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Using mini-xylanosomes as a biotechnological tool for sisal fiber deconstruction and enzyme immobilization

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Abstract

Currently, there is an increasing demand for developing enzymatic blends that can efficiently deconstruct the hemicellulose present in plant fibers, since these fibers can be further employed to build new engineered materials. Sisal fibers are investigated as a source of cellulosic material to manufacture new materials, as reinforced fiber with plastic additives. A critical step to obtain the cellulosic matrix is the removal of hemicellulose present on these fibers. In the present study, a mini-xylanosome was tailored using a miniature version of C. thermocellum’s scaffolding protein (two cohesins I domains and one carbohydrate-binding module family 3) and the xylanase XynZ. The designed xylanosome displayed higher thermal stability at 70 °C in comparison to the free form XynZ, 134 and 90 min of half-lives, respectively. Complexing xylanases into mini-xylanosomes or immobilizing complexes into cellulose did not reduce activity loss by tannic acid. Regarding sisal fiber deconstruction, the mini-xylanosome displayed better hydrolyzing capacity than the non-complexed xylanase, resulting in three times higher reducing sugars. Immobilized mini-xylanosome could be subjected to 72 h of reuse cycles at 50 and 60 °C, and at 70 °C for 48 h.

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Acknowledgements

Pedro R.V Hamann is a recipient of CAPES doctoral scholarship, Tainah C. Gomes, and Luísa de M.B Silva are recipients of CAPES master degree scholarship. Eliane F. Noronha is a research fellow of CNPq. This research was supported by grants from the University of Brasília and FAP-DF.

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  1. Cell Biology Department, Enzymology Laboratory, University of Brasilia, Brasilia, Brazil

    Pedro R. V. Hamann, Tainah C. Gomes, Luísa de M. B. Silva & Eliane F. Noronha

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  1. Pedro R. V. Hamann
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  2. Tainah C. Gomes
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  3. Luísa de M. B. Silva
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  4. Eliane F. Noronha
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Pedro R.V Hamann wrote the manuscript and conceived experiments; Tainah C. Gomes conceived experiments; Luísa de M.B Silva conceived experiments; Eliane F. Noronha revised the manuscript, conceived experiments, and was responsible for funding acquisition.

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Correspondence to Pedro R. V. Hamann.

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The manuscript was read and approved by all authors; the authors would like to declare that there is no conflict of interest, and credit has appropriately been given to everyone who contributed to the present study.

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Highlights

• Hydrolysis of sisal fibers using mini-xylanosomes generates three times more reducing sugar than non-complexed xylanase.

• Cellulose immobilized mini-xylanosomes could be subject to 72 h of reuse cycles at 50 and 60 °C.

• At 70 °C, xylanases assembled into mini-xylanosomes display better thermal stability than non-complexed.

• Complexing the xylanase into mini-xylanosomes does not change the hydrolysis yield of soluble oat spelt xylan.

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Hamann, P.R.V., Gomes, T.C., Silva, L.d.M.B. et al. Using mini-xylanosomes as a biotechnological tool for sisal fiber deconstruction and enzyme immobilization. Biomass Conv. Bioref. 13, 12143–12155 (2023). https://doi.org/10.1007/s13399-021-02137-x

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