Abstract
The present study provides an integrated method for utilizing the wastewaters from second generation (2G) ethanol pretreatment plant for microalgal biomass and lipid production. The study was conducted using a mixture of wastewaters (referred as MW; pH 4.3) generated after washing of acidic and alkaline-soaked lignocellulosic biomass prior to pretreatment process. The growth studies indicated that the thermotolerant strain of Chlorella pyrenoidosa (C. pyrenoidosa) M18 exhibited higher cell proliferation in wastewater as compared to freshwater. About 20–25% enhancement in biomass (509 mg L−1 d−1 ± 3.09) and lipid productivity (146 mg L−1 d−1 ± 1.34) was observed in MW. The total chlorophyll content and variable fluorescence by maximum fluorescence (Fv/Fm) ratio of strain cultivated in MW were 10.32 µg mL−1 and 0.75, respectively. The use of MW also enhanced the content of saturated and monounsaturated fatty acids in total lipid. The exhausted wastewater medium obtained after harvesting the auto-flocculated biomass was also reused up to three successive growth cycles. The recycled medium without any nutrient addition could be used for two subsequent rounds with enhanced biomass (520 mg L−1 d−1 ± 4.07) and lipid (157.71 mg L−1 d−1 ± 1.09) productivities. This synergistic approach of cultivating thermotolerant microalgae with wastewater from 2G pretreatment plant provides an economical setup for development of commercial algal biofuel technology.
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References
Amenorfenyo DK, Huang X, Zhang Y, Zeng Q, Zhang N, Ren J, Huang Q (2019) Microalgae brewery wastewater treatment: potentials, benefits and the challenges. Int J Environ Res Public Health 16:1910. https://doi.org/10.3390/ijerph16111910
Anne NN, Kuehnle VA, Schurr AR, Robert J (2018) Producing and altering microbial fermentation products using non-commonly used lignocellulosic hydrolysates US 2018/0066288 A1
Bligh E, Dyer W (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917. https://doi.org/10.1139/o59-099
Bošnjakovi M, Sinaga N (2020) The perspective of large-scale production of algae biodiesel. Appl Sci 10:8181. https://doi.org/10.3390/app10228181
Chen CY, Durbin EG (1994) Effects of pH on the growth and carbon uptake of marine phytoplankton. Mar Ecol Prog Ser 109:83–94. https://doi.org/10.3354/meps109083
Chen L, Liu T, Zhang W, Chen X, Wang J (2012) Biodiesel production from algae oil high in free fatty acids by two-step catalytic conversion. Bioresour Technol 111:208–214. https://doi.org/10.1016/j.biortech.2012.02.033
Chiranjeevi P, Mohan SV (2016) Critical parametric influence on microalgae cultivation towards maximizing biomass growth with simultaneous lipid productivity. Renew Ener 98:64–71. https://doi.org/10.1016/j.renene.2016.03.063
Chisti Y (2013) Constraints to commercialization of algal fuels. J Biotechnol 167:201–214. https://doi.org/10.1016/j.jbiotec.2013.07.020
Chiu SY, Kao CY, Chen TY, Chang YB, Kuo CM, Lin CS (2015) Cultivation of microalgal Chlorella for biomass and lipid production using wastewater as nutrient resource. Bioresour Technol 184:179–189. https://doi.org/10.1016/j.biortech.2014.11.080
Cortes AA, Fortun SS, Garcia M, Bartolome MC (2018) Effects of pH on the growth rate exhibited of the wild-type and Cd-resistant Dictyosphaerium chlorelloides strains. Limnetica 37:229–238. https://doi.org/10.23818/limn.37.19
Elias AG, Spijkerman E, Proschold T (2005) Effect of external pH on the growth, photosynthesis and photosynthetic electron transport of Chlamydomonas acidophila Negoro, isolated from an extremely acidic lake (pH 2.6). Plant Cell Environ 28:1218–1229. https://doi.org/10.1111/j.1365-3040.2005.01357.x
Fakhry EM, Maghraby DM (2015) Lipid accumulation in response to nitrogen limitation and variation of temperature in Nannochloropsis salina. Botan Stud 56:6. https://doi.org/10.1186/s40529-015-0085-7
Fan J, Cui Y, Wan M, Wang W, Li Y (2014) Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosa under three nutrition stressors. Biotechnol Biofuel 7:17. https://doi.org/10.1186/1754-6834-7-17
Ferronato N, Torretta V (2019) Waste mismanagement in developing countries: a review of global issues. Int J Environ Res Public Health 16(6):1060. https://doi.org/10.3390/ijerph16061060
Gaitán HAR, Humberto RF, Maldonado AIL, Jauregui JA, Contreras JAV, Breceda HF (2016) Biomass production and quality estimation of Chlorella Vulgaris (clv2) under large scale production conditions. J Exp Biol Agric Sci 4:493–498. https://doi.org/10.18006/2016.4(5).493.498
Gaur R, Semwal S, Raj T, Lamba BY, Ramu E, Gupta RP, Kumar R, Puri SK (2017) Intensification of steam explosion and structural intricacies impacting sugar recovery. Bioresour Technol 241:692–700. https://doi.org/10.1016/j.biortech.2017.05.208
Gross W (2000) Ecophysiology of algae living in highly acidic environments. Hydrobiogia 433:31–37
Hena S, Fatimah S, Tabassum S (2015) Cultivation of algae consortium in a dairy farm wastewater for biodiesel production. Water Resour Ind 10:1–14. https://doi.org/10.1016/j.wri.2015.02.002
Humbird DD, Tao L, Kinchin C, Hsu D, Aden A, Shoen P, Lukas B, Olthof M, Worley M, Sexton D, Dudgeon D (2011) Process design and economics for biochemical conversion of lignocellulosic biomass to ethanol: dilute-acid pretreatment and enzymatic hydrolysis of corn stover. NREL/TP-5100-47764. National Renewable Energy Laboratory, Golden
Juneja A, Ceballos RM, Murthy GS (2013) Effects of environmental factors and nutrient availability on the biochemical composition of algae for biofuels production: a review. Energies 6:4607–4638. https://doi.org/10.3390/en6094607
Kapoor M, Soam S, Agrawal R, Gupta RP, Tuli D, Kumar R (2016) Pilot scale dilute acid pretreatment of rice straw and fermentable sugar recovery at high solid loadings. Bioresour Technol 224:688–693. https://doi.org/10.1016/j.biortech.2016.11.032
Khan MY, Russell RL, Welch WA, Cocker DR, Ghosh S (2012) Impact of algae biofuel on in-use gaseous and particulate emissions from a marine vessel. Energ Fuel 26:6137–6143
Kumar KS, Prasanthkumar S, Ray JG (2017) Biomass yield, oil productivity and fatty acid profile of Chlorella lobophora cultivated in diverse eutrophic wastewaters. Biocatal Agric Biotechnol 11:338–344. https://doi.org/10.1016/j.bcab.2017.08.006
Kumar M, SunY RR, Pandey A, Thakur IS, Tsang D (2020) Algae as potential feedstock for the production of biofuels and value-added products: opportunities and challenges. Sci Total Environ 716:137116. https://doi.org/10.1016/j.scitotenv.2020.137116
Kuo CM, Chen YT, Lin TH, Kao CY, Lai JT, Chang JS, Lin CS (2015) Cultivation of Chlorella sp. GD using piggery wastewater for biomass and lipid production. Bioresour Technol 194:326–333. https://doi.org/10.1016/j.biortech.2015.07.026
Lam MK, Mohammad IY, Uemura Y, Lim WL, Khoo CG, Lee KT, Ong HC (2017) Cultivation of Chlorella vulgaris using nutrients source from domestic wastewater for biodiesel production: growth condition and kinetic studies. Renew Energy 103:197–207. https://doi.org/10.1016/j.renene.2016.11.032
Lee SY, Sankaran R, Chew KW, Tan CH, Krishnamoorthy R, Chu DT, Show PL (2019) Waste to bioenergy: a review on the recent conversion technologies. BMC Energy 1:4. https://doi.org/10.1186/s42500-019-0004-7
Lehesto HM, Perttu MK, Fredrik SJ (2014) Process and microorganisms for production of lipids from lignocellulosic wastes or residues. US patent 8,697,403 B2
Liu L, Pohnert G, Wei D (2016) Extracellular metabolites from industrial microalgae and their biotechnological potential. Mar Drug 14:191. https://doi.org/10.3390/md14100191
Loftus SE, Johnson ZI (2019) Reused cultivation water accumulates dissolved organic carbon and uniquely influences different marine microalgae. Front Bioeng Biotechnol 7:101. https://doi.org/10.3389/fbioe.2019.00101
Ma C, Wen H, Xing D, Pei X, Zhu N, Ren B (2017) Molasses wastewater treatment and lipid production at low temperature conditions by a microalgal mutant Scenedesmus sp. Z-4. Biotechnol Biofuel 10:111. https://doi.org/10.1186/s13068-017-0797-x
Mehta P, Rani R, Gupta RP, Mathur AS, Puri SK (2018) Biomass and lipid production of a novel freshwater thermo-tolerant mutant strain of Chlorella pyrenoidosa NCIM 2738 in seawater salinity recycled medium. Algal Res 36:88–95. https://doi.org/10.1016/j.algal.2018.10.015
Mehta P, Jackson BA, Nwoba EG, Vadiveloo A, Bahri PA, Mathur AS, Moheimani NR (2019) Continuous non-destructive hydrocarbon extraction from Botryococcus braunii BOT-22. Algal Res 41:101537. https://doi.org/10.1016/j.algal.2019.101537
Miazek K, Remacle C, Richel A, Goffin D (2014) Effect of lignocellulose related compounds on microalgae growth and product biosynthesis: a review. Energies 7:4446–4481. https://doi.org/10.3390/en7074446
Mohsenpour SF, Hennige S, Willoughby N, Adeloye A, Gutierrez T (2021) Integrating micro-algae into wastewater treatment: A review. Sci Total Environ 752:142168. https://doi.org/10.1016/j.scitotenv.2020.142168
Nam K, Lee H, Heo SW, Chang YK (2017) Cultivation of Chlorella vulgaris with swine wastewater and potential for algal biodiesel production. J Appl Phycol 29:1171–1178. https://doi.org/10.1007/s10811-016-0987-0
Pruvost J, Vooren GV, Gouic BL, Mossion AC, Legrand J (2011) Systematic investigation of biomass and lipid productivity by microalgae in photobioreactors for biodiesel application. Bioresour Technol 102:150–158. https://doi.org/10.1016/j.biortech.2010.06.153
Qiu R, Gao S, Lopez PA, Ogden KL (2017) Effects of pH on cell growth, lipid production and CO2 addition of microalgae Chlorella sorokiniana. Algal Res 28:192–199. https://doi.org/10.1016/j.algal.2017.11.004
Razzak SA, Hossain MM, Lucky RA, Bassi AS, de Lasa H (2013) Integrated CO2 capture, wastewater treatment and biofuel production by microalgae culturing- a review. Renew Sustain Energy Rev 27:622–653. https://doi.org/10.1016/j.rser.2013.05.063
Ritchie RJ (2006) Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents. Photosynth Res 89:27–41. https://doi.org/10.1007/s11120-006-9065-9
Singh D, Mehta P, Saxena R, Barrow CJ, Puri M, Tuli DK, Mathur AS (2018) Development of continuous cultivation process for oil production through bioconversion of minimally treated waste streams from second-generation bioethanol production. J Chem Technol Biotechnol 93:3018–3027. https://doi.org/10.1002/jctb.5660
Sluiter AD, Hyman C, Payne Wolfe J (2008) Determination of insoluble solids in pretreated biomass material. NREL/TP-510-42627. National Renewable Energy Laboratory, Golden
Tan XB, Zhao XC, Yang LB, Liao JY, Zhou YY (2018) Enhanced biomass and lipid production for cultivating Chlorella pyrenoidosa in anaerobically digested starch wastewater using various carbon sources and up-scaling culture outdoors. Biochem Eng J 135:105–114. https://doi.org/10.1016/j.bej.2018.04.005
Valta K, Kosanovic T, Malamis D, Moustakas K, Loizidou M (2015) Overview of water usage and wastewater management in the food and beverage industry. Desalin Water Treat 53:3335–3347. https://doi.org/10.1080/19443994.2014.934100
White S, Anandraj A, Bux F (2011) PAM fluorometry as a tool to assess microalgal nutrient stress and monitor cellular neutral lipids. Bioresour Technol 102:1675–1682. https://doi.org/10.1016/j.biortech.2010.09.097
Wouter KF, Johan RH, Petrus WNJ, Winde De, Hendrik J (2011) Novel microorganism and its use in lignocellulose detoxification. US Patent 2011(0086395):A1
Yang J, Xu M, Zhang XZ, Hu QA, Sommerfeld M, Chen YS (2011) Life-cycle analysis on biodiesel production from microalgae: water footprint and nutrients balance. Bioresour Technol 102:159–165. https://doi.org/10.1016/j.biortech.2010.07.017
Zhou Y, Schideman L, Yu G, Zhang Y (2013) A synergistic combination of algal wastewater treatment and hydrothermal biofuel production maximized by nutrient and carbon recycling. Energy Environ Sci 6:3765–3779. https://doi.org/10.1039/c3ee24241b
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The authors acknowledge the Department of Biotechnology and Indian Oil Corporation Ltd., Research & Development Centre, Faridabad, India for research support.
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PM: Conceptualization, Data collection-curation, Formal analysis, Methodology, Investigation, Writing—original draft, Writing—review and editing. RR: Formal analysis, Investigation, RG: Resources, review, statistical analysis, Funding acquisition. SKP and SSVR: Resources, review, Project administration, Final approval. Mathur: Investigation, Resources, interpretation of the data, review & editing.
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Mehta, P., Rani, R., Gupta, R. et al. Synergistic integration of wastewaters from second generation ethanol plant for algal biofuel production: an industrially relevant option. 3 Biotech 12, 34 (2022). https://doi.org/10.1007/s13205-021-03097-9
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DOI: https://doi.org/10.1007/s13205-021-03097-9