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Potato peels for tannase production from Penicillium commune HS2, a high tannin-tolerant strain, and its optimization using response surface methodology

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Abstract

Tannase catalyzes the de-esterification of tannins into gallic acid and glucose. This enzyme has extensive value in removing tannins from tea, beer, and wine. For its benefit, innovative tannase-producing organisms continue to be reported in the literature. In this study, a novel tannase-producing fungal strain with a high tolerance to tannin was isolated from corn cobs and identified as Penicillium commune HS2. Four variables, i.e., initial pH, temperature, potato peel, and tannin concentrations, were evaluated to optimize their effects on tannase production. Using central composite design (CCD) of response surface methodology (RSM) for the optimization of tannase production on potato peels, a 4.62-fold upsurge was successfully achieved. The maximum productivity of 288.48 U from 1 g of dry potato peels was obtained under solid-state fermentation (SSF) at pH 5.0, 25.1 °C, in a medium containing 1.13% and 9.99% of potato peel and tannin, respectively. The purified enzyme had a molecular weight (Mw) of 35 kDa and showed maximal activity at 40–50 °C and a pH range of 4–5, as well as a half-life of 70 min at 40 °C. Using the tannic acid as a substrate, the enzyme had a Km value of 0.217 mM and Vmax of 8.08 U/ml/min. The purified enzyme successfully reduced 33.89% of total tannin content in lemon tea after 2 h at 45 °C. It can be concluded that Penicillium commune is a potentially high-tolerant tannin fungus that may be produced commercially on potato peel waste at a low cost and has promising applications in the food sector.

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Acknowledgements

The author thanks Dr. Heba Mohamad for the assistance in phylogenetic tree drawing.

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  1. Food Science Department, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt

    Heba Sayed Mostafa

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The original online version of this article was revised: During the production process, the arrangement of Table 3 columns are wrong.

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Mostafa, H.S. Potato peels for tannase production from Penicillium commune HS2, a high tannin-tolerant strain, and its optimization using response surface methodology. Biomass Conv. Bioref. 13, 16765–16778 (2023). https://doi.org/10.1007/s13399-021-02205-2

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