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Development of doxorubicin-encapsulated magnetic liposome@PEG for treatment of breast cancer in BALB/c mice

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Abstract

The magnetic doxorubicin-encapsulated liposome/PEG/Fe3O4 (called as DOX@m-Lip/PEG) was synthesized and studied as a novel nanocarrier for the treatment of breast cancer in BALB/c mice. Nanocarrier was characterized by FT-IR, zeta-potential sizer, EDX elemental analysis, EDX mapping, TEM, and DLS techniques. The results showed that the size of the nanocarrier was determined around 128 nm by TEM. EDX study confirmed PEG-conjugation in the magnetic liposomes and was homogenously distributed in the nanosize range (100–200 nm) with a negative surface charge (−61.7 mV). The kinetic studies indicated that the release of doxorubicin from DOX@m-Lip/PEG follows the Korsmeyer-Peppas model. The n-value of the model was 0.315, indicating that doxorubicin release from the nanocarrier had a slow releasing rate and followed Fick’s law. The DOX release from the nanocarrier lasted a long time (more than 300 h). In in vivo part, a mouse 4T1 breast tumor model was used. The in vivo results indicated that DOX@m-Lip/PEG caused much stronger tumor cell necrosis and less cardiotoxic effects than the other groups. In conclusion, we show that m-Lip/PEG is a promising nanocarrier for low dosage and slow release of doxorubicin in treating breast cancer, and treatment with encapsulated DOX (DOX@m-Lip/PEG) demonstrated higher efficacy with low cardiac toxicity. Besides, the magnetic property of m-Lip@PEG nanocarrier allows it to be a potent mater for hyperthermia and MRI studies.

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Abbreviations

DOX:

Doxorubicin

NPs:

Nanoparticles

PBS:

Phosphate-buffered saline

DDS:

Drug delivery system

PEG 2000:

Polyethylene glycol 2000

Fe3O4 :

Magnetite

LNPs:

Lipid nanoparticles

PI:

Polydispersity index

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Acknowledgements

This work was fulfilled as MSc thesis work at Urmia University. The authors appreciate the Research Deputy of Urmia University. We would like to thank Dr. Ali Asghar Tehrani (Pathologist, Faculty of Veterinary Medicine, Urmia University) for her valuable guidance regarding the interpretation of histopathological analysis.

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Authors and Affiliations

  1. Department of Applied Chemistry, Faculty of Chemistry, Urmia University, Urmia, Iran

    Sajjad Maghsoudi & Seyed Ali Hosseini

  2. Department of Pharmacology and Toxicology, School of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran

    Hamid Soraya

  3. Hematology, Immune Cell Therapy and Stem Cells Transplantation Research Center, Clinical Research Institute, Urmia University of Medical Sciences, Urmia, Iran

    Yousef Roosta

  4. Department of Immunology and Genetics, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

    Adel Mohammadzadeh

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  1. Sajjad Maghsoudi
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  2. Seyed Ali Hosseini
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  3. Hamid Soraya
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  4. Yousef Roosta
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  5. Adel Mohammadzadeh
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Contributions

Sajjad Maghsoudi did the experiments and characterizations. He prepared the draft of the manuscript. Seyed Ali Hosseini and Hamid Soraya were the supervisors of the thesis. They edited the manuscript. Yousef Roosta and Adel Mohammadzadeh were the advisors of the thesis and guided cell culture and cancer tests. They also read the manuscript and approved it.

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Correspondence to Seyed Ali Hosseini.

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This work has two ethics approval from Urmia University and Urmia University of Medical Sciences with ID of IR-UU-AEC-1504/PD/3 and IR-IMSU.AEC.1400.002, respectively. They are presented as supplementary data.

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Maghsoudi, S., Hosseini, S.A., Soraya, H. et al. Development of doxorubicin-encapsulated magnetic liposome@PEG for treatment of breast cancer in BALB/c mice. Drug Deliv. and Transl. Res. 13, 2589–2603 (2023). https://doi.org/10.1007/s13346-023-01339-2

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  • DOI: https://doi.org/10.1007/s13346-023-01339-2

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