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Inter-specific hybrid sesame with high lignan content in oil reveals increased expression of sesamin synthase gene

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Abstract

The presence of lignans (sesamin, sesamol) adds pharmaceutical value in the sesame oil. Wild sesame, Sesamum mulayanum, though less oil-yielding but contains remarkably higher lignan in oil. We performed an interspecific hybridization between cultivated Indian sesame (S. indicum) and S. mulayanum to bring a better oil profile in the cultivated sesame. The selected recombinant lines of F6 generation showed high oil content with a superior lignan profile and manifested distinct phenotypic selection traits. Sesamin synthase is an essential enzyme in the lignan biosynthetic pathway. We studied the gene behind this enzyme during seed development in the parents through semi-quantitative and qRT-PCR analysis. The recombinant lines with high sesamin content showed increased expression of sesamin synthase gene. This gene is a potential candidate for allele-based molecular marker development in future sesame breeding programme.

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Acknowledgements

We are indebted to the Director, Bose Institute for providing the infrastructural support. We are thankful to Dr. Mahua Ghosh, Department of Chemical Technology, The University of Calcutta for her valuable suggestions on the oil extraction procedure. The technical assistance of Mr. Jadab Ghosh, Mrs. Kaberi Ghosh, Mr. Mrinal Das, Mr. Swaroop Biswas and Mrs. Sheolee Ghosh Chakraborty is acknowledged. This work was financially supported by intramural grant from Bose Institute to GG, and UCG fellowship to DD.

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Authors and Affiliations

  1. Division of Plant Biology, Bose Institute (Main Campus), 93/1 APC Road, Kolkata, 700009, India

    Debabrata Dutta & Gaurab Gangopadhyay

  2. College of Agriculture, Tripura, India

    Ranjana Prasad

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  1. Debabrata Dutta
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  2. Ranjana Prasad
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  3. Gaurab Gangopadhyay
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Contributions

Debabrata Dutta: Formal analysis, investigation (HPLC, qRT-PCR, gel electrophoresis), validation, visualization, writing—original draft, writing—review and editing. Ranjana Prasad: Investigation (hybridization, SSR, seed phonemics). Gaurab Gangopadhyay: Conceptualization, funding acquisition, resources, supervision, writing—original draft, writing—review & editing.

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Correspondence to Gaurab Gangopadhyay.

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Dutta, D., Prasad, R. & Gangopadhyay, G. Inter-specific hybrid sesame with high lignan content in oil reveals increased expression of sesamin synthase gene. Nucleus 65, 7–18 (2022). https://doi.org/10.1007/s13237-021-00354-3

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