Skip to main content

Advertisement

Log in

Characterization of a biodegrading bacterium, Bacillus subtilis, isolated from oil-contaminated soil

  • Original Paper
  • Published:
International Journal of Environmental Science and Technology Aims and scope Submit manuscript

Abstract

Crude oil and its derivatives because of different events and accidents may cause pollution to the environment. A biological treatment is a novel technique that uses microorganisms to remove or neutralize pollutants from a contaminated site. Oil-contaminated soils were sampled, after isolating of soil bacteria, using quantitative and qualitative screening, biosurfactant-producing bacteria were identified and environmental factors on the growth of bacteria and biosurfactant were investigated. In this study, the Bacillus subtilis was identified as the best biosurfactant-producing strain which has the ability to grow in environments with high salinity and temperature and pH > 5. The produced biosurfactant from B. subtilis is stable to changes in temperature and salt concentration and pH (in the range of 5–12).The B. subtilis also showed that they are able to biodegrade aliphatic alkanes. The B. subtilis has necessary potential for bioremediation of oil pollution in the environment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Abouseoud M, Maachi R, Amrane A, Boudergua S, Nabi A (2008) Evaluation of different carbon and nitrogen sources in production of biosurfactant by Pseudomonas fluorescens. Desalination 223(1):143–151

    Article  CAS  Google Scholar 

  • Bezza FA, Beukes M, Nkhalambayausi Chirwa EM (2015) Application of biosurfactant produced by Ochrobactrum intermedium CN3 for enhancing petroleum sludge bioremediation. Process Biochem 50(11):1911–1922

    Article  CAS  Google Scholar 

  • Bognolo G (1999) Biosurfactants as emulsifying agents for hydrocarbons. Colloids Surf A 152(1):41–52

    Article  CAS  Google Scholar 

  • Carrillo PG, Mardaraz C, Pitta-Alvarez SI, Giulietti AM (1996) Isolation and selection of biosurfactant-producing bacteria. World J Microbiol Biotechnol 12(1):82–84

    Article  CAS  Google Scholar 

  • Cavalero DA, Cooper DG (2003) The effect of medium composition on the structure and physical state of sophorolipids produced by Candida bombicola ATCC 22214. J Biotechnol 103(1):31–41

    Article  CAS  Google Scholar 

  • Cooper DG, Goldenberg B (1987) Surface active agents from two Bacillus species. Appl Environ Microbiol 53(2):224–229

    CAS  Google Scholar 

  • Fisher SH, Sonenshein AL (1991) control of carbon and nitrogen metabolism in Baciluss subtilus. Annu Rev Microbiol 45(1):107–135

    Article  CAS  Google Scholar 

  • Ilori MO, Amobi CJ, Odocha AC (2005) Factors affecting biosurfactant production by oil degrading Aeromonas spp. isolated from a tropical environment. Chemosphere 61(7):985–992

    Article  CAS  Google Scholar 

  • Jain RM, Mody K, Mishra A, Jha B (2012) Physicochemical characterization of biosurfactant and its potential to remove oil from soil and cotton cloth. Carbohydr Polym 89(4):1110–1116

    Article  CAS  Google Scholar 

  • Javaheri M, Jenneman GE, McInerney MJ, Knapp RM (1985) Anaerobic Production of a biosurfactant by Bacillus licheniformis JF-2. Appl Environ Microbiol 50(3):698–700

    CAS  Google Scholar 

  • Khopade A, Biao R, Liu X, Mahadik K, Zhang L, Kokare C (2012) Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4. Desalination 285:198–204

    Article  CAS  Google Scholar 

  • Makkar RS, Cameotra SS (1998) Production of biosurfactant at mesophilic and thermophilic conditions by a strain of B. subtilis. J Ind Microbiol Biotechnol 20(1):48–52

    Article  CAS  Google Scholar 

  • Margesin R (2000) Potential of cold-adapted microorganisms for bioremediation of oil-polluted Alpine soils. Int Biodeterior Biodegrad 46(1):3–10

    Article  CAS  Google Scholar 

  • Morikawa M, Daido H, Takao T, Murata S, Shimonishi Y, Imanaka T (1993) A new lipopeptide biosurfactant produced by Arthrobacter sp. strain MIS38. J Bacteriol 175(20):6459–6466

    Article  CAS  Google Scholar 

  • Nasr S, Soudi MR, Mehrnia MR, Sarrafzadeh MH (2009) Characterization of novel biosurfactant producing strains of Bacillus spp. isolated from petroleum contaminated soil. Iran J Microbiol 1(2):54–61

    Google Scholar 

  • Nitschke M, Pastore GM (2006) Production and properties of a surfactant obtained from B.subtilis grown on cassava wastewater. Bioresour Technol 97(2):336–341

    Article  CAS  Google Scholar 

  • Ojo OA (2006) Petroleum-hydrocarbon utilization by native bacterial population from a wastewater canal Southwest Nigeria. Afr J Biotechnol 5(4):333–337

    CAS  Google Scholar 

  • Rahman K, Street G (2002). Bioremediation sludge using bacterial consortium with biosurfactant. Clean Environ Manag-TS13BA, (Environmental Bioremediation Technologies (pp. 391–408). Springer Berlin)

  • Shahidi Rizi M, Akhavan Sepahi A, Sadat Tabatabaee M (2012) Crude oil biodegradation by a soil indigenous Bacillus sp. isolated from Lavan Island. Bioremediat J 16(4):218–224

    Article  Google Scholar 

  • Singh A, Van Hamme JD, Ward OP (2007) Surfactants in microbiology and biotechnology: part 2: application aspects. Biotechnol Adv 25(1):99–121

    Article  CAS  Google Scholar 

  • Tian Y, Fanb Y, Liub J, Zhaob X, Chen W (2016) Effect of nitrogen, carbon sources and agitation speed on acetoin production of B.subtilis SF4-3. Electron J Biotechnol 19:41–49

    Article  CAS  Google Scholar 

  • Trindade PVO, Sobral LG, Rizzo ACL, Leite SGF, Soriano AU (2005) Bioremediation of a weathered and a recently oil-contaminated soilsfrom Brazil: a comparison study. Chemosphere 58(4):515–522

    Article  CAS  Google Scholar 

  • Vasileva-Tonkova E, Galabova D (2003) Hydrolytic enzymes and surfactants of bacterial isolates from lubricant-contaminated wastewater. Z Naturforsch. C 58(1–2):87–92

    CAS  Google Scholar 

  • Verma S, Bhargava R, Pruthi V (2006) Oily sludge degradation by bacteria from Ankleshwar, India. Int Biodeterior Biodegrad 57(4):207–213

    Article  CAS  Google Scholar 

  • Xia WJ, Dong HP, Yu L, Yu DF (2011) Comparative study of biosurfactant produced by microorganisms isolated from formation water of petroleum reservoir. Colloids Surf A 392(1):124–130

    Article  CAS  Google Scholar 

  • Youssef NH, Duncan KE, Nagle DP, Savage KN, Knapp RM, McLnerney MJ (2004) Comparison of methods to detect biosurfactant production by diverse microorganisms. J Microbiol Methods 56(3):339–347

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors wish to sincerely thank all who assisted in preparation of this work.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Kh. Tabari.

Additional information

Editorial responsibility: M. Abbaspour.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tabari, K., Tabari, M. Characterization of a biodegrading bacterium, Bacillus subtilis, isolated from oil-contaminated soil. Int. J. Environ. Sci. Technol. 14, 2583–2590 (2017). https://doi.org/10.1007/s13762-017-1313-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13762-017-1313-3

Keywords

Navigation