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Combination of 2-methoxyestradiol (2ME) and cyclophosphamide (CP) inhibits tumour progression in S-180 mouse tumour model system

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Abstract

2-Methoxyestradiol (2ME), an anti-neoplastic and anti-angiogenic agent, has been used in preclinical research for cancer treatment. Cyclophosphamide (CP), the anti-neoplastic and alkaloid drug, has long been used as a traditional chemotherapeutic drug against different human neoplastic diseases. Our earlier studies showed that 2ME inhibit angiogenesis and cell proliferation rate differently in vivo and in vitro tumour models. But little work has been done on the combination effect of 2ME and CP on tumour model system. Therefore, the present study has been oriented to evaluate the efficacy and safety of combination effect of 2ME and CP on Sarcoma-180 tumour model system. In the present paper, an attempt has also been taken to find out whether the combination effect of 2ME and CP is less toxic than the traditional chemotherapeutic drug-CP, considering S-180 tumour bearing mouse bone marrow and germ cell toxicity as parameters and chromosomal aberrations, metaphase index and sperm head abnormality as biological endpoints. The result of these studies showed significant regression in tumour growth with inhibition of viable cancer cell population and less toxicity in the somatic and germinal cell of tumour bearing mouse.

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Acknowledgement

Samarendra Nath Banerjee acknowledges UGC, New Delhi (Ref.F.42‐602/2013 (SR) dated 22.03.2013 MRP) for the financial support. The paper is dedicated to late Professor Samar Chakrabarti, Cancer Cytogenetic Unit, Department of Zoology, Burdwan University. Authors are grateful to Dr. R.N. Boral and Dr. C.K. Panda, CNCI for their valuable help in this work. Authors are also thankful to the Principal Dr. Saswati Sanyal of Rammohan College, Kolkata for her support and encouragement.

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Authors and Affiliations

  1. Department of Zoology, Rammohan College, 102/1, Raja Rammohan Sarani, Kolkata, 700009, India

    Srabantika Mallick, Sudipta Chowdhury, Anasua Banerjee & Samarendra Nath Banerjee

  2. Department of Physiology, University of Kalyani, Kalyani, Nadia, India

    Goutam Paul

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  1. Srabantika Mallick
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  2. Sudipta Chowdhury
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  3. Anasua Banerjee
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  4. Goutam Paul
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  5. Samarendra Nath Banerjee
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Correspondence to Srabantika Mallick.

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Mallick, S., Chowdhury, S., Banerjee, A. et al. Combination of 2-methoxyestradiol (2ME) and cyclophosphamide (CP) inhibits tumour progression in S-180 mouse tumour model system. Nucleus 60, 147–153 (2017). https://doi.org/10.1007/s13237-017-0204-9

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