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Maternal Serum Vitamin E Levels and its Association with Cord Blood Telomere Length and Mitochondrial DNA Copy Number in Preterm Premature Rupture of Membranes

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Abstract

Background and Objective

Oxidative stress is one of the pathophysiological factors of pPROM and Vit. E being antioxidant may have preventive role. Study was conducted to estimate maternal serum vitamin E levels and cord blood oxidative stress markers in pPROM cases.

Methods

This was a case–control study including 40 pPROM cases and 40 controls. Maternal serum vitamin E levels were measured at recruitment. Cord blood was collected at delivery for estimation of telomere length and mtDNA copy number as oxidative stress markers. Levels were compared using student’s t test or Mann Whitney test. For correlation Pearson coefficient was used.

Results

Maternal serum vitamin E levels were normal in pPROM cases. Cord blood telomere length was more in pPROM than controls (428.99 ± 290.65 vs 322.35 ± 180.33) (p value 0.05). Cord blood mtDNA copy number was more in pPROM than controls (516.46 ± 443.55 vs 384.77 ± 328.27) (p value 0.13) though it was not significant. mtDNA copy number had negative correlation with Vit. E levels but it was statistically not significant (p value 0.49). There was no association of vitamin E levels with telomere length (p value 0.95).

Interpretation and Conclusion

pPROM was not associated with vitamin E deficiency. There was insignificant oxidative stress in cord blood as measured by mtDNA copy number but cord blood telomere length measurement did not detect any oxidative stress in pPPROM cases.

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Funding

No funding was received for the study.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, 110095, Delhi, India

    Rupa Kumari, Amita Suneja, Kiran Guleria & Rashmi Malik

  2. Department of Biochemistry, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, 110095, Delhi, India

    Mohit Mehndiratta

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  1. Rupa Kumari
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  2. Amita Suneja
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  3. Mohit Mehndiratta
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  4. Kiran Guleria
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  5. Rashmi Malik
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Dr RK, Dr A S and Dr MM. The first draft of the manuscript was written by Dr RK, Dr RM and Dr AS. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Rashmi Malik.

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Conflict of interest

The authors declare that they have no potential conflict of interest.

Ethical Approval

The study was approved by the Institutional Ethical Committee—Human Research of University College of Medical Sciences and GTB Hospital, Delhi. The study was performed in accordance with the ethical standards as laid down in the 1964 Declaration of Helsinki and its later amendments.

Informed Consent

Written voluntary informed consent to participate in the study was obtained from all the participants.

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Rupa Kumari (MD), Senior Resident, Department of Obstetrics and Gynecology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi, India; Amita Suneja (MD), Director Professor and Head, Department of Obstetrics and Gynecology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi, India; Mohit Mehndiratta, Associate Professor, Department of Biochemistry, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi, India; Kiran Guleria (MD), Director Professor, Department of Obstetrics and Gynecology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi, India; Rashmi Malik, Associate Professor, Department of Obstetrics and Gynecology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi, India.

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Kumari, R., Suneja, A., Mehndiratta, M. et al. Maternal Serum Vitamin E Levels and its Association with Cord Blood Telomere Length and Mitochondrial DNA Copy Number in Preterm Premature Rupture of Membranes. J Obstet Gynecol India 73, 9–14 (2023). https://doi.org/10.1007/s13224-022-01684-1

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  • DOI: https://doi.org/10.1007/s13224-022-01684-1

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