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Bladder Augmentation Using Lyoplant®: First Experimental Results in Rats

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Congenital defects of the urinary bladder (micro- or contracted bladder, bladder exstrophy) remain a challenging problem for pediatric surgeons. Even when conservative treatment options are fully exhausted, irreversible renal dysfunction can be observed in a large number of cases that can even lead to chronic renal failure and the need for kidney transplantation. To protect kidney function bladder augmentation using intestinal tissue is commonly applied as the standard treatment method. However due to the unphysiological nature of intestinal tissue a number of problems and complications such as urinary tract infections or bladder stone formation limit the clinical success of this approach. Moreover a number of substitutes for the implementation of a bladder augmentation have been tested without success to date. Here we used an experimental model to test wether the biocompatible collagen mesh Lyoplant may be a suitable candidate for bladder augmentation.

Methods:

We implanted a biocompatible collagen mesh (Lyoplant®) in a bladder defect rat model for bladder augmentation (Lyoplant®-group: n = 12; sham group n = 4). After 6 weeks the abdomen was reopened and the initial implant as well as the bladder were resected for histological and immunohistochemical examination.

Results:

All but one rat exhibited physiological growth and behaviour after the operation without differences between the Lyoplant®-group (n = 12) and the sham group (n = 3). One rat from the sham group had to be excluded because of a suture leakage. No wound healing complications, wound infections and no herniation were observed. After 5 weeks the implants showed an adequate incorporation in all cases. This was confirmed by immunohistological analyses where a significant cell infiltration and neovascularization was observed.

Conclusion:

In summary, Lyoplant® appears to be a promising tool in experimental bladder augmentation/regeneration in rats.

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Acknowledgement

The author’s thanks Mrs. Chodnesvska for the technical support.

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Authors and Affiliations

  1. Pediatric Surgery-, Pediatric Trauma - and Pediatric Urology Unit, Department of General-, Visceral-, Transplant-, Vascular- and Pediatric Surgery, University Hospital Wuerzburg, Würzburg, Germany

    F. Winde, K. Backhaus & Th. Meyer

  2. Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK

    J. A. Zeitler

  3. Experimental Surgery Unit, Department of General-, Visceral-, Transplant-, Vascular- and Pediatric Surgery, University of Wuerzburg, Würzburg, Germany

    N. Schlegel

Authors
  1. F. Winde
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  2. K. Backhaus
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  3. J. A. Zeitler
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  4. N. Schlegel
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  5. Th. Meyer
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Correspondence to Th. Meyer.

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The authors declare that they have no conflict of interest.

Ethical statement

The study was performed according to a protocol approved by the local committee for animal use and care (Regierung von Unterfranken: 55.2-2531.01-01/12).

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Winde, F., Backhaus, K., Zeitler, J.A. et al. Bladder Augmentation Using Lyoplant®: First Experimental Results in Rats. Tissue Eng Regen Med 16, 645–652 (2019). https://doi.org/10.1007/s13770-019-00209-8

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  • DOI: https://doi.org/10.1007/s13770-019-00209-8

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