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Prognostic value of mixed lineage kinase domain-like protein expression in the survival of patients with gastric caner

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Tumor Biology

Abstract

The aim of this study was to detect mixed lineage kinase domain-like protein (MLKL) expression in gastric cancer (GC) and to analyze its association with the prognosis of GC patients. Immunohistochemical staining, Western blotting, and quantitative reverse-transcriptase polymerase chain reaction were performed to detect MLKL tissue expression in 117 GC patients. Clinicopathological characteristics and survival data were retrospectively analyzed to discover the clinical importance of MLKL expression. The chi-square test was used to analyze the relationship between MLKL expression and the clinicopathological characteristics. Survival curves were plotted by using the Kaplan–Meier method and compared using the log-rank test. Survival data were evaluated using univariate and multivariate Cox regression analyses. The expression of MLKL mRNA was significantly higher in adjacent normal samples than in the tumor tissues (P = 0.003). Clinicopathological analysis showed that MLKL expression was significantly correlated with age (P = 0.013), histologic type (P = 0.049), differentiation grade (P < 0.001), depth of invasion (P = 0.022), and lymph node metastasis (P = 0.003). Low MLKL expression was significantly associated with decreased overall survival (median 29 months vs. 56 months, P < 0.001). Multivariate analysis suggested that MLKL expression might be an independent prognostic indicator (HR = 0.645, 95 % CI, 0.446–1.165, P = 0.002) for GC patients. In conclusion, our findings provide evidence that MLKL might serve as a candidate tumor suppressor and a potential prognostic biomarker for GC.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grant Nos. 81372341 and 30972883) and “3 & 3” project of The First Affiliated Hospital of Sun Yat-sen University (He Yulong).

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    Authors and Affiliations

    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan 2nd road, Guangzhou, 510080, China

      Zhai Ertao, Chen Jianhui, Wang Kang, Ye Zhijun, Wu Hui, Chen Chuangqi, Qin Changjiang, Chen Sile, He Yulong & Cai Shirong

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    1. Zhai Ertao
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    2. Chen Jianhui
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    3. Wang Kang
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    4. Ye Zhijun
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    5. Wu Hui
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    6. Chen Chuangqi
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    7. Qin Changjiang
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    8. Chen Sile
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    9. He Yulong
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    Correspondence to Wu Hui or Cai Shirong.

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    All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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    Zhai Ertao and Chen Jianhui contributed equally to this work.

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    Ertao, Z., Jianhui, C., Kang, W. et al. Prognostic value of mixed lineage kinase domain-like protein expression in the survival of patients with gastric caner. Tumor Biol. 37, 13679–13685 (2016). https://doi.org/10.1007/s13277-016-5229-1

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    • DOI: https://doi.org/10.1007/s13277-016-5229-1

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