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Morphological Changes and the Expression of Oxidoreductase Genes of Brassica napus in Deep Culture Media Containing PEG

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The aim of this research was to investigate the expression of important oxidoreductase genes involved in drought stress tolerance in two Brassica napus cultivars. For this purpose, morphological traits and the expression of four oxidoreductase genes within 0, 3, 6, 12, 24, 48 and 72 h after culture in deep culture medium containing 0, 3, 6, 12 and 15% polyethylene glycol 6000 (PEG 6000) were investigated. Results revealed that in both sensitive and tolerant cultivars, there was a declining trend in not only the leaf weight and area but also the stem weight and length with increasing the PEG level. In both cultivars, changes in the expression of NDH were approximately the same at different PEG levels. The tolerant cultivar showed higher expression of FNR than the sensitive cultivar. The expression of GR1 and GPX1 genes was higher in Hyola 308 than SLM 046.

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Acknowledgements

The authors are grateful to The Guilan University, Biotechnology Laboratory collaborators for the constant support throughout this study.

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Authors and Affiliations

  1. Guilan University, Rasht, Iran

    F. S. Seyed Ebrahimi, S. H. Hassani Kumleh & M. H. Rezadoost

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  1. F. S. Seyed Ebrahimi
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  2. S. H. Hassani Kumleh
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  3. M. H. Rezadoost
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Correspondence to S. H. Hassani Kumleh.

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Significance statement

The authors tried to simulate bioreactor conditions by using suspension deep culture. For understanding exact gene expression and phenotypic changes, drought levels (0, 3, 6, 12 and 15% w/v) and harvesting times (0, 3, 6, 12, 24, 48 and 72 h) were exclusively extended.

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Ebrahimi, F.S.S., Kumleh, S.H.H. & Rezadoost, M.H. Morphological Changes and the Expression of Oxidoreductase Genes of Brassica napus in Deep Culture Media Containing PEG. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 1311–1318 (2019). https://doi.org/10.1007/s40011-018-1050-5

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  • DOI: https://doi.org/10.1007/s40011-018-1050-5

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