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Production and partial characterization of the exopolysaccharide from Pleurotus sajor caju

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Abstract

Purpose

Microbial exopolysaccharides (EPSs) are very important because they are used in biotechnological applications in different industrial areas. The aim of the study was to determine the best EPS producer Pleurotus sp., to optimize EPS production and to perform partial purification and characterization of the produced EPS.

Methods

After the production conditions were optimized, the EPS was isolated and partially purified. EPS was characterized by HP-TLC, 1H-NMR, FT-IR, and TGA. Hydroxyl, superoxide, and DPPH radical scavenging activities of the EPS were also investigated spectrophotometrically.

Result

The best EPS producer and its incubation period in submerged fermentation were determined as Pleurotus sajor caju and on 5 days, respectively. Culture conditions to increase EPS production were optimized as follows (in per liter): 90 g of glucose, 10 g of yeast extract, 10 g of peptone, and 100 mM of Mg2+. The optimal initial pH, temperature, and an agitation rate of culture were determined as 5.0, 25 °C, and 150 rate min−1, respectively. The highest EPS production was determined as 33.32 ± 1.6 g L−1. After isolation of EPS, one active fraction was obtained by gel filtration chromatography. EPS is composed mainly of glucose according to HP-TLC analysis.

Conclusion

To the results, EPS had a complex structure by having carbohydrate and protein contents. The produced EPS had high degradation temperature as well as high antioxidant activity.

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Acknowledgments

This study was supported by the Scientific Research Project of Dokuz Eylül University (project number = 2014.KB.FEN.037).

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Authors and Affiliations

  1. Faculty of Science, Chemistry Department, Biochemistry Division, Dokuz Eylül University, 35160, Buca, Izmir, Turkey

    Raziye Ozturk Urek

  2. Graduate School of Natural and Applied Sciences, Biotechnology Department, Dokuz Eylül University, 35160, Buca, Izmir, Turkey

    Seda Ilgin

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  1. Raziye Ozturk Urek
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Correspondence to Raziye Ozturk Urek.

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Ozturk Urek, R., Ilgin, S. Production and partial characterization of the exopolysaccharide from Pleurotus sajor caju. Ann Microbiol 69, 1201–1210 (2019). https://doi.org/10.1007/s13213-019-01502-6

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