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A comparison of the inclusion behavior of human serum albumin and holo transferrin with fluoxymesterone in the presence of three different cyclodextrins

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Abstract

This article describes the interaction of fluoxymesterone (Flu) with HSA and HTF in the absence and presence of cyclodextrins (CDs) (α, β and γ). According to fluorescence data, the binding of Flu to the proteins caused strong static quenching in the binary and ternary systems. The fluorescence quenching results demonstrated that HSA and HTF had two and one class of apparent binding sites with a distinct binding constant in the presence of the CDs, respectively. The effects of Flu on the structure of HSA and HTF were analyzed using synchronous fluorescence spectroscopy, which showed that the interaction of Flu with both proteins in the binary and ternary systems altered the microenvironment around the Trp and Tyr residues. The distance, r, between Flu and the proteins was obtained according to FRET which pointed at a successful formation of a drug-protein complex. Far-UV CD spectra indicated that the binding of the drug to both proteins induced changes in the secondary structure of HSA and HTF in the binary and ternary systems. Finally, molecular modeling provided possible binding sites of Flu within the proteins for the binary and ternary systems and also confirmed the experimental results. The obtained data can be useful for determining usage drug doses in drug delivery.

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Acknowledgments

The financial support of the Research Council of the Mashhad Branch, Islamic Azad University, is gratefully acknowledged. The authors also thank Dr. Ljungberg for the English editing.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran

    Maryam Ghaderabad, Mahdieh Mansouri, Atena Sharifi Rad & Jamshidkhan Chamani

  2. Endoscopic and Minimally Invasive Surgery Research Center, Mashshad University of Medical Sciences, Mashhad, Iran

    Sima Beigoli

  3. Department of Biology, Faculty of Sciences, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran

    Jamshid Mehrzad

  4. Medical Chemistry Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Reza Saberi

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  1. Maryam Ghaderabad
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  2. Mahdieh Mansouri
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  4. Atena Sharifi Rad
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  5. Jamshid Mehrzad
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  7. Jamshidkhan Chamani
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Correspondence to Jamshidkhan Chamani.

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Ghaderabad, M., Mansouri, M., Beigoli, S. et al. A comparison of the inclusion behavior of human serum albumin and holo transferrin with fluoxymesterone in the presence of three different cyclodextrins. J IRAN CHEM SOC 14, 1347–1364 (2017). https://doi.org/10.1007/s13738-017-1085-1

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  • DOI: https://doi.org/10.1007/s13738-017-1085-1

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