Abstract
The present study reports on the microbial dynamics and the accompanied nutritional changes during the fermentation process of Namsing. Fermentation of fish during Namsing preparation revealed a significant increase in mineral content with the passage of fermentation time. Changes in microbial dynamics such as mesophilic bacteria, lactic acid bacteria (LAB) and Enterobacteriaceae were monitored during the indigenous fermentation process. The mesophilic bacterial count of Namsing was 3.44 log CFU/g on the first day which increased to 8.2 log CFU/g on the 7th day and decreased gradually to 3.36 log CFU/g on the 28th day of fermentation. Yeasts and moulds were not detected during the fermentation period. Lactic acid bacteria were not detected until the 21st day. However on the 28th day, the lactic acid bacteria were recorded in the range of 1–2 log CFU/g. Coliform or other gram-negative bacteria were detected in the range of 2–2.8 log CFU/g till the end of fermentation. A total of 27 isolates were selected for further study based on their colony morphology and identified through biochemical and molecular analysis. The isolates belonged to the genus Bacillus (40.7%), Lactobacillus (7.4%), Kurthia (14.8%), Providencia (11%), Wohlfahrtiimonas (7.4%), Paenibacillus (3%), Staphylococcus (3%) Klebsiella (7.4%) and Vagococcus (3%). Among the 27 isolates, 11 isolates were further screened quantitatively for protease activity and using casein as substrate. The highest protease activity (15.60 ± 0.31 units/mL) was shown by the isolate Kurthia gibsonii (N11).
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References
Sathe GB, Mandal S (2016) Fermented products of India and its implication: a review. Asian J Dairy Food Res 35:1–9. https://doi.org/10.18805/ajdfr.v35i1.9244
Tamang JP, Tamang N, Thapa S et al (2012) Microorganisms and Nutritional value of Ethnic fermented foods and alcoholic beverages of North East India. Indian J Tradit Knowl 11:7–25
Goswami G, Deka P, Das P et al (2017) Diversity and functional properties of acid-tolerant bacteria isolated from tea plantation soil of Assam. 3 Biotech. https://doi.org/10.1007/s13205-017-0864-9
Thapa N (2016) Ethnic fermented and preserved fish products of India and Nepal. J Ethn Foods 3:69–77. https://doi.org/10.1016/j.jef.2016.02.003
Tamang JP (2015) Naturally fermented ethnic soybean foods of India. J Ethn Foods 2:8–17. https://doi.org/10.1016/j.jef.2015.02.003
Majumdar RK, Roy D, Bejjanki S, Bhaskar N (2016) An overview of some ethnic fermented fish products of the Eastern Himalayan region of India. J Ethn Foods 3:276–283. https://doi.org/10.1016/j.jef.2016.12.003
AOAC (1999) Official methods of analysis, 16th edn. The Association of Official Analytical Chemists, Gaithersburg
AOAC (2000) Official methods of analysis, 17th edn. The Association of Official Analytical Chemists, Maryland, USA
AOAC (1984) Official methods of analysis, 14th edn. The Association of Official Analytical Chemists, Washington DC
Tamura K, Stecher G, Peterson D et al (2013) MEGA6: molecular Evolutionary Genetics Analysis Version 6.0. Mol Biol Evol 30:2725–2729. https://doi.org/10.1093/molbev/mst197
Cupp-Enyard C (2008) Sigma’s non-specific protease activity assay—casein as a substrate. J Vis Exp. https://doi.org/10.3791/899
Beaumont M (2002) Flavouring composition prepared by fermentation with Bacillus spp. Int J Food Microbiol 75:189–196
Richard MJ, Roussel AM (1999) Micronutrients and ageing: intakes and requirements. Proc Nutr Soc 58:573–578
Yirankinyuki FF, Lamayi DW, Sadiq BA, Yakubu MU (2013) Proximate and some minerals analysis of Colocasia Esculenta (TARO) leaves. J Med Biol Sci 3:8–14
Goswami G, Baruah H, Boro RC, Barooah M (2016) Fermentation reduces anti-nutritional content and increases mineral availability in Poita bhat. Asian J Chem 28:1929–1932. https://doi.org/10.14233/ajchem.2016.19820
Mohite BV, Chaudhari GA, Ingale HS, Mahajan VN (2013) Effect of fermentation and processing on in vitro mineral estimation of selected fermented foods. Int Food Res J 20:1373–1377
Nazmanara Khanum M, Takamura H, Matoba T (1999) Nutritional composition of a semi-fermented fish product (Chapa Shutki) in Bangladesh. J Home Econ Jpn 50:703–712
Osman OA, Sulieman AME, Elkhalifa EA, Mustafa WA (2012) Chemical and microbiological characteristics of fermented fish product, Fassiekh. Food Pub Heal 2:213–218. https://doi.org/10.5923/j.fph.20120206.05
Thapa N, Pal J, Tamang JP (2006) Phenotypic identification and technological properties of lactic acid bacteria isolated from traditionally processed fish products of the Eastern Himalayas. Int J Food Microbiol 107:33–38. https://doi.org/10.1016/j.ijfoodmicro.2005.08.009
Whitman WB, Parte AC (2009) The firmicutes, 2nd edn. Springer, New York
Garrity GM (2005) Bergey’s manual of systematic bacteriology. The proteobacteria. Springer, Berlin
Borsodi AK, Kiss RI, Cech G et al (2010) Diversity and activity of cultivable aerobic planktonic bacteria of a saline Lake located in Sovata, Romania. Folia Microbiol (Praha) 55:461–466. https://doi.org/10.1007/s12223-010-0077-7
Garrity GM, Holt JG (2001) The road map to the manual. Bergey’s manual® systematic bacteriology. Springer, New York, pp 119–166
Ahmed S, Dora KC, Sarkar S et al (2013) Quality analysis of shidal—A traditional fermented fish product of Assam, North-East India. Indian J Fish 60:117–123
Stackebrandt E, Frederiksen W, Garrity GM et al (2002) Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047. https://doi.org/10.1099/ijs.0.02360-0.02360
Mi-na U, Lee C-H (1996) Isolation and identification of Staphylococcuss sp. from Korean fermented fish products. Microbiol Biotechnol 6:340–346
Schmidtke LM, Carson J (1994) Chararacteristics of Vagococcus salmoninarum isolated from diseased salmonid fish. J Appl Bacteriol 77:229–236. https://doi.org/10.1111/j.1365-2672.1994.tb03068.x
Shewmaker PL, Steigerwalt AG, Morey RE et al (2004) Vagococcus carniphilus sp. nov., isolated from ground beef. Int J Syst Evol Microbiol 54:1505–1510. https://doi.org/10.1099/ijs.0.02908-0
Pereira Rodarte M, Dias, Vilela DM, Schwan RF (2011) Proteolytic activities of bacteria, yeasts and filamentous fungi isolated from coffee fruit (Coffea arabica L.). Acta Sci Agron Mar 33:457–464. https://doi.org/10.4025/actasciagron.v33i3.6734
Chantawannakul P, Oncharoen A, Klanbut K et al (2002) Characterization of proteases of Bacillus subtilis strain 38 isolated from traditionally fermented soybean in Northern Thailand. Sci Asia 28:241–245
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The authors are grateful to the Department of Agricultural Biotechnology and DBT-AAU center, Assam Agricultural University (AAU) for providing the necessary facilities to carry out the present work.
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Chowdhury, N., Goswami, G., Hazarika, S. et al. Microbial Dynamics and Nutritional Status of Namsing: A Traditional Fermented Fish Product of Mishing Community of Assam. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 1027–1038 (2019). https://doi.org/10.1007/s40011-018-1022-9
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DOI: https://doi.org/10.1007/s40011-018-1022-9