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Growth promoting effects of endophytic fungus Piriformospora indica in small cardamom (Elettaria cardamomum Maton)

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Abstract

Piriformospora indica, an endophytic fungus of Sebacinales, colonizes the roots of a wide range of host plants and provides various benefits to the plants. Cardamom (Elettaria cardamomum Maton) is an economically valuable spice crop of the tropics. In this work, we describe differentially expressed transcripts responding to P. indica root colonization in small cardamom for elucidation of molecular basis of growth and development. During the study, a wild genotype of cardamom was propagated under in vitro conditions using full strength Murashige and Skoog medium supplemented with 1 mg l−1 BAP (for shoot induction) and basal MS liquid medium (for root induction). Cardamom plantlets were co-cultivated with P. indica. Microscopic observation confirmed the presence of P. indica inside the roots of cardamom plantlets. Growth parameters of control and P. indica colonized plantlets were observed for three months at an interval of 15 days. P. indica colonization resulted in a significant increase in the morpho-physiological traits of the host plant. The growth enhancement was visible after 15 days of co-culture. There was a significant increase (p < 0.05) in the number and length of leaves, height of the plant and chlorophyll content in P. indica colonized plants compared to non-colonized control plant. In addition to this, the expression levels of auxin, nitrate reductase, vegetative storage protein and phosphate transporter genes were upregulated by 3.45, 3.26, 1.62 and 1.19 times respectively by the co-cultivation of P. indica in cardamom plantlets.

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Data Availability

Data availability The datasets generated during and analyzed during the current study are available from the corresponding author on reasonable request.

References

  • Abdelaziz ME, Atia MA, Abdelsattar M, Abdelaziz SM, Ibrahim TA, Abdeldaym EA (2021) Unravelling the role of Piriformospora indica in combating water deficiency by modulating physiological performance and chlorophyll metabolism-related genes in Cucumis sativus. Horticulturae 7(10):399

    Article  Google Scholar 

  • Achatz B, von Rüden S, Andrade D, Neumann E, Pons-Kühnemann J, Kogel KH, Franken P, Waller F (2010) Root colonization by Piriformospora indica enhances grain yield in barley under diverse nutrient regimes by accelerating early plant development. Plant Soil 333:59–70

    Article  CAS  Google Scholar 

  • Al-Zuhair H, El-Sayeh B, Ameen HA, Al-Shoora H (1996) Pharmacological studies of cardamom oil in animals. Pharmacol Res 34(1–2):79–82

    Article  CAS  PubMed  Google Scholar 

  • Alikulov B, Shurigin V, Davranov K, Ismailov Z (2022) Plant growth-promoting endophytic bacteria associated with Halocnemum strobilaceum (pall.) M.Bieb and their plant beneficial traits. Plant Sci Today 8(sp1):44–50. https://doi.org/10.14719/pst.1605

    Article  Google Scholar 

  • Anith KN, Aswini S, Varkey S, Radhakrishnan NV, Nair DS (2018) Root colonization by the endophytic fungus Piriformospora indica improves growth, yield and piperine content in black peer (Piper Nigurm L). Biocatal Agric Biotechnol 14:215–220

    Article  Google Scholar 

  • Anith KN, Faseela KM, Archana PA, Prathapan KD (2011) Compatibility of Piriformospora indica and Trichoderma Harzianum as dual inoculants in black pepper (Piper nigrum L). Symbiosis 55:11–17

    Article  Google Scholar 

  • Anith KN, Sreekumar A, Sreekumar J (2015) The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus. Symbiosis 65(1):9–16

    Article  CAS  Google Scholar 

  • Arnon D (1949) Copper enzyme in isolated chloroplast and chlorophyll expressed in terms of mg per gram. Plant Physiol 24:15–23

    Google Scholar 

  • Ashokkumar K, Murugan M, Dhanya MK, Warkentin TD (2020) Botany, traditional uses, phytochemistry and biological activities of cardamom [Elettaria cardamomum (L.) Maton]-A critical review. J Ethnopharmacol 246:112244

    Article  CAS  PubMed  Google Scholar 

  • Athira S, Anith KN (2020) Plant growth promotion and suppression of bacterial wilt incidence in tomato by rhizobacteria, bacterial endophytes and the root endophytic fungus Piriformospora indica. Indian Phytopathol 73:629–642

    Article  Google Scholar 

  • Babu M, Radhika M, Sehgal PK (1999) Cellular proliferation on desamidated collagen matrices. Comp Biochem Physiol C Toxicol Pharmacol 124(2):131–139

    Google Scholar 

  • Bakshi M, Sherameti I, Meichsner D, Thürich J, Varma A, Johri AK, Yeh KW, Oelmüller R (2017) Piriformospora indica reprograms gene expression in Arabidopsis phosphate metabolism mutants but does not compensate for phosphate limitation. Front Microbiol 8:1262

    Article  PubMed  PubMed Central  Google Scholar 

  • Bandyopadhyay P, Yadav BG, Kumar SG, Kumar R, Kogel KH, Kumar S (2022) Piriformospora indica and Azotobacter chroococcum Consortium facilitates higher Acquisition of N, P with Improved Carbon Allocation and enhanced plant growth in Oryza sativa. J Fungi 8:453

    Article  CAS  Google Scholar 

  • Barazani O, Benderoth M, Groten K, Kuhlemeier N, Baldwin IT (2005) Piriformospora indica and Sebacina Vermifera increase growth performance at the expense of herbivore resistance in Nicotiana attenuate. Oecologia 146:234–243

    Article  PubMed  Google Scholar 

  • Chandrappa HM, Shanthaveerabhadraiah SM, Jagannath B (1997) Response of cardamom (Elettaria cardamomum Maton) to NPK under uniform shade. J Spices Aromat Crops 6(2):115–118

    Google Scholar 

  • Choudhury D, Tarafdar S, Parvin N, Rit R, Roy S, Sadhu S, Dutta S (2023) Endophytic microbes and their diverse beneficial aspects in various sectors: a critical insight. Plant Sci Today 10(1):96–107. https://doi.org/10.14719/pst.1877

    Article  CAS  Google Scholar 

  • Conchillo LB, Haro R, Benito B (2021) K+ nutrition exchange in the serendipita-Arabidopsis symbiosis: study of the fungal K+ transporters involved front. Ecol Evol 9:876

    Google Scholar 

  • Contreras-Cornejo HA, Macias-Rodriguez L, Corte-Penagos C, Lopez-Bucio J (2009) Trichoderma virens, a plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in Arabidopsis. Plant Physiol 149:1579–1592

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Croft H, Chen JM, Luo X, Bartlett P, Chen B, Staebler RM (2017) Leaf chlorophyll content as a proxy for leaf photosynthetic capacity. Glob Chang Biol 23:3513–3524

    Article  PubMed  Google Scholar 

  • Cruz C, Fegghi Z, Martins-Loução MA, Varma A (2013) Plant nitrogen use efficiency may be improved through symbiosis with Piriformospora indica. In: Varma A, Kost G, Oelmüller R (eds) Piriformospora indica: sebacinales and their biotechnological applications; Soil Biology, vol 33. Springer, Berlin (Heidelberg), pp 285–293

  • Dai Y, Shen Z, Liu Y, Wang L, Hannaway D, Lu H (2009) Effects of shade treatments on the photosynthetic capacity, chlorophyll fluorescence, and chlorophyll content of Tetrastigma Hemsleyanum Diels et Gilg. Environ Exp Bot 65(2–3):177–182

    Article  CAS  Google Scholar 

  • Daneshkhah R, Cabello S, Rozanska E, Sobczak M, Grundler FMW, Wieczorek K, Hofmann J (2013) Piriformospora indica antagonizes cyst nematode Infection and development in Arabidopsis roots. J Exp Bot 64(12):3763–3774

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Das A, Kamal S, Shakil NA, Sherameti I, Oelmüller R, Dua M, Tuteja N, Johri AK, Varma A (2012) The root endophyte fungus Piriformospora indica leads to early flowering, higher biomass and altered secondary metabolites of the medicinal plant, Coleus Forskohlii. Plant Signal Behav 7(1):103–112

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Deshmukh SD, Kogel KH (2007) Piriformospora indica protects barley from root rot caused by Fusarium Graminearum. J Plant Dis Prot 114(6):263–268

    Article  Google Scholar 

  • Fujiwara T, Nambara E, Yamagishi K, Goto DB, Naito S (2002) Storage proteins. The Arabidopsis Book/American Society of Plant Biologists 1

  • Hemavathy AT, Balaji K (2010) In vitro propagation studies in cardamom. Crop Res (Hisar) 40(1/3):217–220

    Google Scholar 

  • Hussain A, Qarshi IA, Nazir H, Ullah I (2012) Plant tissue culture: current status and opportunities. Recent Adv Plant vitro Cult 6(10):1–28

    Google Scholar 

  • Idowu PE, Ibitoye DO, Ademoyegun OT (2009) Tissue culture as a plant production technique for horticultural crops. Afr J Biotechnol 8(16):3782–3788

    Google Scholar 

  • Jisha S, Sabu KK, Manjula S (2019) Multifunctional aspects of Piriformospora indica in plant endosymbiosis. Mycol 10(3):182

    Article  CAS  Google Scholar 

  • Jogawat A, Meena MK, Kundu A, Varma M, Vadassery J (2020) Calcium channel CNGC19 mediates basal defense signaling to regulate colonization by Piriformospora indica in Arabidopsis roots. J Exp Bot 71:2752–2768

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Khan AU, Khan QJ, Gilani AH (2011) Pharmacological basis for the medicinal use of cardamom in Asthma. Bangladesh J Pharmacol 6(1):34–37

    Article  Google Scholar 

  • Kilam D, Saifi M, Abdin MZ, Agnihotri A, Varma A (2015) Combined effects of Piriformospora indica and Azotobacter chroococcum enhance plant growth, antioxidant potential and steviol glycoside content in Stevia rebaudiana. Symbiosis 66:149–156

    Article  CAS  Google Scholar 

  • Kim D, Abdelaziz ME, Ntui VO, Guo X, Al-Babili S (2017) Colonization by the endophyte Piriformospora indica leads to early flowering in Arabidopsis thaliana likely by triggering gibberellin biosynthesis. Biochem Biophys Res Commun 490:1162–1167

    Article  CAS  PubMed  Google Scholar 

  • Kumar M, Yadav V, Kumar H, Sharma R, Singh A, Tuteja N, Johri AK (2011) Piriformospora indica enhances plant growth by transferring phosphate. Plant Signal Behav 6:723–725

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kundu A, Mishra S, Kundu P, Jogawat A, Vadassery J (2022) Piriformospora indica recruits host-derived putrescine for growth promotion in plants. Plant Physiol 188(4):2289–2307

    Article  CAS  PubMed  Google Scholar 

  • Kushwaha A, Goswami L, Lee J, Sonne C, Brown RJ, Kim KH (2022) Selenium in soil-microbe-plant systems: Sources, distribution, toxicity, tolerance, and detoxification. Crit Rev Environ Sci Technol 52(13):2383–2420

    Article  CAS  Google Scholar 

  • Lakshmipriya P, Nath V, Veena SS, Anith KN, Sreekumar J, Jeeva ML (2016) Piriformospora indica, a Cultivable Endophyte for Growth Promotion and Disease Management in Taro (Colocasia esculenta L). J Root Crops 42(2):107–114

  • Lekshmi RS, Sora S, Anith KN, Soniya EV (2022) Root colonization by the endophytic fungus Piriformospora indica shortens the juvenile phase of Piper nigrum L. by fine tuning the floral promotion pathways. Front Plant Sci 13:954693

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Leyser O (2006) Dynamic integration of auxin transport and signaling. Curr Biol 16:424–433

    Article  Google Scholar 

  • Li Y, He N, HouJ, Xu L, Liu C, Zhang J, Wang Q, Zhang X, Wu X (2018) Factors influencing leaf chlorophyll content in natural forests at the biome scale. Front Ecol Evol 6:64

    Article  CAS  Google Scholar 

  • Li D, Mensah RA, Liu F, Tian N, Qi Q, Yeh K, Xuhan X, Cheng C, Lai Z (2019) Effects of Piriformospora indica on rooting and growth of tissue-cultured banana (Musa acuminata Cv. Tianbaojiao) seedlings. Scientia Hort 257:108649

    Article  Google Scholar 

  • Li X, Wei Y, Acharya A, Jiang Q, Kang J, Brummer EC (2017) A saturated genetic linkage map of autotetraploid alfalfa (Medicago sativa L.) developed using genotyping-by-sequencing is highly syntenous with the Medicago truncatula genome. Genes Genom Genet G3(10):1971–1979

    Google Scholar 

  • Lin HF, Xiong J, Zhou HM, Chen CM, Lin FZ, Xu XM, Oelmüller R, Xu WF, Yeh KW (2019) Growth promotion and Disease resistance induced in Anthurium colonized by the beneficial root endophyte Piriformospora indica. BMC Plant Biol 19:40

    Article  PubMed  PubMed Central  Google Scholar 

  • Liu F, Chang X, Ye Y, Xie W, Wu P, Lian X (2011) Comprehensive sequence and whole-life-cycle expression profile analysis of the phosphate transporter gene family in rice. Mol Plant 4:1105–1122

    Article  CAS  PubMed  Google Scholar 

  • Madhusoodanan KJ, Kumar KP, Ravindran PN (2002) Botany, crop improvement and biotechnology of cardamom. In: Taylor and Francis (eds.), Cardamom-The genus Elettaria. Taylor & Francis Inc, London, pp 11–68

  • Malhotra EV, Kamalapriya M, Bansal S, Meena DPS, Agrawal A (2021) Improved protocol for micropropagation of genetically uniform plants of commercially important cardamom (Elettaria cardamomum Maton). In Vitro Cell Dev Biol P 57(3):409–417

    Article  CAS  Google Scholar 

  • Meents AK, Furch AC, Almeida-Trapp M, Özyürek S, Scholz SS, Kirbis A, Lenser T, Theißen G, Grabe V, Hansson B, Mithöfer A (2019) Beneficial and pathogenic Arabidopsis root-interacting fungi differently affect auxin levels and responsive genes during early Infection. Front Microbiol 10:380

    Article  PubMed  PubMed Central  Google Scholar 

  • Mishra AK, Dwivedi SK, Kishore N, Dubey NK (1991) Fungistatic properties of essential oils of cardamom. Int J Pharmacogn 29:259–262

    Article  CAS  Google Scholar 

  • Muchhal US, Pardo JM, Raghothama KG (1996) Phosphate transporters from the higher plant Arabidopsis thaliana. Proc Natl Acad Sci 93(19):10519–10523

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with Tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nadiya F, Anjali N, Gangaprasad A, Sabu KK (2015) High-quality RNA extraction from small cardamom tissues rich in polysaccharides and polyphenols. Anal Biochem 485:25–27

    Article  CAS  PubMed  Google Scholar 

  • Nandana MS, Anith KN (2020) Growth promotion in Chilli (Capsicum annuum L.) on inoculation with co-cultured Piriformospora indica and Pseudomonas fluorescens. Int J Curr Microbiol Appl Sci 9:2015–2027

    Article  CAS  Google Scholar 

  • Narayan OP, Verma N, Jogawat A, Dua M, Johri AK (2021) Sulfur transfer from the endophytic fungus Serendipita indica improves maize growth and requires the sulfate transporter SiSulT. Plant Cell 33:1268–1285

    Article  PubMed  Google Scholar 

  • Olivé I, Brun FG, Vergara JJ, Pérez-Lloréns JL (2007) Effects of light and biomass partitioning on growth, photosynthesis and carbohydrate content of the seagrass Zosteara Noltii Hornem. J Exp Mar Biol Ecol 345(2):90–100

    Article  Google Scholar 

  • Pan R, Xu L, Wei Q, Wu C, Tang W, Oelmüller R, Zhang W (2017) Piriformospora indica promotes early flowering in Arabidopsis through regulation of the photoperiod and gibberellin pathways. PLoS ONE 12:e0189791. https://doi.org/10.1371/journal.pone.0189791

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Paul T, Nysanth NS, Yashaswini MS, Anith KN (2021) Inoculation with bacterial endophytes and the fungal root endophyte, Piriformospora indica improves plant growth and reduces foliar Infection by Phytophthora capsici in black pepper. J Trop Agric 59:224–235

    CAS  Google Scholar 

  • Pedersen BP, Kumar H, Waight AB, Risenmay AJ, Roe-Zurz Z, Chau BH, Schlessinger A, Bonomi M, Harries W, Sali A, Johri AK (2013) Crystal structure of a eukaryotic phosphate transporter. Nature 496(7446):533–536

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Prasad R, Kamal S, Sharma PK, Oelmueller R, Varma A (2013) Root endophyte Piriformospora indica DSM 11827 alters plants morphology, enhances biomass and antioxidant activity of medicinal plant Bacopa monniera. J Basic Microbiol 53:1016–1024

    Article  CAS  PubMed  Google Scholar 

  • Rai M, Acharya D, Singh A, Varma A (2001) Positive growth responses of the medicinal plants Spilanthes calva and Withania somnifera to inoculation by Piriformospora indica in a field trial. Mycorr 11:123–128

    Article  Google Scholar 

  • Rao NKS, Narayanaswamy S, Chacko EK, Swamy RD (1982) Regeneration of plantlets from callus of Elettaria cardamomum Maton. Proc Indian Acad Sci (Plant Sci) 91:37–41

    Article  Google Scholar 

  • Reghunath BR, Gopalakrishnan PK (1991) Successful exploitation of in vitro culture techniques for rapid clonal multiplication and crop improvement in cardamom. Proc. of 3rd Kerala Sci. Cong., held at Kozhikode, Kerala: 70–71

  • Sakuraba Y, Kim D, Paek NC (2018) Salt treatments and induction of Senescence. In: Guo Y (ed) Plant Senescence; methods in Molecular, Biology, vol 1744. Humana Press, New York, NY, USA, pp 141–149

    Google Scholar 

  • Schachtman DP, Reid RJ, Ayling SM (1998) Phosphorus uptake by plants: from soil to cell. Plant Physiol 116(2):447–453

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shahollari B, Varma A, Oelmuller R (2005) Expression of a receptor kinase in Arabidopsis roots is stimulated by the basidiomycete Piriformospora indica and the protein accumulates in Triton X- 100 insoluble plasma membrane microdomains. J Plant Physiol 162:945–958

    Article  CAS  PubMed  Google Scholar 

  • Sherameti I, Shahollari B, Venus Y, Altschmied L, Varma A, Oelmüller R (2005) The endophytic fungus Piriformospoa indica stimulates the expression of nitrate reductase and the starch degrading enzyme glucan-water dikinase in Tobacco and Arabidopsis roots through a homodomain transcription factor that binds to conserved motif in the promoters. J Biol Chem 280:26241–26247

    Article  CAS  PubMed  Google Scholar 

  • Sherameti I, Tripathi S, Varma A, Oelmuller R (2008a) The root colonizing endophyte Pirifomospora indica confers drought tolerance in Arabidopsis by stimulating the expression of drought stress related genes in leaves. Mol Plant Microbe Interact 21:799–807

    Article  CAS  PubMed  Google Scholar 

  • Sherameti I, Venus Y, Drzewiecki C, Nitz I (2008b) PYK10, a β -glucosidase located in the endoplasmatic reticulum, is crucial for the beneficial interaction between Arabidopsis thaliana and the endophytic fungus Piriformospora indica. Plant J 54:428–439

    Article  CAS  PubMed  Google Scholar 

  • Shukla J, Mohd S, Kushwaha AS, Narayan S, Saxena PN, Bahadur L, Mishra A, Shirke PA, Kumar M (2022) Endophytic fungus serendipita indica reduces arsenic mobilization from root to fruit in colonized tomato plant. Environ Poll 298:118830

    Article  CAS  Google Scholar 

  • Sirrenberg A, Goebel C, Grond S, Pawlowski K (2007) Piriformospora indica affects plant growth by auxin production. Plant Physiol 131:581–589

    Article  CAS  Google Scholar 

  • Tangjang A, Sharma A (2018) Marketing pattern of large cardamom (Amomum Sabulatum) in Tirap District of Arunachal Pradesh, India. Int J Curr Microbiol App Sci 7(05). http://www.ijcmas.com 

  • Urumbil SK, Anilkumar M (2021) Metagenomic insights into plant growth promoting genes inherent in bacterial endophytes of Emilia sonchifolia (Linn.) DC. Plant Sci Today 8(sp1):6–16

    Article  CAS  Google Scholar 

  • Varkey S, Anith KN, Narayana R, Aswini S (2018) A consortium of rhizobacteria and fungal endophyte suppress the root-knot nematode parasite in tomato. Rhizosphere 5:38–42

    Article  Google Scholar 

  • Varma A, Sree KS, Arora M, Bajaj R, Prasad R, Kharkwal AC (2012) Functions of novel symbiotic fungus - piriformospora indica. Proc Indian Natl Sci Acad 80:429–441

    Article  Google Scholar 

  • Varma A, Sree KS, Arora M, Bajaj R, Prasad R, Kharkwal AC (2014) Functions of novel symbiotic fungus - piriformospora indica. Proc Indian Natl Sci Acad 80:429–441

    Article  Google Scholar 

  • Verma K, Jain V, Katewa SS (2009) Blood pressure lowering, fibrinolysis enhancing and antioxidant activities of cardamom (Elettaria cardamomum). Indian J Biochem Biophys 46:503–506

    CAS  PubMed  Google Scholar 

  • Weiss M, Selosse MA, Rexer KH, Urban A, Oberwinkler F (2004) Sebacinales: a hitherto overlooked cosm of heterobasidiomycetes with a broad mycorrhyzal potential. Mycol Res 108:1003–1010

    Article  PubMed  Google Scholar 

  • Winarsi H, Sasongko ND, Purwanto A, Nuraeni I (2014) Effect of cardamom leaves extract as antidiabetic, weight lost and hypocholesterolemic to alloxan induced Sprague Dawley diabetic rats. Int Food Res J 21(6):2253

    Google Scholar 

  • Xu L, Aiai W, Jun W, Qiao W, Wenying Z (2017) Piriformospora indica confers drought tolerance on Zea mays L. through enhancement of antioxidant activity and expression of drought-related genes. Crop J 3:251–258

    Article  Google Scholar 

  • Yadav V, Kumar M, Deep DK, Kumar H, Sharma R, Tripathi T, Tuteja N, Saxena AK, Johri AK (2010) A phosphate transporter from the root endophytic fungus Piriformospora indica plays a role in phosphate transport to the host plant. J Biol Chem 285:26532–22654

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

Authors acknowledge A. S. Hemanthakumar, Technical Officer, JNTBGRI for developing initial in vitro cultures of cardamom.

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  1. H. N. Anjana

    Present address: Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Palode, 695562, Thiruvananthapuram, India

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  1. Department of Agricultural Microbiology, College of Agriculture, Kerala Agricultural University, Vellayani, 695522, Thiruvananthapuram, India

    H. N. Anjana & K. N. Anith

  2. Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Palode, 695562, Thiruvananthapuram, India

    K. K. Sabu

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Anjana, H.N., Anith, K.N. & Sabu, K.K. Growth promoting effects of endophytic fungus Piriformospora indica in small cardamom (Elettaria cardamomum Maton). Symbiosis 91, 79–89 (2023). https://doi.org/10.1007/s13199-023-00949-1

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