Skip to main content

Advertisement

SpringerLink
Log in

Enterobacter hormaechei as Plant Growth-Promoting Bacteria for Improvement in Lycopersicum esculentum

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Humans have been using natural resources for their daily life support. The population boom around the world has created a need to develop a new technique for increased crop production. Chemical fertilizers used in modern agriculture lead to pollution, besides increase in farming costs. To solve this problem, the present study deals with a natural halotolerant Enterobacter hormaechei which can fix N and solubilize desired macronutrients P and K. Enterobacter hormaechei was able to solubilize K-feldspar into potassium (97.5 ± 1.76 mg/L), tri-calcium phosphate into phosphate (99.7 ± 02 µg/mL) and it also produced IAA (47.87 ± 0.85 mg/L). Experiments, including morphological and chemical analysis, have provided a new growth pattern in Lycopersicum esculentum. Tomato seeds (Lycopersicum esculentum) treated with Enterobacter hormaechei enhanced biomass and an increase in shoot length when compared to control. It enhanced not only plant growth but modified the root architecture leading to improved crop productivity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Mrkovački N, Đalović I, Jošić D, Bjelić D, Brdar-Jokanović M (2016) The effect of PGPR strains on microbial abundance in maize rhizosphere in field conditions. Ratarstvo i Povrtarstvo 53(1):15–19

    Article  Google Scholar 

  2. Rai A, Nabti E (2017) Plant growth-promoting bacteria: importance in vegetable production. In: Microbial strategies for vegetable production. Springer, Cham, pp 23–48

  3. Majeed A, Abbasi MK, Hameed S, Imran A, Rahim N (2015) Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion. Front Microbiol 6:198

    Article  Google Scholar 

  4. Prasad R, Kumar M, Varma A (2015) Role of PGPR in soil fertility and plant health. In: Plant-growth-promoting rhizobacteria (PGPR) and medicinal plants. Springer, Cham, pp 247–260

  5. Verbon EH, Liberman LM (2016) Beneficial microbes affect endogenous mechanisms controlling root development. Trends Plant Sci 21(3):218–229

    Article  CAS  Google Scholar 

  6. Ilangumaran G, Smith DL (2017) Plant growth promoting rhizobacteria in amelioration of salinity stress: a systems biology perspective. Front Plant Sci 8:1768

    Article  Google Scholar 

  7. Timmusk S, Kim SB, Nevo E, Abd El Daim I, Ek B, Bergquist J (2015) Sfp-type PPTase inactivation promotes bacterial biofilm formation and ability to enhance wheat drought tolerance. Front Microbiol 6:387

    Article  Google Scholar 

  8. Zahid M, Abbasi MK, Hameed S, Rahim N (2015) Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.). Front Microbiol 6:207

  9. Pandey A, Trivedi P, Kumar B, Palni LMS (2006) Characterization of a phosphate solubilizing and antagonistic strain of Pseudomonas putida (B0) isolated from a sub-alpine location in the Indian central Himalaya. Curr Microbiol 53:102–107

    Article  CAS  Google Scholar 

  10. Rau N, Mishra V, Sharma M, Das MK, Ahaluwalia K, Sharma RS (2009) Evaluation of functional diversity in rhizobacterial taxa of a wild grass (Saccharumravennae) colonizing abandoned fly ash dumps in Delhi urban ecosystem. Soil Biol Biochem 41:813–821

    Article  CAS  Google Scholar 

  11. Ma Y, Prasad MNV, Rajkumar M, Freitas H (2011) Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils. Biotechnol Adv 29:248–258

    Article  CAS  Google Scholar 

  12. Chauhan H, Bagyaraj DJ, Selvakumar G, Sundaram SP (2015) Novel plant growth promoting rhizobacteria- prospects and potential. Appl Soil Ecol 95:38–53

    Article  Google Scholar 

  13. Raiola A, Rigano MM, Calafiore R, Frusciante L (2014) Enhancing the human-promoting effects of tomato fruit for biofortified food. Hindawi Publishing Corporation Mediators Inflamm

  14. INDIASTAT.COM (2020) Ministry of Agriculture and Farmers Welfare Govt of India

  15. Rajawat MVS, Singh S, TyagiSaxena SPAK (2016) A modified plate assay for rapid screening of potassium-solubilizing bacteria. Pedosphere 26(5):768–773

    Article  CAS  Google Scholar 

  16. Pikovskaya RI (1948) Mobilization of phosphates in soil in connection with the vital activities of some microbial species. Mikrobiol 17:362–370

    CAS  Google Scholar 

  17. Wilson PW, Knight SG (1947) Experiments in bacterial physiology. Burgess, Minneapolis, MN

    Google Scholar 

  18. Ehmann A (1977) The Van Urk-Salkowski reagent—a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives. J Chromatogr A 132:267–276

    Article  CAS  Google Scholar 

  19. Marques AP, Pires C, Moreira H, Rangel AO, Castro PM (2010) Assessment of the plant growth promotion abilities of six bacterial isolates using Zea mays as indicator plant. Soil Biol Biochem 42:1229–1235

    Article  CAS  Google Scholar 

  20. Radu S, Ahmad N, Ling FH, Reezal A (2003) Prevalence and resistance to antibiotics for Aeromonas species from retail fish in Malaysia. Int J Food Microbiol 81(3):261–266

    Article  CAS  Google Scholar 

  21. Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual: Cold Spring Harbor. Cold Spring Harbor Laboratory Press, New York, NY

  22. Lane D (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175

  23. Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Circular Calif Agric Expt Stat 347 (2nd edn)

  24. Steel RGD, Torrie JH (1980) Principles and procedures of statistics, 2nd edn. McGraw Hill Book Co Inc, New York, NY

    Google Scholar 

  25. Rogers A, McDonald K, Muehlbauer MF, Hoffman A, Koenig K, Newman L, Van der Lelie D (2012) Inoculation of hybrid poplar with the endophytic bacterium Enterobacter sp. 638 increases biomass but does not impact leaf level physiology. Gcb Bioenergy 4:364–370

    Article  Google Scholar 

  26. Laili NS, Radziah O, Zaharah SS (2017) Isolation and characterization of plant growth-promoting rhizobacteria (PGPR) and their effects on growth of strawberry (Fragaria Ananassa Duch.). Bangladesh J Bot 46:277–282

    Google Scholar 

  27. Singh RP, Jha P, Jha PN (2015) The plant-growth-promoting bacterium Klebsiella sp. SBP-8 confers induced systemic tolerance in wheat (Triticum aestivum) under salt stress. J Plant Physiol 184:57–67

    Article  CAS  Google Scholar 

  28. Singh RP, Nalwaya S, Jha PN (2017) The draft genome sequence of the plant growth promoting rhizospheric bacterium Enterobacter cloacae SBP-8. Genomics Data 12:81–83

    Article  Google Scholar 

  29. Coulson TJ, Patten CL (2015) Complete genome sequence of Enterobacter cloacae UW5, a rhizobacterium capable of high levels of indole-3-acetic acid production. Genome Announc. 3(4).

  30. Orhan F (2016) Alleviation of salt stress by halotolerant and halophilic plant growth-promoting bacteria in wheat (Triticum aestivum). Braz J Microbial 47:621–627

    Article  CAS  Google Scholar 

  31. Gupta M, Kiran S, Gulati A, Singh B, Tewari R (2012) Isolation and identification of phosphate solubilizing bacteria able to enhance the growth and aloin-A biosynthesis of Aloe barbadensis Miller. Microbiol Res 167:358–363

    Article  CAS  Google Scholar 

  32. Delgado M, Mendez J, Rodríguez-Herrera R, Aguilar CN, Cruz-Hernández M, Balagurusamy N (2014) Characterization of phosphate-solubilizing bacteria isolated from the arid soils of a semi-desert region of north-east Mexico. Biol Agric Hortic 30:211–217

    Article  Google Scholar 

  33. Singh RP, Nalwaya S, Jha PN (2017) The draft genome sequence of the plant growth promoting rhizospheric bacterium Enterobacter cloacae SBP-8. Genom data 12:81–83

    Article  Google Scholar 

  34. Andrés-Barrao C, Lafi FF, Alam I, De Zélicourt A, Eida AA, Bokhari A, Alzubaidy H, Bajic VB, Hirt H, Saad MM (2017) Complete genome sequence analysis of Enterobacter sp. SA187, a plant multi-stress tolerance promoting endophytic bacterium. Front Microbiol 8:2023.

  35. Bhattacharyya PN, Jha DK (2012) Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World J Microbiol Biotechnol 28:1327–1350

    Article  CAS  Google Scholar 

  36. Goldstein AH, Liu ST (1987) Molecular cloning and regulation of amineral phosphate solubilizing gene from Erwinia herbicola. Nat Biotechnol 5:72–74

    Article  CAS  Google Scholar 

  37. Sheng XF (2005) Growth promotion and increased potassium uptake of cotton and rape by a potassium releasing strain of Bacillus edaphicus. Soil Biol Biochem 37:1918–1922

    Article  CAS  Google Scholar 

  38. Ahmad A, Ghulam J, Mohammad SA, SyedMohammad MSNR (2009) Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. J Agric Biol Sci 1:48–58

    Google Scholar 

  39. Niu B, Paulson JN, Zheng X, Kolter R (2017) Simplified and representative bacterial community of maize roots. Proc Natl Acad Sci 114:E2450–E2459

    Article  CAS  Google Scholar 

  40. Egamberdieva D, Botir H, Hashem A, Abd-Allah EF (2014) Characterization of salt tolerant Enterobacter hormaechei strain associated with tomato root grown in arid saline soil. J Pure Appl Microbiol 8:4231–4239

    CAS  Google Scholar 

  41. Dimkpa C, Weinand T, Asch F (2009) Plant–rhizobacteria interactions alleviate abiotic stress conditions. Plant Cell Environ 32:1682–1694

    Article  CAS  Google Scholar 

  42. Glick BR (2012) Plant growth-promoting bacteria: mechanisms and applications. Scientifica 963401–963401

  43. Zakria M, Ohsako A, Saeki Y, Yamamoto A, Akao S (2008) Colonization and growth promotion characteristics of Enterobacter sp. and Herbaspirillum sp. on Brassica oleracea. Soil Sci Plant Nutr 54:507–516

    Article  Google Scholar 

  44. Zhao Y (2010) Auxin biosynthesis and its role in plant development. Ann Rev Plant Biol 61:49–64

    Article  CAS  Google Scholar 

  45. Taghavi S, Van Der Lelie D, Hoffman A, Zhang YB, Walla MD, Vangronsveld J, Newman L, Monchy S (2010) Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638. PLoS Genet 6:e1000943.

  46. López-Bucio J, Cruz-Ramırez A, Herrera-Estrella L (2003) The role of nutrient availability in regulating root architecture. Curr Opin Plant Biol 6:280–287

    Article  Google Scholar 

  47. Guo DJ, Singh RK, Singh P, Li DP, Sharma A, Xing YX, Song XP, Yang LT, Li YR (2020) Complete genome sequence of Enterobacter roggenkampii ED5, a nitrogen fixing plant growth promoting endophytic bacterium with biocontrol and stress tolerance properties, isolated from sugarcane root. Front Microbiol 11:2270

    Google Scholar 

  48. Fitter AH (1987) An architectural approach to the comparative ecology of plant root systems. New Phytol 106:61–77

    Article  Google Scholar 

  49. Fitter AH, Stickland TR, Harvey ML, Wilson GW (1991) Architectural analysis of plant root systems 1. Architectural correlates of exploitation efficiency. New Phytol 118:375–382

    Article  Google Scholar 

  50. Dunbabin V, Diggle A, Rengel Z (2003) Is there an optimal root architecture for nitrate capture in leaching environments? Plant Cell Environ 26:835–844

    Article  Google Scholar 

  51. Morales A, Garland JL, Lim DV (1996) Survival of potentially pathogenic human-associated bacteria in the rhizosphere of hydroponically grown wheat. FEMS Microbiol Ecol 20:155–162

    Article  CAS  Google Scholar 

  52. Vacheron J, Desbrosses G, Bouffaud ML, Touraine B, Moënne-Loccoz Y, Muller D, Legendre L, Wisniewski-Dye F, Prigent-Combaret C (2013) Plant growth-promoting rhizobacteria and root system functioning. Front Plant Sci 4:356

    Article  Google Scholar 

Download references

Acknowledgements

CSIR-CSMCRI Communication No: 154/2017. The financial funding received from Council of Scientific and Industrial Research (CSIR), New Delhi, India (project K-TEN 0105) is thankfully acknowledged. The analytical department of CSIR-CSMCRI acknowledged for support and cooperation throughout the analysis of samples. Academy of Scientific & Innovative (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute is gratefully acknowledged.

Author information

Authors and Affiliations

  1. Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, 364002, India

    Bablesh Ranawat, Pooja Bachani, Aneesha Singh & Sandhya Mishra

  2. Applied Phycology and Biotechnology Division, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, 364002, India

    Bablesh Ranawat, Pooja Bachani, Aneesha Singh & Sandhya Mishra

Authors
  1. Bablesh Ranawat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pooja Bachani
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aneesha Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sandhya Mishra
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

AS: Idea and conceptualization of experiment, data interpretation and critical analysis of the data, manuscript correction. BR: Experiment design, Experiment execution, writing manuscript, data analysis and interpretation. PB: Helped in experiment execution. SM: Critical suggestion and correction in manuscript.

Corresponding author

Correspondence to Aneesha Singh.

Ethics declarations

Conflict of interest

The author(s) declare(s) that there is no conflict of interest regarding the publication of this article.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ranawat, B., Bachani, P., Singh, A. et al. Enterobacter hormaechei as Plant Growth-Promoting Bacteria for Improvement in Lycopersicum esculentum. Curr Microbiol 78, 1208–1217 (2021). https://doi.org/10.1007/s00284-021-02368-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-021-02368-1

Search

Navigation