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Isolation of cellulolytic microcosms from bagasse compost in co-digested fibrous substrates

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Abstract

Symbiotic cellulolytic microcosms represent a promising microbial agent for enhancing degradation of cellulosic materials in biotechnological processes. In this study, structurally stable lignocellulose-degrading microbial communities were constructed from cellulolytic seed culture from sugarcane bagasse compost using swine manure and Napier grass as co-digested carbon sources under static aerobic condition at 55 °C. The lignocellulolytic microbial consortium enriched in peptone and yeast extract-based medium (PLMC) showed higher cellulose-degrading activity compared to lignocellulolytic microbial consortium isolated in the water-based medium (WLMC). The composite microbes in both consortia were originated from the seed culture and the co-digested substrates according to denaturing gradient gel electrophoresis profile. PLMC exhibited higher CMCase, xylanase, FPase, and avicelase activities in the supernatant than those of WLMC and was capable of degrading 70 % of filter paper within 1 week. PLMC was capable of degrading substrate with higher efficiency than the control by 11.7, 9.2, 15.5, and 11.9 % of total solid, suspended solid, volatile solid, and volatile suspended solid, respectively. The work demonstrated the potential of cellulolytic microcosms enriched by this approach on enhancing conversion efficiency in biogas production from cellulosic wastes.

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Acknowledgments

This study was financially supported by The Joint Graduate School of Energy and Environment (JGSEE) at King Mongkut’s University of Technology Thonburi (KMUTT).

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Authors and Affiliations

  1. The Joint Graduate School of Energy and Environment, King Mongkut’s University of Technology Thonburi, Bangkok, 10140, Thailand

    Sarunyou Wongwilaiwalin

  2. Enzyme Technology Laboratory, National Center for Genetic Engineering and Biotechnology, Thailand Science Park, Pathumthani, 12120, Thailand

    Wuttichai Mhuantong & Verawat Champreda

  3. Genome Institute, National Center for Genetic Engineering and Biotechnology, Thailand Science Park, Pathumthani, 12120, Thailand

    Sithichoke Tangphatsornruang

  4. Excellent Center of Waste Utilization and Management, National Center for Genetic Engineering and Biotechnology at King Mongkut’s University of Technology Thonburi, Bangkok, 10150, Thailand

    Pornpan Panichnumsin

  5. Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok, 10150, Thailand

    Chakrit Tachaapaikoon

Authors
  1. Sarunyou Wongwilaiwalin
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  2. Wuttichai Mhuantong
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  3. Sithichoke Tangphatsornruang
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  4. Pornpan Panichnumsin
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  5. Verawat Champreda
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  6. Chakrit Tachaapaikoon
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Correspondence to Chakrit Tachaapaikoon.

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Wongwilaiwalin, S., Mhuantong, W., Tangphatsornruang, S. et al. Isolation of cellulolytic microcosms from bagasse compost in co-digested fibrous substrates. Biomass Conv. Bioref. 6, 421–426 (2016). https://doi.org/10.1007/s13399-016-0199-5

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  • DOI: https://doi.org/10.1007/s13399-016-0199-5

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