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Rapid multiplication of mature Eucalyptus hybrids through macro-and-micropropagation

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Abstract

Eucalyptus species are globally recognized and extensively planted hardwood trees whose flexibility, growth and diversity make them popular renewable sources of fibre and energy. Across the globe, the interest to create ways to develop significant hardwoods, like eucalypts, is rapidly growing. Although micropropagation offers a means to clonally propagate the desirable cultivars, the technique is often variety-specific for eucalypts. The present study aimed to expand our understanding of root initiation through macro-and-micropropagation in difficult to propagate superior full-sib matured hybrid (7-years-old trees) of eucalypts. Analysis of Variance (ANOVA) revealed that hormonal treatment of IBA (2,000–4,000 ppm) supplemented with Enterobacter sp. (106 cfu ml-1) significantly increased (p < 0.05) hybrid rooting through macropropagation. Notably, root initiation with maximum root development of 10.00% ± 0.91 and 3.75% ± 0.48 was observed at 3,000 ppm of IBA for hybrid genotypes FRI-PH3 (Corymbia torelliana × C. citriodora; 2A) and FRI-PH4 (E. pellita × E. urophylla; 1D), respectively. In the case of micropropagation studies, genotype FRI-4s (coppice shoots of E. tereticornis × E. camaldulensis, 10O) showed significant differences (p < 0.01) with various hormonal combinations (BAP, Kn and TDZ); and maximum number of shoot (4.0 ± 0.82) proliferation with average shoot length of 2.5 ± 0.15 cm was recorded in 0.5 mg L-1 BAP, ½ MS medium and 0.5 mg L-1 Kn in accordance to Duncan Multiple Range Test (DMRT). Afterward, for in vitro rooting, ¼ MS medium fortified with 1.0 mg L-1 IBA was proved to be the optimal medium for root induction, the maximum number of roots per explant (5.0 ± 0.91) with an average root length of 3.10 ± 0.15 cm was observed. Overall, the study signifies successful macro-and-micropropagation of mature eucalyptus hybrids through branch cuttings and nodal segments.

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Acknowledgements

The financial support by the Indian Council of Forestry Research & Education (ICFRE), Dehradun, under Grant No. 5-1/2017-RCS; dated 23rd June, 2017 is gratefully acknowledged. The authors are thankful to the Director, FRI for providing the research facilities and Ms. Upasana Chaubey for conducting experimentation on micropropagation. Authors wish to thank the Handling Editor and the anonymous reviewer for their constructive and positive comments in the manuscript.

Funding

The financial support by the Indian Council of Forestry Research & Education (ICFRE), Dehradun, under Grant No. 5-1/2017-RCS; dated 23rd June, 2017 is gratefully acknowledged. The authors are thankful to the Director, FRI for providing the research facilities.

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Authors and Affiliations

  1. Division of Genetics & Tree Improvement, Forest Research Institute, 248 195, Dehradun, Uttarakhand, India

    Maneesh S. Bhandari, Sandeep Maikhuri, Ajay Thakur & Arzoo Shamoon

  2. Tissue culture Discipline, Northern Regional Centre, Botanical Survey of India, 248 195, Dehradun, Uttarakhand, India

    Giriraj S. Panwar

  3. Forest Pathology Discipline, Division of Forest Protection, Forest Research Institute, 248 006, Dehradun, Uttarakhand, India

    Shailesh Pandey

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Contributions

MSB conceived the research ideas, conducted the analysis, data interpretation, wrote the first draft of the manuscript and led the writing. SM and AS performed the macropropagation-based field experimentation. GSP and AT performed the micropropagation experimentation. SP performed the experimentation on microbes and provided the bacterial culture isolates. All authors edited the draft and critically revised the manuscript.

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Correspondence to Maneesh S. Bhandari.

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Bhandari, M.S., Maikhuri, S., Thakur, A. et al. Rapid multiplication of mature Eucalyptus hybrids through macro-and-micropropagation. Nucleus 65, 379–389 (2022). https://doi.org/10.1007/s13237-022-00394-3

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