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In vitro evaluation of biofilm phenotypic and genotypic characteristics among clinical isolates of Pseudomonas aeruginosa in Hamadan, West of Iran

  • Microbial Genetics • Original Paper
  • Published:
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Abstract

Due to high antimicrobial resistance and biofilm-forming ability, Pseudomonas aeruginosa is one of the seriously life-threatening agents causing chronic and nosocomial infections. This study was performed to determine the antibiotic resistance pattern, biofilm formation, and frequency of biofilm-related genes in P. aeruginosa strains. In total, 123 P. aeruginosa isolates were collected from different clinical sources. Antimicrobial susceptibility testing (AST) was performed to detect multidrug-resistant P. aeruginosa (MDRPA) isolates. To evaluate the biofilm-forming isolates, the microtiter plate (MTP) method was carried out. Also, the prevalence of biofilm genotype patterns, including pslA, pslD, pelA, pelF, and algD genes, was detected by polymerases chain reaction (PCR). According to our findings, the highest resistance and susceptibility rates were found in ceftazidime with 74.7% (n = 92) and ciprofloxacin with 42.2% (n = 52), respectively. In our study, the highest level of antibiotic resistance belonged to wound isolates which meropenem had the most antibacterial activity against them. In total, 86.1% (n = 106) P. aeruginosa isolates were determined as MDRPA, of which 61.3% (n = 65) were able to form strong biofilm. The highest and lowest frequency of biofilm-related genes among biofilm producer isolates belonged to pelF with 82.1% (n = 101) and algD with 55.2% (n = 68), respectively. The findings of the conducted study indicate a significant relationship between MDRPA and biofilm genotypic/phenotypic patterns, suggesting the necessity of a careful surveillance program in hospital settings.

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Due to the nature of this research, participants of this study did not agree for their data to be shared publicly, so supporting data is not available.

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Acknowledgements

The authors would like to acknowledge the Vice-Chancellor of Hamadan University of Medical Sciences for the support of the study.

Funding

This study was funded by Hamadan University of Medical Sciences, Hamadan, Iran (Project No. 140107266229).

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Authors and Affiliations

  1. Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Jaber Hemmati, Mohsen Nazari, Amjad Ahmadi, Maral Bayati, Mahsa Jalili, Mohammad Taheri & Babak Asghari

  2. Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran

    Jaber Hemmati & Mohsen Nazari

  3. Department of Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran

    Younes Mohammadi

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  1. Jaber Hemmati
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  2. Mohsen Nazari
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  5. Mahsa Jalili
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  6. Mohammad Taheri
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Contributions

B. A and M. T designed the study. M. B, M. N, J. H, and A. A contributed to the experimental studies and Y. M analyzed the data. J. H, A. A, M. N, and M. J contributed to the sample collections. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Babak Asghari.

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Conflict of interest

There are no contradictions or interests among the authors.

Ethics approval

This study was approved by the Ethics Committee of Hamadan University of Medical Sciences, Hamadan, Iran (Ethical approval No.IR.UMSHA.REC.1401.579).

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Communicated by: Agnieszka Szalewska-Palasz

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Hemmati, J., Nazari, M., Ahmadi, A. et al. In vitro evaluation of biofilm phenotypic and genotypic characteristics among clinical isolates of Pseudomonas aeruginosa in Hamadan, West of Iran. J Appl Genetics 65, 213–222 (2024). https://doi.org/10.1007/s13353-023-00811-6

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