Abstract
Background
Hashimoto's thyroiditis (HT), also known as chronic lymphocytic thyroiditis, is one of the most common autoimmune disease (AITD) in clinical practice. It is urgent to explore the mechanism of amiodarone-induced thyroid dysfunction.
Objective
This study aims to assess the expression levels of miR-214 and FasL in amiodarone contact type of HT, and the effect of miR-214 on cell viability and apoptosis and potential mechanism.
Results
We found that miR-214 was low expressed in the tissues of amiodarone-treated thyroiditis patients. MiR-214 increased the survival rate of amiodarone-induced thyroid epithelial follicular cells and inhibited apoptosis. Mechanically, we found that miR-214 could bind to FASL and regulate MAPK signaling pathway through FASL.
Conclusions
Our results suggested that miR-214 could be a potential therapeutic target for Hashimoto's thyroiditis.
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Funding
This work was supported by the National Natural Science Foundation of China (Grant No. 81760141), Guizhou Provincial Project of Science and Technology Foundation (Grant No. 20161122), Academic Newseedling Project of Guizhou Medical University (Grant No. 19NSP053), Doctor Initiation Fund Project of the Affiliated Hospital of Guizhou Medical University (Grant No. BSH-Q-2020-09), Science and Technology Fund Project of Guizhou Health Commission (Grant No. gzwkj2021-376), and Science and Technology Fund Project of Guizhou Health Commission (Grant No. gzwkj2021-381).
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JW and CD designed the study, supervised the data collection, LS and SZ analyzed the data, interpreted the data, MZ, XH, NW and LL prepare the manuscript for publication and reviewed the draft of the manuscript. All authors have read and approved the manuscript.
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Jing Wen declares that she has no conflict of interest. Chaonan Deng declares that she has no conflict of interest. Lixin Shi declares that he has no conflict of interest. Shi Zhou declares that he has no conflict of interest. Miao Zhang declares that she has no conflict of interest. Xiaoli Hu declares that she has no conflict of interest. Nianxue Wang declares that she has no conflict of interest. Lijuan Luo declares that she has no conflict of interest.
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Ethical approval was obtained from the Ethics Committee of the Affiliated Hospital of Guizhou Medical University (Approval No. 2017-037).
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Wen, J., Deng, C., Shi, L. et al. MiR-214 inhibits apoptosis in thyroid epithelial follicular cells induced by amiodarone through the FASL/MAPK pathway. Mol. Cell. Toxicol. 18, 225–232 (2022). https://doi.org/10.1007/s13273-021-00192-z
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DOI: https://doi.org/10.1007/s13273-021-00192-z