Skip to main content
SpringerLink
Log in

Dark-vacuole Bodies Studying by High-resolution Label-free Microscopy Assisted with Fluorescence Technology

  • Article
  • Published:
Chemical Research in Chinese Universities Aims and scope

Abstract

The intracellular logistics system, consisting of vesicles, plays a crucial role in cellular transport. However, there is a lack of research on the types and functions of intracellular vesicles, and new technologies are needed for further investigation. Recently, researchers have discovered a new subcellular structure known as Dark-vacuole bodies. The composition, function, and potential synergy with other organelles of these Dark-vacuole bodies remain unclear. In this study, we utilized the high-resolution label-free Fourier ptychographic microscopy, developed by our research group, along with fluorescence confocal technology, to study and analyze Dark-vacuole bodies. Our findings provide evidence of the influence of Dark-vacuole bodies on the morphology, distribution, movement, and cell cycle of living cells. Specifically, we analyzed the effects of drug induced stimulation of lipid drops and endosomes, promotion of cell endocytosis, and induction of cellular senescence on Dark-vacuole bodies. Our results indicate that Dark-vacuole bodies show little correlation with lipid drops and endocytosis vesicles, but are significantly associated with late endosomes. Furthermore, cellular senescence leads to a significant increase in the number and size of Dark-vacuole bodies. This study serves as a foundation for further confirming the nature of Dark-vacuole bodies as new organelles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Das Gupta A., Krawczynska N., Nelson E. R., Endocrinology, 2021, 162, doi: https://doi.org/10.1210/endocr/bqab133

  2. Cui L., Li H., Xi Y., Hu Q., Liu H., Fan J., Xiang Y., Zhang X., Shui W., Lai Y., Mol. Biomed., 2022, 3, 29

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Palade G., Science, 1975, 189, 867

    Article  CAS  PubMed  Google Scholar 

  4. Winters C. M., Hong-Brown L. Q., Chiang H. L., J. Biol. Chem., 2020, 295, 2650

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Ginini L., Billan S., Fridman E., Gil Z., Cells, 2022, 11, doi: https://doi.org/10.3390/cells11091375

  6. Xu H., Wang H., Chem. Res. Chinese Universities, 2021, 37(4), 829

    Article  CAS  Google Scholar 

  7. Verweij F. J., Balaj L., Boulanger C. M., Carter D. R. F., Compeer E. B., D’Angelo G., El Andaloussi S., Goetz J. G., Gross J. C., Hyenne V., Krämer-Albers E. M., Lai C. P., Loyer X., Marki A., Momma S., Nolte-’t Hoen E. N. M., Pegtel D. M., Peinado H., Raposo G., Rilla K., Tahara H., Théry C., van Royen M. E., Vandenbroucke R. E., Wehman A. M., Witwer K., Wu Z., Wubbolts R., van Niel G., Nat. Methods, 2021, 18, 1013

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Dong D., Huang X., Li L., Mao H., Mo Y., Zhang G., Zhang Z., Shen J., Liu W., Wu Z., Liu G., Liu Y., Yang H., Gong Q., Shi K., Chen L., Light Sci. Appl., 2020, 9, 11

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Zheng G., Horstmeyer R., Yang C., Nat Photonics, 2013, 7, 739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Zheng G., Ou X., Horstmeyer R., Chung J., Yang C., Optics and Photonics News, 2014, 25, 26

    Article  Google Scholar 

  11. Jensen J. A., Tomov B. G., Haslund L. E., Panduro N. S., Sorensen C. M., IEEE Trans Ultrason Ferroelectr Freq Control, 2023, 70, 708

    Article  PubMed  Google Scholar 

  12. Gao P., Pedrini G., Osten W., Opt. Lett., 2013, 38, 1328

    Article  PubMed  Google Scholar 

  13. Maire G., Giovannini H., Talneau A., Chaumet P. C., Belkebir K., Sentenac A., Opt. Lett., 2018, 43, 2173

    Article  PubMed  Google Scholar 

  14. Ancora D., Di Battista D., Giasafaki G., Psycharakis S. E., Liapis E., Ripoll J., Zacharakis G., Methods, 2018, 136, 81

    Article  CAS  PubMed  Google Scholar 

  15. Sun M., Chen X., Zhu Y., Li D., Mu Q., Xuan L., Opt. Express, 2019, 27, 24161

    Article  PubMed  Google Scholar 

  16. Pan A., Zhang Y., Wen K., Zhou M., Min J., Lei M., Yao B., Opt. Express, 2018, 26, 23119

    Article  CAS  PubMed  Google Scholar 

  17. Shen Q., Xu R., Wang Z., Zhao T., Zhou Y., Xu Y., Yang Z., Lei M., Meng L., Dang D., Chem. Res. Chinese Universities, 2021, 37(1), 143

    Article  CAS  Google Scholar 

  18. Sun L., Wang Y., Zhang H., Min C., Zhang Y., Zhang C., Xin Z., Zhu S., Yang Y., Burge R. E., Yuan X., ACS Sens., 2020, 5, 510

    Article  CAS  PubMed  Google Scholar 

  19. Xing F., Meng G. X., Zhang Q., Pan L. T., Wang P., Liu Z. B., Jiang W. S., Chen Y., Tian J. G., Nano Lett., 2014, 14, 3563

    Article  CAS  PubMed  Google Scholar 

  20. Marquet P., Rappaz B., Magistretti P. J., Cuche E., Emery Y., Colomb T., Depeursinge C., Opt. Lett., 2005, 30, 468

    Article  PubMed  Google Scholar 

  21. Song N., Xiao P., Ma K., Kang M., Zhu W., Huang J., Wang D., Tang B. Z., Chem. Res. Chinese Universities, 2021, 37(1), 52

    Article  CAS  Google Scholar 

  22. Krahmer N., Guo Y., Wilfling F., Hilger M., Lingrell S., Heger K., Newman H. W., Schmidt-Supprian M., Vance D. E., Mann M., Farese R. V., Jr., Walther T. C., Cell Metab, 2011, 14, 504

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Li S., Pan Y., Teng H., Shan Y., Yang G., Wang H., Chem. Res. Chinese Universities, 2022, 38(3), 838

    Article  CAS  Google Scholar 

  24. Dufès C., Al Robaian M., Somani S., Ther. Deliv., 2013, 4, 629

    Article  PubMed  Google Scholar 

  25. Daniels T. R., Delgado T., Rodriguez J. A., Helguera G., Penichet M. L., Clin. Immunol., 2006, 121, 144

    Article  CAS  PubMed  Google Scholar 

  26. Xu H., Zhang J., Zhou Y., Zhao G., Cai M., Gao J., Shao L., Shi Y., Li H., Ji H., Zhao Y., Wang H., Research, 2022, 2022, 9835035

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Zhang W., Li J., Suzuki K., Qu J., Wang P., Zhou J., Liu X., Ren R., Xu X., Ocampo A., Yuan T., Yang J., Li Y., Shi L., Guan D., Pan H., Duan S., Ding Z., Li M., Yi F., Bai R., Wang Y., Chen C., Yang F., Li X., Wang Z., Aizawa E., Goebl A., Soligalla R. D., Reddy P., Esteban C. R., Tang F., Liu G. H., Belmonte J. C., Science, 2015, 348, 1160

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Wu Z., Zhang W., Song M., Wang W., Wei G., Li W., Lei J., Huang Y., Sang Y., Chan P., Chen C., Qu J., Suzuki K., Belmonte J. C. I., Liu G. H., Protein Cell, 2018, 9, 333

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Chang B. D., Broude E. V., Dokmanovic M., Zhu H., Ruth A., Xuan Y., Kandel E. S., Lausch E., Christov K., Roninson I. B., Cancer Res., 1999, 59, 3761

    CAS  PubMed  Google Scholar 

  30. Hayakawa A., Leonard D., Murphy S., Hayes S., Soto M., Fogarty K., Standley C., Bellve K., Lambright D., Mello C., Corvera S., Proceedings of the National Academy of Sciences, 2006, 103, 11928

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the Scientific Instrument Developing Project of the Chinese Academy of Sciences (No. ZDKYYQ20220005) and the National Natural Science Foundation of China (Nos. 22150003, 21727816, and 21721003).

Author information

Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China

    Xiangyu Liu, Jinrui Zhang, Haijiao Xu, Lina Shao & Hongda Wang

  2. School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, P. R. China

    Xiangyu Liu & Hongda Wang

  3. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, P. R. China

    Hongda Wang

Authors
  1. Xiangyu Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jinrui Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Haijiao Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lina Shao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hongda Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Lina Shao or Hongda Wang.

Ethics declarations

WANG Hongda is a youth executive editorial board member for Chemical Research in Chinese Universities and was not involved in the editorial review or the decision to publish this article. The authors declare no conflicts of interest.

Electronic Supplementary Information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, X., Zhang, J., Xu, H. et al. Dark-vacuole Bodies Studying by High-resolution Label-free Microscopy Assisted with Fluorescence Technology. Chem. Res. Chin. Univ. (2024). https://doi.org/10.1007/s40242-024-3271-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s40242-024-3271-x

Keywords

Search

Navigation