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Exploring the interaction between “site-markers, aspirin and esterase-like activity” ternary systems on the human serum albumin: direct evidence for modulation of catalytic activity of the protein in different inhibition modes

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Abstract

Albumin which is the most abundant drug carrier protein in plasma controls the distribution aspect of drug pharmacokinetics. The aim of this study has been to elucidate the concurrent binding behavior of indomethacin/ibuprofen/heme to HSA under the effect of aspirin-mediated protein acetylation and also to explore the esterase-like catalytic property of the unmodified/modified proteins, as binary or ternary systems, by using various spectroscopic and molecular docking techniques. We found that aspirin-based modification of HSA affects the protein conformation as well as ligand binding at sites I–III. Decrease in pNPA-mediated esterase activity of HSA in different reversible inhibition modes, upon the protein–ligand interactions, was also documented, an issue that may receive considerable attention for computational prodrug design in near future.

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Acknowledgements

The authors gratefully acknowledge the Research Council of Kermanshah University of Medical Sciences, Kermanshah, Iran, for the financial supports (Grant No. 93102).

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Authors and Affiliations

  1. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Sajjad Esmaeili, Mohammad Reza Ashrafi-Kooshk & Reza Khodarahmi

  2. Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Masomeh Almasi & Asad Vaisi-Raygani

  3. Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Asad Vaisi-Raygani

  4. Nano Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Sajad Moradi

  5. Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mohsen Shahlaei

  6. Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Reza Khodarahmi

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  1. Sajjad Esmaeili
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  2. Masomeh Almasi
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Correspondence to Reza Khodarahmi.

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This work was performed in partial fulfillment of the requirements for M.Sc. degree of M. Almasi, in Department of Clinical Biochemistry and Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

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Esmaeili, S., Almasi, M., Vaisi-Raygani, A. et al. Exploring the interaction between “site-markers, aspirin and esterase-like activity” ternary systems on the human serum albumin: direct evidence for modulation of catalytic activity of the protein in different inhibition modes. J IRAN CHEM SOC 15, 555–573 (2018). https://doi.org/10.1007/s13738-017-1256-0

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