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Differential regulation of waxy gene expression in rice endosperm

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Summary

In order to examine the effects of different alleles on the gene expression at the waxy locus, the Wx gene product which controls the synthesis of amylose was isolated from endosperm starch of rice plants and analysed by electrophoretic techniques. The major protein bound to starch granules was absent in most of waxy strains and increased with the number of Wx alleles in triploid endosperms, suggesting that the major protein is the Wx gene product. In addition to wx alleles which result in the absence or drastic reduction of the Wx gene product and amylose, differentiation of Wx alleles seemed to have occurred among nonwaxy rice strains. At least two Wx alleles with different efficiencies in the production of the major protein as well as amylose were detected. These alleles are discussed in relation to regulation of the gene expression.

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References

  • Amano E (1980) Phenotypic expression of Wx gene in cereals. Annu Rep Natl Inst Genet, Misima 31:82–83

    Google Scholar 

  • Berg BM van den, Wrjsman HJW, Bianch F (1983) Genetics of the peroxidase isoenzyme in Petunia. 6. Differential temporal expression of prx B alleles. Theor Appl Genet 66: 173–178

    Google Scholar 

  • Bollich CN, Webb BD (1973) Inheritance of amylose of two hybrid populations of rice. Cereal Chem 50:631–636

    Google Scholar 

  • Echt CS, Schwartz D (1981) Evidence for the inclusion of controlling elements within the structural gene at the waxy locus in maize. Genetics 99:275–284

    Google Scholar 

  • Endo T (1981) Differential regulation of peroxidase isozymes coded by Px-1 locus in rice. Jpn J Genet 56:175–183

    Google Scholar 

  • Freeling M, Cheng DSK (1978) Radiation-induced alcohol dehydrogenase mutants in maize following allyl alcohol selection of pollen. Genet Res 31:107–129

    Google Scholar 

  • Ghosh AK, Govindaswamy S (1972) Inheritance of starch-iodine-blue value and alkali digestion value in rice and their genetic association. Il Riso 21:123–132

    Google Scholar 

  • Ikeno S (1914) Über die Bestäubung und die Bastardierung von Reis. Z Pflanzenzücht 2:495–503

    Google Scholar 

  • International Rice Research Institute (1974) Annu Rep 1973. Los Banos, Philippines, pp 3–9

  • International Rice Research Institute (1976) Annu Rep 1975. Los Banos, Philippines, pp 85–86

  • Iwata N, Omura T (1971) Linkage analysis by reciprocal translocation method in rice plants (Oryza sativa L.). 1. Linkage groups corresponding to the chromosome 1, 2, 3, and 4. Jpn J Breed 21:19–28

    Google Scholar 

  • Kudo M (1968) Genetical and breeding studies on physiological and ecological characters in hybrids between ecological groups of rice. Bull Natl Inst Agric Sci, Ser D 19:1–84

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685

    PubMed  Google Scholar 

  • Li HW, Wang S, Yeh PZ (1965) A preliminary note on the fine structure analysis of glutinous gene in rice. Bot Bull Acad Sin 6:101–105

    Google Scholar 

  • Li HW, Wu PH, Wu L, Chu MY (1968) Further studies of the interlocus recombination of the glutinous gene in rice. Bot Bull Acad Sin 9:22–26

    Google Scholar 

  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–274

    CAS  PubMed  Google Scholar 

  • McKenzie KS, Rutger JN (1983) Genetic analysis of amylose content, alkali spreading score, and grain dimensions in rice. Crop Sci 23:306–313

    Google Scholar 

  • Nagao S, Takahashi ME (1963) Trial construction of twelve linkage groups in Japanese rice (Genetical studies on rice plant XXVII). J Fac Agric, Hokkaido Univ 53:72–130

    Google Scholar 

  • Nelson OE, Chourey PS, Chang MT (1978) Nucleoside diphosphate sugar-starch glucosyl transferase activity of wx starch granules. Plant Physiol 62:383–386

    Google Scholar 

  • Nelson OE, Rines HW (1962) The enzymatic deficiency in the waxy mutant of maize. Biochem Biophys Res Commun 9:297–300

    Google Scholar 

  • O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

    Google Scholar 

  • Oka HI (1977) Genetic variations of Oryza glaberrima, their survey and evaluation. In: Meeting on African Rice Species, IRAT-ORSTOM, Paris, pp 77–82

    Google Scholar 

  • Okuno K (1978) Gene dosage effect of waxy alleles on amylose content in endosperm starch of rice. Jpn J Genet 53:219–222

    Google Scholar 

  • Okuno K, Fuwa H, Yano M (1983) A new mutant gene lowering amylose content in endosperm starch of rice. Jpn J Breed 33:387–394

    Google Scholar 

  • Sano Y (1978) Gene markers obtained in O. glaberrima. Annu Rep Natl Inst Genet, Misima, 29:97–98

    Google Scholar 

  • Satoh H, Omura T (1981) New endosperm mutations induced by chemical mutagens in rice, Oryza sativa L. Jpn J Breed 31:316–326

    Google Scholar 

  • Scandalios JG, Baum JA (1982) Regulatory gene variation in higher plants. Adv Genet 21:347–370

    Google Scholar 

  • Schwartz D (1966) The genetic control of alcohol dehydrogenase in maize: gene duplication and repression. Proc Natl Acad Sci USA 56:1431–1436

    Google Scholar 

  • Schwartz D, Endo T (1966) Alcohol dehydrogenase polymorphisms in maize-simple and compound loci. Genetics 53:709–715

    Google Scholar 

  • Schwartz D, Echt CS (1982) The effect of Ac dosage on the production of multiple forms of Wx protein by the wx m-9 controlling element mutation in maize. Mol Gen Genet 187:410–413

    Google Scholar 

  • Tsai CY (1974) The function of the waxy locus in starch synthesis in maize endosperm. Biochem Genet 11:83–96

    Google Scholar 

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Communicated by G. S. Khush

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Sano, Y. Differential regulation of waxy gene expression in rice endosperm. Theoret. Appl. Genetics 68, 467–473 (1984). https://doi.org/10.1007/BF00254822

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  • DOI: https://doi.org/10.1007/BF00254822

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