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QTLs and analysis of the candidate gene for amylose, protein, and moisture content in rice (Oryza sativa L.)

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Abstract

In this study, we determined using NIRS the heritability percentage of amylose, protein, and moisture content in polished and unpolished rice in a CNDH population derived from a cross between Cheongcheong and Nagdong rice varieties. The results revealed a higher heritability percentage for the amylose content and compromised heritability for protein and moisture contents. We also conducted QTL analysis of rice for these major components and identified their chromosomal locations on a physical map. We found a total of four QTLs affecting the amylose, protein, and moisture contents of grain on chromosome 7. We constructed physical maps of seven DNA markers responsible for amylose content, six responsible for protein content, and three responsible for moisture content. Furthermore, we classified these genes according to their functions and found 17 genes (over 77%) to be involved in secondary metabolite synthesis, two genes (about 9%), each related to cell function and abiotic stress, and one gene (about 5%) involved in redox signaling.

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Acknowledgments

The authors are grateful for the financial assistance granted by the Next-Generation BioGreen21 Program (No. PJ011257012017), Rural Development Administration, Republic of Korea for this research. This research was supported by the Kyungpook National University Research Fund, 2016.

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Authors and Affiliations

  1. Department of Food Security and Agricultural Development, Kyungpook National University, Daegu, 41566, Korea

    Ester Bruno & Kyung–Min Kim

  2. Department of Pharmaceutical Science & Technology, Catholic University of Daegu, Gyeongsan-Si, Gyeongbuk, 38430, Korea

    Yun-Sik Choi

  3. Department of Biotechnology, Catholic University of Daegu, Gyeongsan-Si, Gyeongbuk, 38430, Korea

    Il Kyung Chung

  4. School of Applied Biosciences, College of Agriculture & Life Sciences, Kyungpook National University, Daegu, 41566, Korea

    Kyung–Min Kim

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  1. Ester Bruno
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  2. Yun-Sik Choi
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Correspondence to Kyung–Min Kim.

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Bruno, E., Choi, YS., Chung, I.K. et al. QTLs and analysis of the candidate gene for amylose, protein, and moisture content in rice (Oryza sativa L.). 3 Biotech 7, 40 (2017). https://doi.org/10.1007/s13205-017-0687-8

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