Abstract
Tea is a natural dietary supplement rich in polyphenols and based on the manufacturing process, their polyphenol content also varies. In the present study, we have compared the in vitro antioxidant, anticancer and anti-inflammatory activities of green tea (GT), orthodox black tea (oBT) and CTC black tea (cBT). The analysis was carried out in 50:50 ethanol:water extracts. The total antioxidant capacity, total polyphenol content and free radical scavenging activity were found to be high in GT samples. HPLC profiling indicated a higher percentage of polyphenols like catechin, epicatechin, epigallocatechin and epigallocatechin-gallate in GT when compared to other samples. The comparison of the anticancer potential was done in breast cancer MDA MB-231 cells and it was found that GT has a higher percentage of cell growth inhibition than oBT and cBT. Anti-inflammatory effects were done in LPS stimulated RAW264.7 macrophage cells and here also GT showed maximum effects. This was confirmed by the lower production of iNOS, reduced level of ROS generation and proinflammatory cytokines such as MCP-1, IL-1ɑ, and IL-6 by GT. To conclude, the order for the biological effectiveness of different teas tested is in the order GT > oBT > cBT.
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Authors thank Council of Scientific and Industrial Research (CSIR), India for the financial support for the project (Immunity mission project HCP-35).
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Arpan Singha Deo and Asheela Devi PJ did the extraction, antioxidant and anti-inflammatory studies and made the first draft of the paper. Anusha R did the anticancer studies. Sijisha KS and Tripti Mishra were involved in the HPLC analysis of the extracts. Siby Mathew, K Mathew Abraham and Jagadish R were involved in the sample collection from the industry, necessary documentation and correction of the manuscript. Priya S took the overall responsibility of conceptualization of idea, experimental planning and monitoring, fund generation, final correction and editing of the paper. All the authors have read and approved the final manuscript.
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Deo, A.S., Devi, P.J.A., Sijisha, K.S. et al. Comparative studies on the antioxidant, anticancer and anti-inflammatory activities of green tea, orthodox black tea and CTC black tea. J Food Sci Technol 61, 1315–1325 (2024). https://doi.org/10.1007/s13197-023-05900-2
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DOI: https://doi.org/10.1007/s13197-023-05900-2