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Polymorphisms of Beta2-Adrenergic Receptor Gene in Serbian Asthmatic Adults: Effects on Response to Beta-Agonists

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Abstract

Background and Objectives

Polymorphisms of beta2-adrenergic receptor gene (ADRB2) are clinically relevant for several reasons, including as a risk factor for asthma development/severity and predicting the effectiveness of treatment with beta2-agonists in reducing asthma symptoms. The aim of this study was to examine the association between ADRB2 gene polymorphisms and asthma in the Serbian population, and to evaluate the therapeutic response in relation to the ADRB2 genotype.

Methods

The study included 171 patients with asthma and 101 healthy subjects as the control group. Genotyping of Arg16Gly and Gln27Glu polymorphisms was performed by direct sequencing of polymerase chain reaction (PCR) products.

Results

In Serbian adults, carriers of the 27Gln allele and 27Gln/Gln genotype were at higher risk of asthma [odds ratio (OR) 2.5, 95 % confidence interval (CI) 1.6–3.8, and OR 3.00, 95 % CI 1.7–5.3, respectively], while the presence of the 27Glu allele and 27Gln/Glu genotype were found to be protective of asthma (OR 0.4, 95 % CI 0.3–0.6, and OR 0.3, 95 % CI 0.1–0.7, respectively). Furthermore, we found that the presence of the 27Gln allele in asthmatics younger than 50 years leads to a better response to therapy with long-acting beta2-agonists (LABA) in combination with prevailing low and moderate doses of inhaled corticosteroids (ICS), while carriers of the 27Glu allele over 50 years old are more likely to respond to LABA + ICS therapy.

Conclusion

We identified that in Serbian adults the 27Gln allele and 27Gln homozygosity are risk factors for asthma, which may be of clinical interest in disease prevention. The finding that younger carriers of the 27Gln allele respond better to LABA + ICS therapy may be utilized in personalized asthma treatment.

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Acknowledgments

This work was supported by Grant 173008 of the Ministry of Education, Science and Technological Development, Republic of Serbia.

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Department of Pulmonology, Zvezdara University Medical Centre, Belgrade, Serbia

    Natasa Petrovic-Stanojevic & Vesna Dopudja-Pantic

  2. Department of Internal Medicine, School of Dental Medicine, University of Belgrade, Belgrade, Serbia

    Natasa Petrovic-Stanojevic

  3. Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe, 450, 11221, Belgrade, Serbia

    Aleksandra Topic

  4. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia

    Aleksandra Nikolic, Marija Stankovic & Dragica Radojkovic

  5. Clinic for Pulmonary Diseases, Clinical Centre of Serbia, School of Medicine, University of Belgrade, Belgrade, Serbia

    Branislava Milenkovic

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  1. Natasa Petrovic-Stanojevic
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  2. Aleksandra Topic
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  3. Aleksandra Nikolic
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Correspondence to Aleksandra Topic.

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Petrovic-Stanojevic, N., Topic, A., Nikolic, A. et al. Polymorphisms of Beta2-Adrenergic Receptor Gene in Serbian Asthmatic Adults: Effects on Response to Beta-Agonists. Mol Diagn Ther 18, 639–646 (2014). https://doi.org/10.1007/s40291-014-0116-1

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  • DOI: https://doi.org/10.1007/s40291-014-0116-1

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