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RETRACTED ARTICLE: MicroRNA-32 inhibits osteosarcoma cell proliferation and invasion by targeting Sox9

  • Research Article
  • Published:
Tumor Biology

This article was retracted on 20 April 2017

Abstract

Increasing reports suggest that discovery of microRNAs (miRNAs) might provide a novel therapeutical target for human cancers, including osteosarcoma. Previous studies have shown that miR-32 was dysregulated in breast and endometrial cancer. However, its biological roles in osteosarcoma remain unclear. In the current study, we found that miR-32 was significantly down-regulated in osteosarcoma tissues, compared with the adjacent normal tissues. In vitro studies further demonstrated that miR-32 mimics were able to suppress, while its antisense oligos promoted cell proliferation in Saos-2 and U2OS cells. At the molecular level, our data further revealed that expression of Sox9 was negatively regulated by miR-32. Therefore, our results identify an important role for miR-32 in the osteosarcoma through regulating Sox9 expression.

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Authors and Affiliations

  1. Department of Orthopaedics, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, No.197, Ruijin 2nd Road, Shanghai, 200025, China

    Jian-Qiang Xu, Wei-Bin Zhang, Rong Wan & Yao-Qi Yang

Authors
  1. Jian-Qiang Xu
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  2. Wei-Bin Zhang
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  3. Rong Wan
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  4. Yao-Qi Yang
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Corresponding author

Correspondence to Wei-Bin Zhang.

Additional information

The Publisher and Editor retract this article in accordance with the recommendations of the Committee on Publication Ethics (COPE). After a thorough investigation we have strong reason to believe that the peer review process was compromised.

An erratum to this article is available at http://dx.doi.org/10.1007/s13277-017-5487-6.

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Supplementary Fig. 1

Expression levels of PTEN in Saos-2 and U2OS cells. (A-B) Protein levels of PTEN were determined by western blot in Saos-2 (A) and U2OS (B) cells transfected with miR-32 mimics or negative controls (NC). (JPEG 81 kb)

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Supplementary Fig. 2

Sox9 re-introduction blocks the anti-proliferative roles of miR-32 in U2OS cells. (A) Sox9 protein expression was determined by western blot in U2OS cells. Cells were pre-transfected with miR-32 mimics or negative control (NC) for 24 hr, and then transfected with lentiviruses containing Sox9 gene or empty vector (EV) for another 24 hr. (B-D) The growth curve (B), cell proliferation (BrdU, C) and invasion abilities (D) was determined in U2OS cells. *P < 0.05 **P < 0.01, ***P < 0.001 between two groups. (JPEG 107 kb)

High resolution image (TIFF 435 kb)

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Xu, JQ., Zhang, WB., Wan, R. et al. RETRACTED ARTICLE: MicroRNA-32 inhibits osteosarcoma cell proliferation and invasion by targeting Sox9. Tumor Biol. 35, 9847–9853 (2014). https://doi.org/10.1007/s13277-014-2229-x

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  • DOI: https://doi.org/10.1007/s13277-014-2229-x

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