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Antioxidant and anti-inflammatory activities of 3,5-dicaffeoylquinic acid isolated from Ligularia fischeri leaves

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Abstract

A potent anti-inflammatory compound was isolated and identified from leaves of Ligularia fischeri. The ethyl acetate fraction was used for purification of the compound and yielded 3,5-dicaffeoylquinic acid (DCQA). The identity of the compound was confirmed using 1H, 13C NMR, and MS analysis. DCQA showed strong DPPH (IC50=4.26 μg/mL), ABTS (TEAC value=0.9974) radical scavenging, and FRAP activities (=3.84 mmole of Trolox equivalent/g). DCQA effectively inhibited production of NO in RAW 264.7 cells induced using lipopolysaccharide (LPS) in a concentration dependent manner (50–250 μg/mL). DCQA significantly suppressed (p<0.05) up-regulation of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and tumor necrosis factor-α (TNF-α) gene expressions. The isolated DCQA can be useful for development of effective anti-inflammatory agents.

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Authors and Affiliations

  1. Department of Animal Products and Food Science, Kangwon National University, Chuncheon, Gangwon, 200-701, Korea

    Sunghyun Hong, Taewoo Joo & Jin-Woo Jhoo

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  1. Sunghyun Hong
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  2. Taewoo Joo
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Correspondence to Jin-Woo Jhoo.

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Hong, S., Joo, T. & Jhoo, JW. Antioxidant and anti-inflammatory activities of 3,5-dicaffeoylquinic acid isolated from Ligularia fischeri leaves. Food Sci Biotechnol 24, 257–263 (2015). https://doi.org/10.1007/s10068-015-0034-y

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  • DOI: https://doi.org/10.1007/s10068-015-0034-y

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