Abstract
The levels of major bioactive compounds, such as amino acids and antioxidants, were investigated in okra sprouts and seeds of the varieties ‘Green Sword’ and ‘Benny’. Total polyphenol content was the highest in ‘Benny’ cotyledons, followed by ‘Benny’ hypocotyls and ‘Green Sword’ cotyledons. Polyphenol and flavonoid content in the two varieties were approximately 5- to 6-fold higher in sprouts than in seeds, and were higher in the cotyledons than in other tissues within the sprout. Activity of the radical scavenger DPPH was the highest in ‘Benny’ cotyledons, and mean values were 7.3-fold higher in sprouts than in seeds. Total amino acid content in both varieties was the highest in hypocotyls, followed by cotyledons and seeds. This content was about 40-fold higher in hypocotyls than in seeds. Alanine and Glycyrrhizin were the most abundant amino acids. Total vitamin C content was the highest in ‘Benny’ cotyledons, followed by ‘Green Sword’ cotyledons, and the lowest in hypocotyls of both varieties. In ‘Green Sword’ seeds, the levels of vitamins B1, B3, B5, and B6 were higher than the levels of B2 and B9. In ‘Benny’, B1 and B3 content were higher in the cotyledon, while B2, B5, and B9 content were higher in the hypocotyl. This study demonstrates the potential of okra sprouts to be used as a functional food ingredient and as a source of antioxidants.
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Literature Cited
Ahn YK, Jang KC, Kim SH (2013) Analysis of functional components in roasted okra (Abelmoschus esculentus L. Moench) seeds. Protected Hortic Plant Fac 22:61–64
Ahn YK, Kim SH, Seong KC, Moon DK (2011) Development of optimal pruning method on okra (Abelmoschus esculentus L. Moench) production. J Bio-Environ Control 20:58–61
AOAC (Association of Official Analytical Chemists) (1995) Official methods of analysis. 16th ed. Association of Official Analytical Chemists, Washington, DC, USA, pp 4:17, 32:21, 22, 32
Badshah A, Zed A, Sattar A (1991) Effect of soaking, germination and autoclaving on selected nutrients of rapeseed. Pak J Sci Ind Res 34:446–448
Bouché N, Fromm H (2004) GABA in plants: just a metabolite. Trends Plant Sci 9:110–115
Bown AW, Shelp BJ (1997) The metabolism and functions of y-aminobutyric acid. Plant Physiol 115:1–5
Cevallos-Casals BA, Cisneros-Zevallos L (2010) Impact of germination on phenolic content and antioxidant activity of 13 edible species. Food Chem 199:1485–1490
Cha MJ, Park EH, Kang SC, Baek KH (2011) Effects of various wavelength on the hardness and the free amino acid contents of soybean sprouts. Korean J Environ Agric 30:402–408
Chang JS, Lee BS, Kim YG (1992) Changes in γ-aminobutyric acid (GABA) and the mail constituents by a treatment conditions and of anaerobically treated green tea leaves. Korean J Food Sci Technol 24:315–319
Choi Y, Jeong HS, Lee J (2007) Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem 103:130–138
Crossley A, Hilditch TP (1951) The fatty acids and glycerides of okra seed oil. J Sci Food Agric 2:251–255
Dewanto V, Xianzhong W, Liu RH (2002) Processed sweet corn has higher antioxidant activity. J Agric Food Chem 50:4959–4964
Droge W (2001) Free radicals in the physiological control of cell function. Physiol Rev 82:47–95
El-Adawy TA (2002) Nutritional composition and antinutritional factors of chickpeas (Cicer arietinum L.) undergoing different cooking methods and germination. Plant Foods Hum Nutr 57: 83–97
Feng P (1997) A summary of background information and foodborne illness associated with the consumption of sprouts. Food and Drug Administration, Center for Food Safety and Applied Nutrition. http://www. cfsan.fda.gov/~mow/sprouts.html
Halliwell B, Aeschbach R, Loliger J, Aruoma OI (1995) The characterization of antioxidants. Food Chem Toxicol 33:601–607
Hasséssian H, Prat A, Champlain JD, Couture R (1991) Regulation of cardiovascular sympathetic neurons by substance P and γ-aminobutyric acid in the rat spinal cord. Eur J Pharmacol 202:51–60
Huang MT, Ho CT, Lee C (1992) Phenolic compounds in food and their effects on health (II): Antioxidants and cancer prevention. American Chemical Society, Washington, DC, USA, pp 54–71
Kἄhkὃnen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M (1999) Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 47:3954–3962
Kalueff A, Nutt DJ (1996) Role of GABA in memory and anxiety. Depress Anxiety 4:100–110
Khalil AW, Zed A, Mahmmod F, Tariq S, Khattak A.B, Shah H (2007) Comparison of sprout quality characteristics of desi and kabuli type chickpea culitivars (Cicer arietinum L.). Lebenson Wiss Technol 40:937–945
Kim EJ (2014) Physico-chemical and antioxidental property contrast of broccoli sprouts cultivated in different light sources. Department of Food Nutrition, The Gradute School Hoseo University Asan, Korea
Kim JC, Kim JD, Kim YS, Kim YS (1994) Varietal differences in morphological characters of bearing pod in okra (Abelmoschus esculentus L, Moench). J Korean Soc Int Agric 6:138–143
Kim JC, Lee JE, Son SY (1995) Studies on characters of bearing pod at flowering date, storage and cooking method of green pod in okra variety. J Korean Soc Int Agric 7:169–173
Kim JE, Joo SI, Seo JH, Lee SP (2009) Antioxidant and a-glucosidase inhibitory effect of tartary buckwheat extract obtained by the treatment of different solvents and enzymes. J Korean Soc Food Sci Nutr 38:989–995
Kim JE, Cho YS, Sung SK (2001) The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J Food Sci Technol 33:626–632
Kim KH (1992) The growing characteristics and proximate composition of soybean sprouts. Korean Soybean Dig 9:27–30
Kim SH, Kim JH, Lee HJ, Oh JM, Lee SH, Bahn KN, Seo IW, Lee YJ, Lee JH, et al. (2015) Simultaneous determination of water soluble vitamins B group in health functional foods etc. by HPLC. J Food Hyg Saf 30:143–149
Kim TS, Chang MS, Ju YW, Park CG, Park SI, Kang MH (2012) Nutritional evaluation of leafy safflower sprouts cultivated under different-colored lights. Korean J Food Sci Technol 44:224–227
Kinnersley AM, Turan FJ (2000) Gamma aminobutyric acid (GABA) and plant responses to stress. Crit Rev Plant Sci 19:479–509
Komatsuzaki N, Tsukahara K, Toyoshima H, Suzuki T, Shimizu N, Kimura T (2007) Effect of soaking and gaseous treatment on GABA content in germinated brown rice. J Food Eng 78:556–560
Kuo TM, VanMiddlesworth JF, Wolf WJ (1988) Content of raffinose and oligosaccharides and sucrose in various plants. J Agric Food Chem 36:32–36
Lamont W (1999) Okra -a versatile vegetable crop. Horttechnology 9:179–184
Lee EH, Kim CJ (2008) Nutritional changes of buckwheat during germination. J Korean Soc Food Cult 23:121–129
Lee MJ, Lim SY, Kim JK, Oh MM (2012) Heat shock treatments induce the accumulation of phytochemicals in Kale sprouts. Korean J Hortic Sci Technol 30:209–518
Lee MY, Shin SL, Park SH, Kim NR, Chang YD, LEE CH (2009) Development of optimal cultivation conditions and analysis of antioxidant activity of Arctium lappa sprout vegetables. Korean J Plant Res 22:304–311
Lee JJ, Lee YM, Shin HD, Jeong YS, Lee MY (2007) Effects of vegetable sprout power mixture on lipid metabolism in rats fed high fat diet. J Korean Soc Food Sci Nutr 36:965–974
Lee II, Lee BH, Eom SH, Oh CS, Kang HK, Cho YS, Kim DO (2015) Antioxidant capacity and protective effects on neuronal PC-12 cells of domestic bred kiwifruit. Korean J Hortic Sci Technol 33:259–267
Lee SM, Lee J (2006) Tocopherol and tocotrienol contents of vegetable oils, margarines, butters, and peanut butters consumed in Korean diet. Food Sci Biotechnol. 15:183–188
Lee YS, Kim YH (2004) Changes in postharvest respiration, growth and vitamin C content of soybean sprouts under different storage temperature conditions. Korean J Crop Sci 49:410–414
Leventhal AG, Wang YC, Pu M, Zhou Y, Ma Y (2003) GABA and its agonists improved visual cortical function in senescent monkeys. Science 300:812–815
Middleton E, Kandaswami C (1994) Potential health-promoting properties of citrus flavonoids. Food Technol 48:115–119
Nieva MM, Sampietro AR, Vattuone MA (2000) Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71:109–114
Olasantan FO, Bello NJ (2004) Optimum sowing dates for okra (Abelmoschus esculentus) in monoculture and mixture with cassava (Manihot esculenta) during the rainy season in the south-west of Nigeria. J Agric Sci 142:49–58
Park JH, Han SH, Shin MK, Park KH, Lim KC (2002) Effect of hypertention falling of functional GABA green tea. Korean J Med Crop Sci 10:37–40
Park KJ, Lim JH, Kim JH, Jeong JW, Jo JH, Jeong SW (2007) Reduction of microbial load on radish (Raphanus sativus L.) seeds by aqueous chlorine dioxide and hot water treatments. Korean J Food Preserv 14:487–491
Rice-Evans CA, Miller NJ, Paganga G (1997) Antioxidant properties of phenolic compounds. Trends Plant Sci 2:152–159
Sami R, Lianzhou J, Yang L, Ma Y, Jing J (2013) Evaluation of fatty acid and amino acid compositions in okra (Abelmoschus esculentus) grown in different geographical locations. Biomed Res Int 2013:1–6
Sami R, Li Y, Qi B, Wang S, Zhang Q, Han F, Ma Y, Jing J, Jiang L (2014) HPLC analysis of water-soluble vitamins (B2, B3, B6, B12, and C) and fat-soluble vitamins (E, K, D, A, and ß-Carotene) of Okra (Abelmoschus esculentus). J Chem 2014:1–6
Seo WD, Kim JY, Park DS, Han SI, Jang KC, Choi KJ, Kim SY, Oh SH, Ra JE, et al. (2011) Comparative analysis of physicochemicals and antioxidative properties of new giant embryo mutant, YR23517Acp79, in rice (Oryza sativa L.). J Korean Soc Appl Bio Chem 54:700–709
Soriano JM, Rico HM, Manes JC, Manes J (2000) Assessment of the microbiological quality and wash treatments of lettuce served in university restaurants. Int J Food Microbiol 58:123–128
Steward FC, Thompson JF, Dent C (1949) γ-aminobutyric acid: a constituent of potato tubers. Science 110:439–440
Thitinunsomboon S, Keeratipibul S, Boonsiriwit A (2013) Enhancing gamma-aminobutyric acid content in germinated brown rice by repeated treatment of soaking and incubation. Food Sci Technol Int 19:25–33
Tindall HD (1983) Vegetables in the tropics. McMillan AVI pp 325–327
Xu MJ, Dong JF, Zhu MY (2005) Effects of germination conditions on ascorbic acid level and yield of soybean sprouts. J Sci Food Agric 85:943–947
Yoon JE, Kim HS, Lee KA, Kim YH (2011) Contents of minerals and vitamines in soybean sprouts. Korean J Crop Sci 56:226–232
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Jeon, S.H., Cho, Y.S. & Rho, I.R. Evaluation of bioactive compounds in different tissues of sprouting okra. Hortic. Environ. Biotechnol. 58, 514–521 (2017). https://doi.org/10.1007/s13580-017-0261-7
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DOI: https://doi.org/10.1007/s13580-017-0261-7