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Enhanced drought and salt tolerance of Arabidopsis thaliana by ectopic expression of the molecular chaperone artemin from Artemia urmiana

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Abstract

Due to the resistance of Artemia urmiana to salt stress, researchers have isolated and investigated Artemin, the most prevalent protein within the cyst of this aquatic species. In vitro studies have revealed Artemin's role as a molecular chaperone, effectively engaging with the hydrophobic surfaces of unfolded and/or partially folded proteins. In light of Artemin's established functional significance, its encoding gene has been successfully introduced into mammalian cells; however, no published research has elucidated its potential role within plant cells. In the current investigation, the artemin gene was successfully cloned into the pPZPY122 plant vector and subsequently introduced into Arabidopsis thaliana plants. The T3 homozygote transgenic plants (art) were then subjected to a series of environmental stresses, including heat, salt (NaCl) and drought (Mannitol). To assess the mutant's resilience to these stresses, their seed germination indices were evaluated. The art line demonstrated a higher degree of tolerance towards the abiotic stresses. A comparative analysis revealed that ascorbate peroxidase activity, catalase activity, and proline content exhibited significantly enhanced levels in some NaCl-treated art plants compared to their counterparts in Col-0. Regarding the expression of the genes in the SOS pathway, it was found that SOS1 is significantly upregulated under NaCl treatment in the art mutant. Conversely, under normal growth conditions, the morphology and growth of transgenics remained indistinguishable from those of wild-type plants.

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Abbreviations

APX :

Ascorbate peroxidase

art :

Artemin lines

ART :

artemin Gene

CAT :

Catalase

Col-0:

Colombia-0

Csp:

Cold shock proteins

FGP :

Final germination percentage;

GB :

Glycine betaine

GI:

Germination index

Gm :

Gentamicin

GP :

Germination percentage

GR:

Germination rate,

MDG:

Mean daily germination,

MS :

Murashige and Skoog

P5CS :

Pyrroline-5-carboxylatesynthetase

PI4P :

Phosphatidylinositol 4-phosphate

sHSP :

Small heat shock proteins

SOD :

Superoxide dismutase

SOS :

Salt overly sensitive

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Acknowledgements

This work is based upon research funded by Iran National Science Foundation (INSF) under project number 95831962.

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  1. Zeinab Poormohammad and Sara Shahrokhi contributed equally to this work.

Authors and Affiliations

  1. Department of Biotechnology, College of Agricultural Sciences, University of Guilan, Rasht, 4199613769, Iran

    Zeinab Poormohammad, Sara Shahrokhi, Amin Abedi & M. Mehdi Sohani

  2. Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, P. O. Box: 14115-111, Tehran, Iran

    Reza H. Sajedi

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  1. Zeinab Poormohammad
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Poormohammad, Z., Shahrokhi, S., Abedi, A. et al. Enhanced drought and salt tolerance of Arabidopsis thaliana by ectopic expression of the molecular chaperone artemin from Artemia urmiana. J. Plant Biochem. Biotechnol. (2024). https://doi.org/10.1007/s13562-024-00877-1

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