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AuNP-Loaded Electrospinning Membrane Cooperated with CDs for Periodontal Tissue Engineering

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Guided bone regeneration (GBR) is commonly used to regenerate periodontal tissue. However, the bone inductivity and antibacterial properties of the GBR membranes currently in use are severely limited. This issue can be resolved by loading growth factors and antibiotics. Bioactive substitutes, such as Au nanoparticles (AuNPs) and carbon quantum dots (CDs), were proposed to prevent the denaturation of osteogenic growth factors and the induction of antibacterial drug resistance.

Methods:

Ornidazole was initially used as the raw material to prepare the CDs, followed by the incorporation of an optimal ratio of nanoparticles to produce the electrospun membrane doped with AuNPs and novel traceable antibacterial CDs. The morphology of the membrane was characterized. The adhesion, proliferation, and osteogenic differentiation of cells on the membrane were evaluated in vitro. The antimicrobial characteristics of the membrane were also investigated. The electrospun membrane was implanted into a rat skull defect model in vivo to investigate its osteogenic potential.

Results:

The blending of nanomaterials did not affect the micro morphology of the fiber, resulting in enhanced mechanical properties. Membranes doped with AuNPs and CDs exhibited excellent biocompatibility, increased ALP activity, improved calcified nodules, and increased expression of osteogenic-associated proteins, in addition to pronounced antibacterial effects. The membrane also demonstrated excellent osteogenic characteristics in rat models.

Conclusion:

The synergistic effect of loaded AuNPs electrospun fiber membrane with CDs can promote periodontal bone regeneration and exert antibacterial activity.

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Data availability statement

The data presented in this study are available on request from all the authors.

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Acknowledgements

This work was supported by a grant from the National Natural Science Foundation of China (NSFC, no. 81972536), the Key Project of Health Commission of Jiangsu Province (no. K2019013), the Natural Science Foundation of Jiangsu Higher Education Institutions of China (no. 20KJA320003), A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD, 2018-87), and Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX20_1409).

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Author notes

  1. Jie Wang and Hang Zhang have contributed equally to this work.

Authors and Affiliations

  1. Jiangsu Key Laboratory of Oral Diseases, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing Medical University, No. 140, Hanzhong Road, Nanjing, 210029, China

    Jie Wang, Hang Zhang, Yan Wang, Xiang Liu, Weiwen Zhu, Fei Jiang, Size Li & Laikui Liu

  2. Department of General of Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, 210029, China

    Jie Wang & Fei Jiang

  3. Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, 210029, China

    Laikui Liu

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  1. Jie Wang
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Correspondence to Laikui Liu.

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Ethical statement

The study was approved by the Ethics Committee of Nanjing Medical University (PJ2021-145–001). The animal experiment was approved by the Institutional Animal Care and Use Committee of Nanjing Medical University (IACUC approval No. 2012010).

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Wang, J., Zhang, H., Wang, Y. et al. AuNP-Loaded Electrospinning Membrane Cooperated with CDs for Periodontal Tissue Engineering. Tissue Eng Regen Med 20, 1091–1108 (2023). https://doi.org/10.1007/s13770-023-00583-4

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  • DOI: https://doi.org/10.1007/s13770-023-00583-4

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