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Thiamine induces resistance in tobacco against black shank

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Abstract

Induced resistance by elicitors is considered to be an eco-friendly strategy to stimulate plant defence against pathogen attack. Thiamine (vitamin B1, VB1) can act as a plant defence trigger or priming agent, leading to a rapid counterattack on pathogen invasion, but the underlying molecular mechanisms have not yet been fully elucidated. In the present study, the priming effect of thiamine on tobacco against the disease Phytophthora nicotianae and its biochemical and molecular impact on plant defence mechanisms, as well as the in vitro inhibitory effect of thiamine on P. nicotianae, were evaluated. The results showed that the mycelial growth and sporangium production of P. nicotianae were inhibited by thiamine in a dose-dependent manner. After thiamine pretreatment, the resistance of tobacco plants to P. nicotianae was enhanced, and the severity of tobacco related disease was significantly reduced. In tobacco plants stimulated by thiamine, H2O2 accumulation and catalase (CAT) and peroxidase (POD) and phenylalanine ammonia lyase (PAL) activity levels were enhanced, and seven defence-related genes were upregulated in the plant leaves in order to avoid anthropomorphising plant responses to pathogen attack. Overall, this study demonstrates that thiamine effectively induces resistance against P. nicotianae in tobacco under greenhouse-controlled conditions through a dual mode of action involving direct antifungal activity and induction of host defence mechanisms. It is suggested that thiamine may be an attractive alternative to chemical fungicides in tobacco plant disease management.

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Acknowledgements

This work was supported by Yunnan Tobacco Company Science and Technology Plan Project (Number: 2020530000242026).

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Author notes

  1. Liu Jun and Liu Tao equally contributed to this work

Authors and Affiliations

  1. College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China

    Tian Suohui, Chen Yanping, Zi Shuhui & Liu Tao

  2. Technology Centre of Hongyun Honghe Tobacco Group, Kunming, 650201, Yunnan, China

    Li Zhihua, Jin Honggang & Liu Jun

Authors
  1. Tian Suohui
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  2. Chen Yanping
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  3. Zi Shuhui
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  4. Li Zhihua
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  5. Jin Honggang
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  6. Liu Jun
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  7. Liu Tao
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Contributions

Tao Liu and Jun Liu conceived and designed the experiments. Tian Suohui and Chen Yanping performed the experiments. Zi Shuhui and Li Zhihua analysed the data. Jin Honggang revised the paper. All authors read and approved the final manuscript.

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Correspondence to Tian Suohui or Liu Tao.

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Suohui, T., Yanping, C., Shuhui, Z. et al. Thiamine induces resistance in tobacco against black shank. Australasian Plant Pathol. 51, 231–243 (2022). https://doi.org/10.1007/s13313-021-00848-3

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