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Epigenome editing strategies for plants: a mini review

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Abstract

Recent development of genome editing (GE) tools has revolutionized crop improvement due to their ability to perform targeted genetic modifications. Among different generations of GE tools, CRISPR-Cas9 and its variants have become the most preferred tools due to their simplicity compared to the other GE tools. Recent studies have demonstrated employment of modified GE tools to introduce heritable epigenetic changes. In this review, we briefly describe what constitutes epigenome, epigenome editing (EGE), and some of the recently developed protein scaffold platforms such as, SunTag and SSSavi systems, being used for efficient EGE. We then discuss various CRISPR-Cas-based EGE approaches for modulating gene expression and the corresponding phenotype. Mainly, these approaches include targeted DNA methylation/demethylation, histone modification, and base editor-mediated conversion of promoter cytosines to other bases. Of these, the last EGE strategy involves genetic alteration to introduce epigenetic changes.

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Acknowledgements

GM is thankful to Indian Council of Agricultural Research (ICAR)-National Agricultural Science Fund (NASF), Govt. of India for the research grant (F. No. NASF/BGAM-9013/2022-23) and Department of Biotechnology, Govt. of India, for the research grant (BT/PR38410/GET/119/310/2020).

Funding

BA, PR, PA are funded by ICAR-NASF as SRF and ATS is currently funded by BITS-Pilani Hyderabad campus as an Institute Fellow.

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  1. Abirami T. Subramanian, Priyanka Roy and Balamurugan Aravind have contributed equally to this work.

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  1. Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, Hyderabad Campus, Hyderabad, Telangana, 500078, India

    Abirami T. Subramanian, Priyanka Roy, Balamurugan Aravind & Gireesha Mohannath

  2. ICAR-National Rice Research Institute, Cuttack, Odisha, 753006, India

    Akash P. Kumar

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Subramanian, A.T., Roy, P., Aravind, B. et al. Epigenome editing strategies for plants: a mini review. Nucleus (2024). https://doi.org/10.1007/s13237-024-00483-5

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