Abstract
In the present work, it was phenotyped 29 Coffea canephora clones for resistance to Hemileia vastatrix races II and XXXIII and investigated the presence of molecular markers associated with Coffee Leaf Rust (CLR) and Coffee Berry Disease (CBD) resistance loci in 67 clones. Phenotyping for resistance was performed by evaluating six components of quantitative resistance using the leaf disc technique. The averages of the components were submitted to the Scott-Knott test and to the multivariate analysis to obtain the generalized Mahalanobis distance, which was used to group the clones by the unweighted pair-group method using arithmetic averages. In screening resistance to CLR, molecular markers were evaluated associated with two resistance loci. Three sequence characterized amplified regions (SCAR) and a simple sequence repeats (SSR) markers associated with the locus of SH3 gene (Locus A); a SCAR and a cleaved amplified polymorphic site (CAPS) associated with a locus of resistance to races I, II and pathotype 001 of H. vastatrix (Locus B). For screening resistance to CBD, two SSRs were associated with the locus of the resistance Ck-1 gene (Locus C) were evaluated. The phenotyped clones were grouped into five resistance classes for each H. vastatrix race. Fifteen clones have locus of resistance to races I, II, and the pathotype 001 of H. vastatrix. Three clones have the Ck-1 gene. These results support growers to choose resistant clones for crop composition and indicate potential sources of CLR and CBD resistance for C. canephora breeding programs.
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The data collected during the performance of this work may be made available by the corresponding author upon a reasonable request.
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Acknowledgements
We would like to thank the National Council for Scientific and Technological Development (CNPq) for granting a scholarship to R. V. Gonzales for the development of this work. We also thank the Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural and nurseries from the states of Espírito Santo and Bahia who kindly provided seedlings of the clones evaluated in this work.
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Authors contributed to the conception, choice of methodologies and design of the work. The author Abraão Carlos Verdin-Filho helped in choosing the population and obtaining seedlings and leaves of the respective clones. Author Rafael Vago Gonzales conducted the phenotyping trials. The authors Rafael Vago Gonzales and Dênia Pires de Almeida conducted the screening of resistance loci. Laércio Zambolim assisted in the interpretation of phenotypic data and Eveline Teixeira Caixeta assisted in the interpretation of molecular data. The author Renato Domiciano Silva Rosado assisted in the statistical analysis of the phenotypic data.
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Gonzales, R.V., Zambolim, L., Almeida, D.P. et al. Prospects for achieving multiple disease resistance with Coffea canephora clones. Australasian Plant Pathol. 52, 451–462 (2023). https://doi.org/10.1007/s13313-023-00933-9
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DOI: https://doi.org/10.1007/s13313-023-00933-9