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Effects of Estradiol on the Paracrine Regulator Expression of In Vitro Maturated Murine Ovarian Follicles

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

The preservation of female germ cells is important in the individuals with ovarian dysfunction and failure. For this purpose, ovarian follicle in vitro maturation (OFIVM) is an important technology for the retrieval of mature oocytes. In the in vivo follicular development, paracrine factors such as angiotensin (AT) and anti-Müllerian hormone (AMH) play important roles. We attempted to add estrogen during the OFIVM and to assess their expression on the follicular cells. The ovaries and pre-antral follicles were collected from 13-day C57BL/6 mice and cultured in vitro with estradiol (E2) treatment for up to two weeks. In the whole ovaries, the expression of AT II was decreased and the expression of AMH was similar between control and E2-treated ovaries after in vitro culture. Although there was no difference in the survival, ovulation, maturation and fertilization rates between control and E2-treated groups, the expression of AT II in the follicular cells was down-regulated after E2 treatment at mRNA level, and AMH showed similar expression. In conclusion, adding E2 in OFIVM may regulate paracrine factors and their receptors that are related to follicular development. Further investigations are necessary to elucidate the roles of various sex hormones in the regulation of AT and AMH expression during the OFIVM.

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Acknowledgements

This research was funded by the grants of Ministry of Health and Welfare (HI14C2259) and Seoul National University Hospital (0420120210), Republic of Korea. The authors would like to express sincere thanks to the technical assistance of Soo Hyun Kim, Ye Seul Hwang and Kyung Mee Cho.

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    Authors and Affiliations

    1. Department of Obstetrics and Gynecology, Korea University Guro Hospital, Seoul, Korea

      Yong Jin Kim

    2. Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-gu, Seoul, 03080, Korea

      Kyung Eui Park, Yoon Young Kim, Hoon Kim, Seung-Yup Ku, Chang Suk Suh, Seok Hyun Kim & Young Min Choi

    3. Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, Korea

      Yoon Young Kim, Hoon Kim, Seung-Yup Ku, Chang Suk Suh, Seok Hyun Kim & Young Min Choi

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    1. Yong Jin Kim
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    Correspondence to Seung-Yup Ku.

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    The study was approved by the IACUC of the Seoul National University Hospital (IACUC no.15-0016-S1A0).

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    Yong Jin Kim and Kyung Eui Park contributed equally to this work.

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    Kim, Y.J., Park, K.E., Kim, Y.Y. et al. Effects of Estradiol on the Paracrine Regulator Expression of In Vitro Maturated Murine Ovarian Follicles. Tissue Eng Regen Med 14, 31–38 (2017). https://doi.org/10.1007/s13770-016-0006-1

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