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Optimization of fermentation conditions for enhancing extracellular production of L-asparaginase, an anti-leukemic agent, by newly isolated Streptomyces brollosae NEAE-115 using solid state fermentation

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Abstract

Optimization of the fermentation conditions for extracellular production of L-asparaginase by Streptomyces brollosae NEAE-115 under solid state fermentation was investigated. The Plackett–Burman experimental design was used to screen 16 independent variables (incubation time, moisture content, inoculum size, temperature, pH, soybean meal + wheat bran, dextrose, fructose, L-asparagine, yeast extract, KNO3, K2HPO4, MgSO4.7H2O, NaCl, FeSO4. 7H2O, CaCl2) and three dummy variables. The most significant independent variables found to affect enzyme production, namely soybean + wheat bran (X6), L-asparagine (X9) and K2HPO4 (X12), were further optimized by the central composite design. We found that L-asparaginase production by S. brollosae NEAE-115 was 47.66, 129.92 and 145.57 units per gram dry substrate (U/gds) after an initial survey using “soybean meal + wheat bran” as a substrate for L-asparaginase production (step 1), statistical optimization by Plackett–Burman design (step 2) and further optimization by the central composite design (step 3), respectively, with a fold of increase of 3.05.

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Acknowledgments

The author gratefully acknowledges the Science and Technology Development Fund (STDF), Egypt, for their financial support of this paper which is a part of the Grant No. 4943.

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Authors and Affiliations

  1. Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, New Borg El- Arab City, 21934, Alexandria, Egypt

    Noura El-Ahmady El-Naggar

  2. Department of Agricultural Microbiology at National Research Center, Cairo, Egypt

    Hassan Moawad

  3. Chemistry of Natural and Microbial Products Department, Pharmaceutical Industry Division, National Research Centre, 33 El Buhouth St. (Former El Tahrir St.), 12622, Dokki, Giza, Egypt

    Nayera A. M. Abdelwahed

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  1. Noura El-Ahmady El-Naggar
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  2. Hassan Moawad
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  3. Nayera A. M. Abdelwahed
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Correspondence to Noura El-Ahmady El-Naggar.

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El-Ahmady El-Naggar, N., Moawad, H. & Abdelwahed, N.A.M. Optimization of fermentation conditions for enhancing extracellular production of L-asparaginase, an anti-leukemic agent, by newly isolated Streptomyces brollosae NEAE-115 using solid state fermentation. Ann Microbiol 67, 1–15 (2017). https://doi.org/10.1007/s13213-016-1231-5

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