmF.Ext:

Rosa moschata fruit extract

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

ABTS:

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

IC50 :

Half maximal inhibitory concentration

AChE:

Acetylcholinesterase enzyme

BChE:

Butyrylcholinesterase enzyme

BGH:

Botanical Garden Herbarium

DAD:

Diode array detector

HPLC:

High-performance liquid chromatography

TPC:

Total phenolic content

TFC:

Total flavonoid contents

DTNB:

5,5′-Dithiobis-(2-nitrobenzoic acid)

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Acknowledgements

The authors are grateful to the Department of Botany and Pharmacy, University of Malakand for providing lab facilities.

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Authors and Affiliations

  1. Department of Biochemistry, University of Malakand, Chakdara, Dir (Lower), 18800, Khyber Pakhtunkhwa, Pakistan

    Nausheen Nazir & Muhammad Zahoor

  2. Department of Biological Sciences, National University of Medical Sciences, Rawalpindi, 46000, Pakistan

    Atif Ali Khan Khalil

  3. Department of Botany, University of Malakand, Chakdara, Dir (Lower), 18800, Khyber Pakhtunkhwa, Pakistan

    Mohammad Nisar

  4. Department of Pharmacy, Sarhad University of Information Technology, Peshawar, Pakistan

    Sajjad Ahmad

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  1. Nausheen Nazir
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  2. Atif Ali Khan Khalil
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  3. Mohammad Nisar
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  4. Muhammad Zahoor
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  5. Sajjad Ahmad
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Contributions

N.N carried out experimental work and plants collection, N.N and A.A.K.K perform literature search and manuscript preparation. M.N, M.Z, N.N, A.A.K.K and S.A refined the manuscript for publication. All authors have read and approved the manuscript.

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Correspondence to Mohammad Nisar.

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Nazir, N., Khalil, A.A.K., Nisar, M. et al. HPLC-UV characterization, anticholinesterase, and free radical-scavenging activities of Rosa moschata Herrm. leaves and fruits methanolic extracts. Braz. J. Bot 43, 523–530 (2020). https://doi.org/10.1007/s40415-020-00635-2

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  • DOI: https://doi.org/10.1007/s40415-020-00635-2

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