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Green synthesis of silver nanoparticles using Stevia leaves extracts

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Abstract

Three extracts of Stevia rebaudiana (Bertoni) were prepared using different types of raw materials: leaves of plants grown ex situ, leaves of plants grown in vitro, callus culture formed on damaged leaves. Composition of the extracts was studied by means of high-performance liquid chromatography and laser desorption/ionization mass spectrometry; total phenol content was estimated using Folin–Ciocalteau method. Flavonoids and hydroxycinnamic acids were found to be the main groups of phenol antioxidants available in the Stevia leaves, with the amount of these compounds in the extract being dependent on the type of raw material. The reducing properties of phenol compounds identified in the extracts were characterized using quantum chemical method; flavonoids and hydroxycinnamic acids were found to have similar redox parameters. Silver nanoparticles (AgNPs) colloids were synthesized using three Stevia extracts; AgNPs size distribution were characterized by means of scanning electron microscopy. All the extracts revealed significant activity in AgNPs synthesis; the nanoparticles of predominantly spherical shape with the average sizes of 16–25 nm were formed. The reducing properties of the extracts were found to correlate with total phenol content; the activity of extracts from the leaves of plants grown ex situ and from callus culture in Ag+ ions reduction was similar to each other and exceeded the activity of extract from the leaves of plants grown in vitro.

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Acknowledgements

Authors are grateful to Dr. V. Grakhov, DSc. J. Rakhmetov, Dr. O. Dzjuba, L. Shpak, the employees of M.M. Gryshko National Botanic Garden, National Academy of Sciences of Ukraine, for HPLC data and for providing Stevia extracts. Authors thank to Prof. V.M. Gun’ko, Department of Amorphous and Structurally Ordered Oxides, Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, for the use of the GAUSSIAN09 program suit.

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  1. Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, General Naumov Street, 17, Kiev, 03164, Ukraine

    Iryna Laguta, Oksana Stavinskaya, Olga Kazakova, Tetiana Fesenko & Sergey Brychka

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  1. Iryna Laguta
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  2. Oksana Stavinskaya
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Correspondence to Iryna Laguta.

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Laguta, I., Stavinskaya, O., Kazakova, O. et al. Green synthesis of silver nanoparticles using Stevia leaves extracts. Appl Nanosci 9, 755–765 (2019). https://doi.org/10.1007/s13204-018-0680-5

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