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Characterization of a 16SrII subgroup D phytoplasma strain associated with Calendula officinalis phyllody in Iran

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Abstract

Calendula officinalis plants with phyllody symptoms (CaoP) were observed in Yazd and Ashkezar (Yazd province, Iran) during 2013–2016. Twenty-one symptomatic and four asymptomatic plants were transferred individually to the greenhouse and potted for the biological and molecular characterization of associated phytoplasma. The dodder transmission from symptomatic potted marigold plants, induced virescence, phyllody and witches’ broom symptoms in periwinkle. Total DNAs extracted from symptomatic and symptomless plants and dodder-inoculated periwinkles were subjected to nested PCR assay using primer pairs amplifying phytoplasma ribosomal DNA. Expected PCR amplification was detected in all CaoP plant and dodder-inoculated periwinkle samples. RFLP analysis of the amplicons obtained in direct PCR with P1/P7 primers using RsaI, AluI, MseI, HinfI and HaeIII restriction enzymes showed profiles identical to each other and related to phytoplasmas in all the 21 positive samples. R16mF2/R16mR2 amplicons from six CaoP plant samples were sequenced where consensus sequences had 100% of identity among each other. R16F2n/R16R2-trimmed sequences (1248 bp) of representative samples from Yazd and Ashkezar were deposited in GenBank under accession numbers KU297202 and MH065715, respectively. BLAST search and phylogenetic analysis showed that the CaoP phytoplasma had 99% homology and clusters with phytoplasmas in group 16SrII. Computer-simulated analysis using iPhyClassifier suggests that the CaoP RFLP 16S rRNA gene pattern was identical to 16SrII-D phytoplasmas subgroup. Phytoplasma strains (16SrII-D) were reported as alfalfa witches’ broom disease agent previously in the same geographic areas, and it is possible that alfalfa plays a role in the epidemiology of CaoP disease or vice-versa.

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References

  • Asghari Tazehkand S, Hosseini Pour A, Heydarnejad J, Massumi H, Azadvar M (2010) Identification of phytoplasmas associated with cultivated and ornamental plants in Kerman province. Iran J Phytopathol 158(11–12):713–720

    Article  Google Scholar 

  • Babaie G, Khatabi B, Bayat H, Rastgou M, Hosseini A, Salekdeh GH (2007) Detection and characterization of phytoplasma infecting ornamental and weed plants in Iran. J Phytopathol 155(6):368–372

    Article  Google Scholar 

  • Bertaccini A, Duduk B (2009) Phytoplasma and phytoplasma diseases: a review of recent research. Phytopathol Mediterr 48:355–378

    CAS  Google Scholar 

  • Bertaccini A, Duduk B, Paltrinieri S, Contaldo N (2014) Phytoplasmas and phytoplasma diseases: a severe threat to agriculture. Am J Plant Sci 5:1763–1788

    Article  Google Scholar 

  • Chaturvedi Y, Rao G, Tiwari A, Duduk B, Bertaccini A (2010) Phytoplasma on ornamentals: detection, diversity and management. Acta Phytopathol Entomol 45(1):31–69

    Article  Google Scholar 

  • Deng S, Hiruki C (1991) Amplification of 16S rRNA genes from culturable and non-culturable mollicutes. J Microbiol Methods 14:53–61

    Article  CAS  Google Scholar 

  • Esmailzadeh Hosseini SA, Salehi M, Firooz R, Shamszadeh M (2008) Occurrence of marigold phyllody in Yazd province. In: 18th Iranian plant protection congress. Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran, p 418

  • Esmailzadeh Hosseini SA, Salehi M, Khanchezar A, Shamszadeh M (2011) The first report of a phytoplasma associated with pot marigold phyllody in Iran. Bull Insectol 64(Suppl):109–110

    Google Scholar 

  • Esmailzadeh Hosseini SA, Khodakaramian G, Salehi M, Fani SR, Bolok Yazdi HR, Raoufi D, Jadidi O, Bertaccini A (2015a) Status of alfalfa witches’ broom phytoplasma disease in Iran. Phytopathogenic Mollicutes 5(1-Suppl):65–66

    Article  Google Scholar 

  • Esmailzadeh Hosseini SA, Khodakaramian G, Salehi M, Fani SR, Mirchenari SM, Salehi E, Bertaccini A (2015b) Incidence, distribution and economic importance of alfalfa witches’ broom disease in Sistan–Baluchestan (Iran) and characterization of associated phytoplasma. Phytopathogenic Mollicutes 5(2):84–90

    Article  Google Scholar 

  • Esmailzadeh Hosseini SA, Salehi M, Khodakaramian G, Mirchenari SM, Bertaccini A (2015c) An up to date status of alfalfa witches’ broom disease in Iran. Phytopathogenic Mollicutes 5(1):9–18

    Article  Google Scholar 

  • Esmailzadeh Hosseini SA, Khodakaramian G, Salehi M, Bertaccini A (2016a) Characterization of 16SrII group phytoplasmas associated with alfalfa (Medicago sativa) witches’ broom disease in diverse areas of Iran. J Crop Prot 5(4):581–590

    Article  Google Scholar 

  • Esmailzadeh Hosseini SA, Khodakaramian G, Salehi M, Bertaccini A (2016b) First report of 16SrVI-A and 16SrXII-A phytoplasmas associated with alfalfa witches’ broom diseases in Iran. J Plant Pathol 98(2):369

    Google Scholar 

  • Esmailzadeh Hosseini SA, Khodakaramian G, Salehi M, Bertaccini A (2016c) Molecular identification and phylogenetic analysis of phytoplasmas associated with alfalfa witches’ broom diseases in the western areas of Iran. Phytopathogenic Mollicutes 6(1):16–22

    Article  Google Scholar 

  • Esmailzadeh Hosseini SA, Salehi M, Mirchenari SM, Bertaccini A (2016d) First report of a 16SrII-D phytoplasma associated with Calendula officinalis phyllody in Iran. New Dis Rep 34:22

    Article  Google Scholar 

  • Fattahi M, Salehi M, Sharzehi A, Esmailzadeh Hosseini SA (2016) Partial biological and molecular characteristics of a phytoplasma associated with Behshahr (Mazandaran) periwinkle phyllody. Iran J Plant Pathol 52(1):135–141

    Google Scholar 

  • Gera A, Maslenin L, Rosner A, Zeidan M, Weintraub PG (2006) Phytoplasma disease in ornamental crops in Israel. Acta Hortic 722:155–162

    Article  CAS  Google Scholar 

  • Gundersen DE, Lee I-M (1996) Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer sets. Phytopathol Mediterr 35:144–151

    CAS  Google Scholar 

  • Khan AJ, Botti S, Al-Subhi AM, Gundersen-Rindal DE, Bertaccini AF (2002) Molecular identification of a new phytoplasma associated with alfalfa witches’ broom in Oman. Phytopathology 92:1038–1047

    Article  CAS  PubMed  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874

    Article  CAS  PubMed  Google Scholar 

  • Lee I-M, Gundersen-Rindal DE, Davis RE, Bartoszyk IM (1998) Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int J Syst Evol Microbiol 48:1153–1169

    CAS  Google Scholar 

  • Lee I-M, Davis RE, Gundersen-Rindal DE (2000) Phytoplasma: phytopathogenic mollicutes. Ann Rev Microbiol 54:221–255

    Article  CAS  Google Scholar 

  • Marcone C, Ragozzino A, Seemüller E (1997) Detection and identification of phytoplasmas in yellows-diseased weeds in Italy. Plant Pathol 46:530–537

    Article  Google Scholar 

  • Mirzaie A, Esmailzadeh Hosseini SA, Jafari-Nodooshan A, Rahimian H (2007) Molecular characterization and potential insect vector of a phytoplasma associated with garden beet witches broom in Yazd, Iran. J Phytopathol 155(4):198–203

    Article  CAS  Google Scholar 

  • Omar AF, Alsohim AS (2016) Identification of new plant hosts of 16SrII group phytoplasmas in Saudi Arabia. Phytopathogenic Mollicutes 6(2):71–76

    Article  Google Scholar 

  • Rani A, Misra P, Singh J, Kumar P, Rani R, Shukla P (2014) PCR based detection of phytoplasma association in pot marigold (Calendula officinalis L.) and guldawari (Dendranthema grandiflora L.). Asian J Biol Sci 9(2):238–241

    Article  Google Scholar 

  • Saitou N, Nei M (1987) The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  • Salehi M, Izadpanah K, Siampour M, Esmailzadeh Hosseini SA (2011) Polyclonal antibodies for the detection and identification of Fars alfalfa witches’ broom phytoplasma. Bull Insectol 64(Suppl):59–60

    Google Scholar 

  • Salehi E, Salehi M, Taghavi SM, Izadpanah K (2014) 16SrII-D phytoplasma strain associated with tomato witches’ broom in Bushehr province. Iran J Crop Prot 3(3):377–388

    Google Scholar 

  • Salehi M, Esmailzadeh Hosseini SA, Salehi E (2015a) Characterisation of a phytoplasma associated with sunflower phyllody in Fars, Isfahan and Yazd provinces of Iran. New Dis Rep 3:6

    Article  Google Scholar 

  • Salehi M, Siampour M, Esmailzadeh Hosseini SA, Bertaccini A (2015b) Characterization and vector identification of phytoplasmas associated with cucumber and squash phyllody in Iran. Bull Insectol 68(2):311–319

    Google Scholar 

  • Salehi M, Esmailzadeh Hosseini SA, Rasoulpour R, Salehi E, Bertaccini A (2016a) Identification of a phytoplasma associated with pomegranate little leaf disease in Iran. Crop Prot 87:50–54

    Article  CAS  Google Scholar 

  • Salehi M, Esmailzadeh Hosseini SA, Salehi E, Bertaccini A (2016b) Molecular and biological characterization of a 16SrII phytoplasma associated with carrot witches’ broom in Iran. J Plant Pathol 98(1):83–90

    Google Scholar 

  • Salehi M, Esmailzadeh Hosseini SA, Salehi E, Bertaccini A (2016c) Genetic diversity and vector transmission of phytoplasmas associated with sesame phyllody in Iran. Folia Microbiol 62(2):99–109

    Article  CAS  Google Scholar 

  • Salehi M, Esmailzadeh Hosseini SA, Salehi E, Bertaccini A (2016d) Occurrence and characterization of a 16SrII-D subgroup phytoplasma associated with parsley witches’ broom disease in Iran. J Phytopathol 164(11–12):996–1002

    Article  CAS  Google Scholar 

  • Schneider B, Seemüller E, Smart CD, Kirkpatrick BC (1995) Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In: S Razin, Tully JG (eds) Molecular and diagnostic procedures in mycoplasmology. Academic Press, San Diego, pp 369–380

    Chapter  Google Scholar 

  • Wang K, Hiruki C (2001) Use of heteroduplex mobility assay for identification and differentiation of phytoplasmas in the aster yellows group and the clover proliferation group. Phytopathology 91:546–552

    Article  CAS  PubMed  Google Scholar 

  • Zhang YP, Uyemoto JK, Kirkpatrick BC (1998) A small-scale procedure for extracting nucleic acids from woody plants infected with various phytoplasmas for PCR assay. J Virol Methods 71:45–50

    Article  CAS  PubMed  Google Scholar 

  • Zhao Y, Wei W, Lee I-M, Shao J, Suo X, Davis RE (2009) Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). Int J Syst Evol Microbiol 59:2582–2593

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Center, AREEO, Yazd, Iran

    Seyyed Alireza Esmailzadeh Hosseini

  2. Plant Protection Research Department, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Zarghan, Iran

    Mohammad Salehi

  3. Plant Protection Research Department, Chaharmahal and Bakhtiari Agricultural and Natural Resources Research and Education Center, AREEO, Shahrekord, Iran

    Ghobad Babaie

  4. Department of Agricultural and Food Sciences (DISTAL), Alma Mater Studiorum, University of Bologna, Bologna, Italy

    Assunta Bertaccini

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  1. Seyyed Alireza Esmailzadeh Hosseini
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  2. Mohammad Salehi
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  3. Ghobad Babaie
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  4. Assunta Bertaccini
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Correspondence to Seyyed Alireza Esmailzadeh Hosseini.

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Esmailzadeh Hosseini, S.A., Salehi, M., Babaie, G. et al. Characterization of a 16SrII subgroup D phytoplasma strain associated with Calendula officinalis phyllody in Iran. 3 Biotech 8, 295 (2018). https://doi.org/10.1007/s13205-018-1320-1

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  • DOI: https://doi.org/10.1007/s13205-018-1320-1

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