Abstract
To develop decellularized heart valve scaffold from porcine for heart valve regeneration. Porcine heart valves were decellularized with unique optimized approach by using 1% sodium dodecyl sulfate solution and 5% dimethyl sulfoxide for the first time. Effect of decellularization process on scaffold were characterized by hematoxylin–eosin, 4′,6-diamidino-2-phenylindole, Masson’s trichrome, alcian blue staining and scanning electron microscopy for extracellular matrix (ECM) analysis in scaffold. The results showed that developed protocol for decellularization of heart valve scaffold shown complete removal of all cellular components, without changing the properties of ECM. The developed protocol was successfully used for heart valve ECM scaffolds development from porcine. The developed protocol seems to be promising solution for the heart valve tissue engineering application.
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Acknowledgements
The authors thank D. Y. Patil University for financial support. Authors are also thankful for department of physics Shivaji University Kolhapur for extending SEM facility. Dr. Meghnad G Joshi acknowledge Department of Science and Technology (DST), Govt. of India (SB/SO/HS/0198/2013).
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All authors declare that they have no ethical issues for human and animal right in the ethical statement section. The present study we do not have any ethical issues for human and animal right. The present study does not involve use of human and animal subjects. The heart valves were collected from slaughter house. The study protocol was approved by the Institutional Research committee. The approval number is DMCK 93/2017.
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Tardalkar, K., Desai, S., Adnaik, A. et al. Novel Approach Toward the Generation of Tissue Engineered Heart Valve by Using Combination of Antioxidant and Detergent: A Potential Therapy in Cardiovascular Tissue Engineering. Tissue Eng Regen Med 14, 755–762 (2017). https://doi.org/10.1007/s13770-017-0070-1
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DOI: https://doi.org/10.1007/s13770-017-0070-1