Abstract
Objective
Cholangiocarcinoma (CCA) is the second most common liver cancer, characterized by late diagnosis and fatal outcome. Although miR-192-5p has been shown to have a vital role in various cancers, its role in CCA is unknown. Here, we investigated the role of miR-192-5p in CCA cell proliferation and apoptosis, and elucidated its potential mechanism of action.
Methods
The miR-192-5p expression in CCA tissues and cell lines was detected by real-time quantitative reverse transcription-polymerase chain reaction. Cell proliferation was analyzed using the cell counting Kit-8 and 5-bromodeoxyuridine staining assays, while apoptosis was examined by flow cytometry and the terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling assay. Western blot analysis was used to measure the expression of cell proliferation and apoptosis-related proteins, as well as MEK/ERK signaling pathway-related proteins.
Results
MiR-192-5p was highly expressed in CCA tissues and cell lines. Overexpression of miR-192-5p significantly promoted CCA proliferation, and inhibited apoptosis. The MEK inhibitor, PD98059, reversed these miR-192-5p-induced effects on MEK/ERK signaling-associated protein expression, proliferation promotion, and apoptosis inhibition in TFK-1 cells.
Conclusion
MiR-192-5p promotes proliferation and suppressed apoptosis of CCA cells via the MEK/ERK pathway, which may be a potential therapeutic strategy for CCA treatment.
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Availability of data and materials
The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.
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CT and BC conceived and designed the project, CT, PY, JW and Yubo Zhang acquired the data, Xiaowei Chang, Dong Jin and Peng Lei analysed and interpreted the data, Zhenhui Lu and Bendong Chen wrote the paper.
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This study was approved by the hospital ethics committee. Informed consent was obtained from all participating subjects.
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Tang, C., Yuan, P., Wang, J. et al. MiR-192-5p regulates the proliferation and apoptosis of cholangiocarcinoma cells by activating MEK/ERK pathway. 3 Biotech 11, 99 (2021). https://doi.org/10.1007/s13205-021-02650-w
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DOI: https://doi.org/10.1007/s13205-021-02650-w