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Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate

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Abstract

An immobilization method for binding cross-linked enzyme aggregates of Lipozyme TL 100L on macroporous resin NKA (CLEA-TLL@NKA) was developed in this study. The esterification activity of CLEA-TLL@NKA reached 6.4 U/mg. The surface structure of immobilized lipase was characterized by scanning electron microscopy. Methyl esterification reaction of soybean oil deodorizer distillate (SODD) was catalyzed by CLEA-TLL@NKA, which the conversion rate reached 98% and its activity retained over 90% after 20 batches of reaction. Compared with the commercial enzyme Lipozyme TLIM, half-life (t1/2) of CLEA-TLL@NKA increased by 25 times and the catalytic activity increased by approximate 10 times. Thus, CLEA-TLL@NKA had high catalytic activity, good operational stability, and potential industrial application in the field of oil processing.

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Acknowledgements

This research was financially supported by National Natural Science Foundation of China (31600639, 31660247), key research and development program of Zhejiang Province (2019C01082) and the Education Department of Jiangxi Province (GJJ151211).

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Authors and Affiliations

  1. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, People’s Republic of China

    Jianyong Zheng, Wei Wei, Yinjun Zhang & Zhao Wang

  2. Zhejiang Medicine Co., Ltd Xinchang Pharmaceutical Factory, Xinchang, 312500, Zhejiang, People’s Republic of China

    Shengfan Wang

  3. School of Medicine and Life Sciences, Xinyu University, Xinyu, 338004, Jiangxi, People’s Republic of China

    Xiaojun Li

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  1. Jianyong Zheng
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  2. Wei Wei
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  3. Shengfan Wang
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  4. Xiaojun Li
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  5. Yinjun Zhang
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  6. Zhao Wang
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Contributions

Conceptualization, JZ and ZW; Data curation, JZ and ZW; Funding acquisition, JZ and XL; Investigation, WW, SW, XL and YZ; Supervision, ZW; Writing original draft, JZ and WW.

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Correspondence to Zhao Wang.

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Zheng, J., Wei, W., Wang, S. et al. Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate. 3 Biotech 10, 51 (2020). https://doi.org/10.1007/s13205-019-2028-6

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