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Effect of Glutaraldehyde Multipoint Covalent Treatments on Immobilized Lipase for Hydrolysis of Acidified Oil

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Abstract  

Immobilized lipase is a green and sustainable catalyst for hydrolysis of acidified oil. Glutaraldehyde is widely used for lipase immobilization while the appropriate strategy optimizes the catalytic performance of lipase. In this research, lipase from Candida rugosa (CRL) was immobilized on spherical silica (SiO2) by glutaraldehyde multipoint covalent treatments, including covalent binding method and adsorption-crosslinking method. The enzymatic stability properties and performance in hydrolysis of refined oil and acidified oil were studied. We confirmed that the residual activity decreased while the stability increased because of the influence on secondary structure of lipase after multipoint covalent treatments. In the comparison of different immobilization strategies in multipoint covalent treatment, SiO2-CRL (covalent binding method) showed lower loading capacity than SiO2-CRL (adsorption-crosslinking method), resulting in low activity. However, SiO2-CRL (covalent binding method) showed better reusability and stability. Immobilized lipase via covalent binding method was more potential in the application of catalytic hydrolysis of acidified oils.

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Funding

Financial support was provided by the Natural Science Foundation of China (NSFC) (No. 21978112) and MOE & SAFEA for the 111 Project (B13025).

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Authors and Affiliations

  1. The Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Xiulin Fan, Pingbo Zhang, Mingming Fan, Pingping Jiang & Yan Leng

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Contributions

All the authors contributed to the study conception and design. The main design of the article was performed by Pingbo Zhang. Material preparation, data collection, and analysis were performed by Mingming Fan and Pingping Jiang. The first draft of the manuscript was written by Xiulin Fan. All the authors commented on previous versions of the manuscript. All the authors read and approved the final manuscript.

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Correspondence to Pingbo Zhang.

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Fan, X., Zhang, P., Fan, M. et al. Effect of Glutaraldehyde Multipoint Covalent Treatments on Immobilized Lipase for Hydrolysis of Acidified Oil. Appl Biochem Biotechnol 195, 6942–6958 (2023). https://doi.org/10.1007/s12010-023-04477-y

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