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Antagonistic and plant-growth promoting novel Bacillus species from long-term organic farming soils from Sikkim, India

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Abstract

Three bacteria namely Bacillus luciferensis K2, Bacillus amyloliquefaciens K12 and Bacillus subtilis BioCWB possessing plant growth promotion and biocontrol potential against phytopathogens and rice leaf folder were identified from organic soils of Sikkim, India. The results revealed significant higher production of phytohormones IAA (97.1 μg mL−1) and GA3 (10.6 μg mL−1) was found in K2, whereas BioCWB had higher phosphate solubilization (570.0 μg mL−1) efficacy and also possessed nitrogen fixation ability (5.34 log copy number mL−1 culture). All these bacteria had higher antagonistic activities against phytopathogens viz. Rhizoctonia solani, Fusarium proliferatum, Athelia rolfsii and Colletotrichum gloeosporioides and also had higher larvicidal activity against rice leaf folder Cnaphalocrocis medinalis (Guenne) under in vitro conditions. Molecular insights into the antagonistic mechanisms of Bacillus strains deciphered the presence of several antimicrobial peptides (ericin, subtilin, surfactin, iturin, bacilysin, subtilosin, fengycin and bacillomycin), volatiles (dimethyl disulphide, methyl-Furan, acetic acid, Z-1,3-pentadiene and 3-hexyn-2-ol) and soluble metabolites (9-octadecenamide, E-15-heptadecenal, E-3-eicosene and 5-octadecene). Furthermore, liquid microbial inoculum prepared using the bacterial strains (K2, K12 and BioCWB) were evaluated under glass house (rice) and field condition (capsicum), which significantly enhanced plant growth in rice and yield in capsicum compared to control. The present study revealed the combination of Bacillus spp. (K2, K12 and BioCWB) can be used as bio-inoculants for improving agricultural production in Sikkim. Moreover, for the first time, we demonstrated plant growth promoting (PGP) traits, antifungal and insecticidal properties of B. luciferensis.

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Acknowledgements

The first author thanks Director, ICAR-National Rice Research Institute, Odisha, India for providing all the support for carrying out this research work and special thanks to DBT, India for financial support.

Funding

We are grateful to the Department of Biotechnology, Government of India for promotion of Biotechnology in the North Eastern Region of India through Biotech Consortium India Limited and giving grants (BT/PR16291/NER/95/185/2015) for this research work.

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Authors and Affiliations

  1. Crop Production Division, ICAR—National Rice Research Institute, Cuttack, 753006, Odisha, India

    Periyasamy Panneerselvam, Ansuman Senapati, Upendra Kumar, S. R. Prabhukarthikeyan, Afrin Jahan, Chidambaram Parameshwaran, Guru Prasana Pandi Govindharaj, Srikanta Lenka, Prafulla Kumar Nayak & Mahapatra Smruthi Sagarika

  2. Department of Horticulture, Sikkim University, Gangtok, 737102, Sikkim, India

    Laxuman Sharma, Pinky Lepcha & Debasis Mitra

  3. Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India

    Sugitha Thangappan & Utthandi Sivakumar

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  1. Periyasamy Panneerselvam
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Correspondence to Periyasamy Panneerselvam.

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Panneerselvam, P., Senapati, A., Kumar, U. et al. Antagonistic and plant-growth promoting novel Bacillus species from long-term organic farming soils from Sikkim, India. 3 Biotech 9, 416 (2019). https://doi.org/10.1007/s13205-019-1938-7

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