Abstract
Tumor necrosis factor-α (TNF-α) plays an important role in inflammatory responses. Deregulated activation of TNF-α signaling is associated with various inflammatory diseases. In view of the finding that the seventy percent ethanol extract of Litsea japonica (LJE) interferes with TNF-α-induced NF-κB activation, activity- guided fractionation was performed to identify the active components of the extract. The EtOAc, CH2Cl2 and n-Hexane fractions of LJE induced suppression of inhibitor of κB (IκB) kinase (IKK), p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK), and significant reduction of TNF-α- induced NF-κB activation. From the CH2Cl2 fraction, hamabiwalactone A, hamabiwalactone B, litsenolide B2 and litsenolide C2 were identified to inhibit TNF- α-induced NF-κB activation. The cytotoxic activities of these lactone compounds were additionally evaluated.
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References
Hayden, M. S. & Ghosh, S. Regulation of NF-kappaB by TNF family cytokines. Semin Immunol 26:253–266 (2014).
Wajant, H., Pfizenmaier, K. & Scheurich, P. Tumor necrosis factor signaling. Cell Death Differ 10:45–65 (2003).
Baud, V. & Karin, M. Signal transduction by tumor necrosis factor and its relatives. Trends Cell Biol 11:372–377 (2001).
Wertz, I. E. TNFR1-activated NF-kappaB signal transduction: regulation by the ubiquitin/proteasome system. Curr Opin Chem Biol 23:71–77 (2014).
Koo, H. J. et al. The analgesic and anti-inflammatory effects o Litsea japonica fruit are mediated via suppression of NF-kappaB and JNK/p38 MAPK activation. Int Immunopharmacol 22:84–97 (2014).
Jeong, Y. J. et al. Anti-Osteoarthriti Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate. PLoS One 10:e0134856 (2015).
Kim, J. et al. Extract o Litsea japonica ameliorates blood-retinal barrier breakdown in db/db mice. Endocrine 46:462–469 (2014).
Kim, J. et al. Litsea japonica extract inhibits neuronal apoptosis and the accumulation of advanced glycation end products in the diabetic mouse retina. Mol Med Rep 12:1075–1081 (2015).
Kim, J. et al. Litsea japonic Extract Inhibits Aldose Reductase Activity and Hyperglycemia-Induced Lenticular Sorbitol Accumulation in db/db Mice. Evid Based Complement Alternat Med 2015:747830 (2015).
Sohn, E. et al. Th Extract of Litsea japonica Reduced the Development of Diabetic Nephropathy via the Inhibition of Advanced Glycation End Products Accumulation in db/db Mice. Evid Based Complement Alternat Med 2013:769416 (2013).
Ham, Y. M. et al. Anti-inflammatory effect of litsenolide B2 isolated fro Litsea japonica fruit via suppressing NF-kappa B and MAPK pathways in LPS-induced RAW264.7 cells. J Funct Foods 13:80–88 (2015).
Paul, A. T., Gohil, V. M. & Bhutani, K. K. Modulating TNF-alpha signaling with natural products. Drug Discov Today 11:725–732 (2006).
Kim, J. E., Jun, S., Song, M., Kim, J. H. & Song, Y. J. The extract o Chrysanthemum indicum Linne inhibits EBV LMP1-induced NF-kappaB activation and the viability of EBV-transformed lymphoblastoid cell lines. Food Chem Toxicol 50:1524–1528 (2012).
Lee, S. Y. et al. Flavonoids from the leaves o Litsea japonica and their anti-complement activity. Phytother Res 19:273–276 (2005).
Min, B. S. et al. Lactones from the leaves o Litsea japonica and their anti-complement activity. J Nat Prod 66:1388–1390 (2003).
Kang, S. C., Lim, S. Y. & Song, Y. J. Lupeol is one of active components in the extract o Chrysanthemum indicum Linne that inhibits LMP1-induced NF-kappaB activation. PLoS One 8:e82688 (2013).
Song, Y. J. & Stinski, M. F. Inhibition of cell division by the human cytomegalovirus IE86 protein: role of the p53 pathway or cyclin-dependent kinase 1/cyclin B1. J Virol 79:2597–2603 (2005).
Lim, S. Y. & Song, Y. J. The extract o Tamarindus indica L. suppresses IKK beta activity and NF-kappa B-dependent lymphoblastoid cell line survival. Mol Cell Toxicol 9:243–248 (2013).
Kim, J. E., Kim, S. Y., Lim, S. Y., Kieff, E. & Song, Y. J. Role of Ca2+/calmodulin-dependent kinase II-IRAK1 interaction in LMP1-induced NF-kappaB activation. Mol Cell Biol 34:325–334 (2014).
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Won, J., Kim, JE., Choi, D.H. et al. Effects of compounds isolated from a Litsea japonica fruit extract on the TNF-α signaling pathway and cell viability. Mol. Cell. Toxicol. 12, 37–44 (2016). https://doi.org/10.1007/s13273-016-0006-1
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DOI: https://doi.org/10.1007/s13273-016-0006-1