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Distribution of arbuscular mycorrhizal fungi in four semi-mangrove plant communities

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Abstract

To better understand the diversity and species composition of arbuscular mycorrhizal fungi (AMF) in mangrove ecosystems, the AMF colonization and distribution in four semi-mangrove plant communities were investigated. Typical AMF hyphal, vesicle and arbuscular structures were commonly observed in all the root samples, indicating that AMF are important components on the landward fringe of mangrove habitats. AMF spores were extracted from the rhizospheric soils, and an SSU rDNA fragment from each spore morph-type was amplified and sequenced for species identification. AMF species composition and diversity in the roots of each semi-mangrove species were also analyzed based on an SSU-ITS-LSU fragment, which was amplified, cloned and sequenced from root samples. In total, 11 unique AMF sequences were obtained from spores and 172 from roots. Phylogenetic analyses indicated that the sequences from the soil and roots were grouped into 5 and 14 phylotypes, respectively. AMF from six genera including Acaulospora, Claroideoglomus, Diversispora, Funneliformis, Paraglomus, and Rhizophagus were identified, with a further six phylotypes from the Glomeraceae family that could not be identified to the genus level. The AMF genus composition in the investigated semi-mangrove communities was very similar to that in the intertidal zone of this mangrove ecosystem and other investigated mangrove ecosystems, implying possible fungal adaptation to mangrove conditions.

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Acknowledgments

This research was supported financially by the National Natural Science Foundation of China (31371567), the Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (2012LYM_0049), the Science Foundation on Cultivating Young Teachers from South China Normal University and the Research Fund Program of Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology. The study sponsor had no role in the study design, data collection, data analysis, data interpretation, or the writing of the report.

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Authors and Affiliations

  1. Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, Guangzhou, 510631, China

    Yutao Wang, Ting Li, Yingwei Li & Shaoshan Li

  2. Guangzhou Water Quality Monitoring Center, Guangzhou, 510010, China

    Qiu Qiu

  3. Guangdong Key Laboratory of Plant Resources, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China

    Yutao Wang & Guorong Xin

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  1. Yutao Wang
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  2. Ting Li
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  3. Yingwei Li
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  4. Qiu Qiu
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  5. Shaoshan Li
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  6. Guorong Xin
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Correspondence to Guorong Xin.

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Wang, Y., Li, T., Li, Y. et al. Distribution of arbuscular mycorrhizal fungi in four semi-mangrove plant communities. Ann Microbiol 65, 603–610 (2015). https://doi.org/10.1007/s13213-014-0896-x

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  • DOI: https://doi.org/10.1007/s13213-014-0896-x

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