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Effect of Shenfu injection on immune function of mice bearing Lewis lung sarcoma with chemotherapy

  • Original Article
  • Published:
Tumor Biology

Abstract

Chemotherapy damages immune system and has a lot of side effects. In this study, we investigate the function of Shenfu injection to reduce these unfavorable effects induced by chemotherapy on mice bearing Lewis lung sarcoma. Mice inoculated with Lewis lung sarcoma cells were divided into five groups: Lewis lung sarcoma control group, cyclophosphamide (CTX) group, and Shenfu injection (high, moderate, and low dose) + CTX group. After a 14-day treatment, the counting of peripheral blood cells, CD3+, CD4+, and CD8+ T lymphocytes were done, immunoglobulin (Ig) was measured, coefficients of spleen and thymus were calculated, and spleen T cell proliferation was evaluated in vitro. The CD4+/CD8+ and CD3+ T cells in high- and moderate-dose Shenfu groups were more than the CTX group (p < 0.05); spleen T cell proliferation of mice in high-dose Shenfu + CTX group is more prominent than the CTX group (p < 0.05); coefficients of spleen and thymus, WBC, and platelet (PLT) counting of mice in the CTX group were lower than control and high and moderate dose Shenfu + CTX groups. The level of serous IgG and IgM of all test groups shows no significant difference. Shenfu injection can improve cellular immune function and reduce myolosuppression of mice delivered with chemotherapy.

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Authors and Affiliations

  1. Department of Radiation Oncology, Sichuan Cancer Hospital and Institute, Chengdu, Sichuan, 610041, China

    Yukun Luo & Jinyi Lang

  2. Department of Thoracic Surgery, Sichuan Cancer Hospital and Institute, No.55 Section 4 of Renmin South Rd. Wuhou, Chengdu, Sichuan, 610041, China

    Kangning Wang & Qiang Li

Authors
  1. Yukun Luo
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  2. Kangning Wang
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  3. Qiang Li
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  4. Jinyi Lang
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Corresponding author

Correspondence to Kangning Wang.

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All experiments were carried out in accordance with the institutional guidelines, and the study protocol was approved by the Institutional Animal Care and Use Committee (IACUC) at the Sichuan Cancer Hospital.

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None.

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Luo, Y., Wang, K., Li, Q. et al. Effect of Shenfu injection on immune function of mice bearing Lewis lung sarcoma with chemotherapy. Tumor Biol. 37, 10187–10191 (2016). https://doi.org/10.1007/s13277-016-4889-1

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  • DOI: https://doi.org/10.1007/s13277-016-4889-1

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